Experiment: Protocatechuic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05817 and H281DRAFT_05818 are separated by 32 nucleotides H281DRAFT_05818 and H281DRAFT_05819 overlap by 4 nucleotides H281DRAFT_05819 and H281DRAFT_05820 are separated by 83 nucleotides H281DRAFT_05820 and H281DRAFT_05821 are separated by 16 nucleotides
H281DRAFT_05817: H281DRAFT_05817 - NADH dehydrogenase, at 94,285 to 95,238
_05817
H281DRAFT_05818: H281DRAFT_05818 - glutathione S-transferase, at 95,271 to 95,924
_05818
H281DRAFT_05819: H281DRAFT_05819 - tRNA nucleotidyltransferase (CCA-adding enzyme), at 95,921 to 97,153
_05819
H281DRAFT_05820: H281DRAFT_05820 - Protein of unknown function (DUF2905), at 97,237 to 97,431
_05820
H281DRAFT_05821: H281DRAFT_05821 - SAM-dependent methyltransferase, MidA family, at 97,448 to 98,638
_05821
Position (kb) 95
96
97
98 Strain fitness (log2 ratio) -1
0
1 at 94.940 kb on - strand, within H281DRAFT_05817 at 94.964 kb on - strand, within H281DRAFT_05817 at 95.260 kb on + strand at 95.260 kb on + strand at 95.260 kb on + strand at 95.261 kb on - strand at 95.261 kb on - strand at 95.282 kb on + strand at 95.282 kb on + strand at 95.282 kb on + strand at 95.282 kb on + strand at 95.282 kb on + strand at 95.298 kb on + strand at 95.609 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.715 kb on + strand, within H281DRAFT_05818 at 95.716 kb on - strand, within H281DRAFT_05818 at 95.716 kb on - strand, within H281DRAFT_05818 at 95.763 kb on + strand, within H281DRAFT_05818 at 95.763 kb on + strand, within H281DRAFT_05818 at 95.764 kb on - strand, within H281DRAFT_05818 at 95.764 kb on - strand, within H281DRAFT_05818 at 95.826 kb on + strand, within H281DRAFT_05818 at 95.827 kb on - strand, within H281DRAFT_05818 at 95.828 kb on + strand, within H281DRAFT_05818 at 95.828 kb on + strand, within H281DRAFT_05818 at 95.904 kb on + strand at 95.905 kb on - strand at 95.908 kb on + strand at 95.908 kb on + strand at 95.926 kb on + strand at 95.926 kb on + strand at 95.930 kb on + strand at 95.930 kb on + strand at 96.727 kb on + strand, within H281DRAFT_05819 at 96.742 kb on + strand, within H281DRAFT_05819 at 97.214 kb on + strand at 97.214 kb on + strand at 97.215 kb on - strand at 97.215 kb on - strand at 97.239 kb on - strand at 97.290 kb on + strand, within H281DRAFT_05820 at 97.290 kb on + strand, within H281DRAFT_05820 at 97.304 kb on + strand, within H281DRAFT_05820 at 97.304 kb on + strand, within H281DRAFT_05820 at 97.449 kb on + strand at 97.450 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Protocatechuic Acid (C) remove 94,940 - H281DRAFT_05817 0.69 +0.2 94,964 - H281DRAFT_05817 0.71 +0.1 95,260 + +1.4 95,260 + -0.4 95,260 + +0.3 95,261 - +0.2 95,261 - -0.8 95,282 + +0.0 95,282 + -0.4 95,282 + -0.3 95,282 + -0.2 95,282 + +0.5 95,298 + -0.2 95,609 + H281DRAFT_05818 0.52 -0.1 95,715 + H281DRAFT_05818 0.68 +0.6 95,715 + H281DRAFT_05818 0.68 -0.0 95,715 + H281DRAFT_05818 0.68 +0.4 95,715 + H281DRAFT_05818 0.68 +0.6 95,715 + H281DRAFT_05818 0.68 -0.1 95,715 + H281DRAFT_05818 0.68 -0.1 95,716 - H281DRAFT_05818 0.68 +0.2 95,716 - H281DRAFT_05818 0.68 -1.2 95,763 + H281DRAFT_05818 0.75 -0.2 95,763 + H281DRAFT_05818 0.75 -0.5 95,764 - H281DRAFT_05818 0.75 -1.2 95,764 - H281DRAFT_05818 0.75 +0.4 95,826 + H281DRAFT_05818 0.85 +0.4 95,827 - H281DRAFT_05818 0.85 +0.4 95,828 + H281DRAFT_05818 0.85 -0.3 95,828 + H281DRAFT_05818 0.85 -0.1 95,904 + +0.0 95,905 - -0.3 95,908 + -0.1 95,908 + -0.8 95,926 + -0.4 95,926 + -1.2 95,930 + +0.1 95,930 + +0.1 96,727 + H281DRAFT_05819 0.65 +0.4 96,742 + H281DRAFT_05819 0.67 +0.4 97,214 + +0.3 97,214 + +1.1 97,215 - +0.4 97,215 - -0.5 97,239 - -0.7 97,290 + H281DRAFT_05820 0.27 +0.0 97,290 + H281DRAFT_05820 0.27 +0.1 97,304 + H281DRAFT_05820 0.34 +0.4 97,304 + H281DRAFT_05820 0.34 +0.5 97,449 + +0.2 97,450 - +0.1
Or see this region's nucleotide sequence