Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3516 and Dsui_3517 overlap by 4 nucleotides Dsui_3517 and Dsui_3518 are separated by 84 nucleotides Dsui_3518 and Dsui_3519 are separated by 72 nucleotides
Dsui_3516: Dsui_3516 - hypothetical protein, at 3,779,723 to 3,781,249
_3516
Dsui_3517: Dsui_3517 - putative membrane protein, at 3,781,246 to 3,782,076
_3517
Dsui_3518: Dsui_3518 - putative membrane protein, at 3,782,161 to 3,782,523
_3518
Dsui_3519: Dsui_3519 - 2-isopropylmalate synthase, bacterial type, at 3,782,596 to 3,784,137
_3519
Position (kb)
3782
3783 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3781.179 kb on + strand at 3781.187 kb on - strand at 3781.207 kb on + strand at 3781.221 kb on - strand at 3781.315 kb on + strand at 3781.327 kb on - strand at 3781.371 kb on + strand, within Dsui_3517 at 3781.379 kb on - strand, within Dsui_3517 at 3781.386 kb on - strand, within Dsui_3517 at 3781.575 kb on + strand, within Dsui_3517 at 3781.605 kb on + strand, within Dsui_3517 at 3781.605 kb on + strand, within Dsui_3517 at 3781.643 kb on + strand, within Dsui_3517 at 3781.728 kb on - strand, within Dsui_3517 at 3781.750 kb on + strand, within Dsui_3517 at 3781.773 kb on + strand, within Dsui_3517 at 3781.773 kb on + strand, within Dsui_3517 at 3781.820 kb on + strand, within Dsui_3517 at 3781.823 kb on - strand, within Dsui_3517 at 3781.829 kb on - strand, within Dsui_3517 at 3781.879 kb on + strand at 3781.880 kb on + strand at 3781.880 kb on + strand, within Dsui_3517 at 3781.880 kb on + strand at 3781.880 kb on + strand, within Dsui_3517 at 3781.888 kb on - strand, within Dsui_3517 at 3781.888 kb on - strand, within Dsui_3517 at 3781.888 kb on - strand, within Dsui_3517 at 3781.888 kb on - strand, within Dsui_3517 at 3781.965 kb on + strand, within Dsui_3517 at 3781.973 kb on + strand, within Dsui_3517 at 3781.973 kb on + strand, within Dsui_3517 at 3781.981 kb on - strand, within Dsui_3517 at 3781.981 kb on - strand, within Dsui_3517 at 3782.015 kb on - strand at 3782.065 kb on - strand at 3782.065 kb on - strand at 3782.065 kb on - strand at 3782.109 kb on + strand at 3782.109 kb on + strand at 3782.122 kb on - strand at 3782.171 kb on + strand at 3782.232 kb on - strand, within Dsui_3518 at 3782.269 kb on - strand, within Dsui_3518 at 3782.325 kb on + strand, within Dsui_3518 at 3782.333 kb on - strand, within Dsui_3518 at 3782.333 kb on - strand, within Dsui_3518 at 3782.369 kb on - strand, within Dsui_3518 at 3782.393 kb on + strand, within Dsui_3518 at 3782.401 kb on - strand, within Dsui_3518 at 3782.404 kb on + strand, within Dsui_3518 at 3782.465 kb on - strand, within Dsui_3518 at 3782.525 kb on + strand at 3782.525 kb on + strand at 3782.526 kb on - strand at 3782.605 kb on + strand at 3782.605 kb on + strand at 3782.630 kb on + strand at 3782.662 kb on + strand at 3782.670 kb on - strand at 3782.671 kb on - strand at 3782.731 kb on - strand at 3782.831 kb on + strand, within Dsui_3519 at 3782.839 kb on + strand, within Dsui_3519 at 3782.839 kb on - strand, within Dsui_3519 at 3782.847 kb on - strand, within Dsui_3519 at 3782.944 kb on - strand, within Dsui_3519 at 3782.976 kb on - strand, within Dsui_3519 at 3783.007 kb on + strand, within Dsui_3519 at 3783.178 kb on + strand, within Dsui_3519 at 3783.193 kb on - strand, within Dsui_3519 at 3783.217 kb on + strand, within Dsui_3519 at 3783.302 kb on - strand, within Dsui_3519 at 3783.332 kb on - strand, within Dsui_3519 at 3783.384 kb on - strand, within Dsui_3519 at 3783.514 kb on + strand, within Dsui_3519 at 3783.522 kb on + strand, within Dsui_3519
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 3,781,179 + +1.0 3,781,187 - -1.2 3,781,207 + -0.2 3,781,221 - -0.8 3,781,315 + +0.7 3,781,327 - -0.5 3,781,371 + Dsui_3517 0.15 -1.6 3,781,379 - Dsui_3517 0.16 -1.6 3,781,386 - Dsui_3517 0.17 -1.2 3,781,575 + Dsui_3517 0.40 +0.7 3,781,605 + Dsui_3517 0.43 +0.7 3,781,605 + Dsui_3517 0.43 +0.7 3,781,643 + Dsui_3517 0.48 -0.3 3,781,728 - Dsui_3517 0.58 -0.9 3,781,750 + Dsui_3517 0.61 -0.3 3,781,773 + Dsui_3517 0.63 -0.9 3,781,773 + Dsui_3517 0.63 +0.7 3,781,820 + Dsui_3517 0.69 -0.9 3,781,823 - Dsui_3517 0.69 +0.2 3,781,829 - Dsui_3517 0.70 +0.7 3,781,879 + +0.7 3,781,880 + +0.7 3,781,880 + Dsui_3517 0.76 +0.7 3,781,880 + +0.7 3,781,880 + Dsui_3517 0.76 -0.3 3,781,888 - Dsui_3517 0.77 +1.0 3,781,888 - Dsui_3517 0.77 -0.4 3,781,888 - Dsui_3517 0.77 +0.3 3,781,888 - Dsui_3517 0.77 -0.9 3,781,965 + Dsui_3517 0.87 -0.9 3,781,973 + Dsui_3517 0.87 +0.7 3,781,973 + Dsui_3517 0.87 -0.9 3,781,981 - Dsui_3517 0.88 +0.4 3,781,981 - Dsui_3517 0.88 -0.7 3,782,015 - +0.7 3,782,065 - -0.6 3,782,065 - +0.1 3,782,065 - +0.7 3,782,109 + -2.7 3,782,109 + -0.3 3,782,122 - -1.6 3,782,171 + +0.6 3,782,232 - Dsui_3518 0.20 +0.4 3,782,269 - Dsui_3518 0.30 +1.8 3,782,325 + Dsui_3518 0.45 +1.6 3,782,333 - Dsui_3518 0.47 -0.3 3,782,333 - Dsui_3518 0.47 -0.3 3,782,369 - Dsui_3518 0.57 +0.8 3,782,393 + Dsui_3518 0.64 +0.8 3,782,401 - Dsui_3518 0.66 +0.4 3,782,404 + Dsui_3518 0.67 +1.1 3,782,465 - Dsui_3518 0.84 +1.4 3,782,525 + +0.4 3,782,525 + +0.4 3,782,526 - -0.1 3,782,605 + -0.3 3,782,605 + +0.7 3,782,630 + -0.3 3,782,662 + -0.9 3,782,670 - -0.1 3,782,671 - +0.1 3,782,731 - -0.3 3,782,831 + Dsui_3519 0.15 -1.6 3,782,839 + Dsui_3519 0.16 -0.3 3,782,839 - Dsui_3519 0.16 -0.2 3,782,847 - Dsui_3519 0.16 +0.2 3,782,944 - Dsui_3519 0.23 -0.3 3,782,976 - Dsui_3519 0.25 -0.2 3,783,007 + Dsui_3519 0.27 +0.7 3,783,178 + Dsui_3519 0.38 -0.3 3,783,193 - Dsui_3519 0.39 +0.5 3,783,217 + Dsui_3519 0.40 -2.7 3,783,302 - Dsui_3519 0.46 -0.4 3,783,332 - Dsui_3519 0.48 -0.2 3,783,384 - Dsui_3519 0.51 -0.5 3,783,514 + Dsui_3519 0.60 -1.3 3,783,522 + Dsui_3519 0.60 -1.3
Or see this region's nucleotide sequence