Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3436 and Dsui_3437 overlap by 4 nucleotides Dsui_3437 and Dsui_3438 are separated by 13 nucleotides
Dsui_3436: Dsui_3436 - beta-hydroxyacid dehydrogenase, 3-hydroxyisobutyrate dehydrogenase, at 3,689,338 to 3,690,228
_3436
Dsui_3437: Dsui_3437 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 3,690,225 to 3,693,257
_3437
Dsui_3438: Dsui_3438 - beta-hydroxyacid dehydrogenase, 3-hydroxyisobutyrate dehydrogenase, at 3,693,271 to 3,694,170
_3438
Position (kb)
3690
3691
3692
3693
3694 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3689.282 kb on - strand at 3689.311 kb on + strand at 3689.372 kb on - strand at 3689.400 kb on - strand at 3689.433 kb on - strand, within Dsui_3436 at 3689.600 kb on - strand, within Dsui_3436 at 3689.838 kb on - strand, within Dsui_3436 at 3690.306 kb on + strand at 3690.364 kb on - strand at 3690.364 kb on - strand at 3690.458 kb on + strand at 3690.573 kb on + strand, within Dsui_3437 at 3690.681 kb on + strand, within Dsui_3437 at 3690.687 kb on + strand, within Dsui_3437 at 3690.739 kb on + strand at 3690.747 kb on - strand, within Dsui_3437 at 3690.837 kb on - strand, within Dsui_3437 at 3690.925 kb on + strand, within Dsui_3437 at 3691.018 kb on - strand, within Dsui_3437 at 3691.018 kb on - strand, within Dsui_3437 at 3691.149 kb on + strand, within Dsui_3437 at 3691.239 kb on + strand, within Dsui_3437 at 3691.378 kb on + strand, within Dsui_3437 at 3691.378 kb on + strand, within Dsui_3437 at 3691.406 kb on - strand, within Dsui_3437 at 3691.479 kb on - strand, within Dsui_3437 at 3691.514 kb on - strand, within Dsui_3437 at 3691.677 kb on + strand, within Dsui_3437 at 3691.683 kb on - strand, within Dsui_3437 at 3691.720 kb on + strand, within Dsui_3437 at 3691.847 kb on + strand, within Dsui_3437 at 3692.409 kb on - strand, within Dsui_3437 at 3692.487 kb on + strand at 3692.526 kb on - strand, within Dsui_3437 at 3692.545 kb on - strand, within Dsui_3437 at 3692.605 kb on - strand, within Dsui_3437 at 3692.669 kb on - strand, within Dsui_3437 at 3692.700 kb on - strand, within Dsui_3437 at 3692.736 kb on + strand at 3692.807 kb on - strand, within Dsui_3437 at 3692.870 kb on - strand, within Dsui_3437 at 3692.883 kb on - strand, within Dsui_3437 at 3693.188 kb on - strand at 3693.411 kb on + strand at 3693.541 kb on + strand, within Dsui_3438 at 3693.549 kb on - strand, within Dsui_3438 at 3693.618 kb on + strand at 3693.883 kb on - strand, within Dsui_3438 at 3693.943 kb on + strand, within Dsui_3438 at 3693.981 kb on + strand, within Dsui_3438 at 3694.106 kb on + strand at 3694.124 kb on - strand at 3694.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 3,689,282 - +0.1 3,689,311 + -2.9 3,689,372 - -0.4 3,689,400 - -0.8 3,689,433 - Dsui_3436 0.11 +1.1 3,689,600 - Dsui_3436 0.29 +0.2 3,689,838 - Dsui_3436 0.56 +0.2 3,690,306 + -0.3 3,690,364 - -0.3 3,690,364 - -2.2 3,690,458 + -1.9 3,690,573 + Dsui_3437 0.11 -2.7 3,690,681 + Dsui_3437 0.15 -1.6 3,690,687 + Dsui_3437 0.15 +0.7 3,690,739 + +0.7 3,690,747 - Dsui_3437 0.17 -0.1 3,690,837 - Dsui_3437 0.20 +0.0 3,690,925 + Dsui_3437 0.23 -1.6 3,691,018 - Dsui_3437 0.26 +0.5 3,691,018 - Dsui_3437 0.26 -2.6 3,691,149 + Dsui_3437 0.30 +0.7 3,691,239 + Dsui_3437 0.33 -0.9 3,691,378 + Dsui_3437 0.38 -1.6 3,691,378 + Dsui_3437 0.38 -2.1 3,691,406 - Dsui_3437 0.39 +0.7 3,691,479 - Dsui_3437 0.41 +0.7 3,691,514 - Dsui_3437 0.42 -0.5 3,691,677 + Dsui_3437 0.48 -0.3 3,691,683 - Dsui_3437 0.48 +0.1 3,691,720 + Dsui_3437 0.49 +0.7 3,691,847 + Dsui_3437 0.53 -2.1 3,692,409 - Dsui_3437 0.72 +0.0 3,692,487 + +0.7 3,692,526 - Dsui_3437 0.76 -0.4 3,692,545 - Dsui_3437 0.76 +0.3 3,692,605 - Dsui_3437 0.78 +0.2 3,692,669 - Dsui_3437 0.81 +0.4 3,692,700 - Dsui_3437 0.82 +0.6 3,692,736 + +0.7 3,692,807 - Dsui_3437 0.85 +0.0 3,692,870 - Dsui_3437 0.87 -0.7 3,692,883 - Dsui_3437 0.88 -0.7 3,693,188 - -0.1 3,693,411 + +0.7 3,693,541 + Dsui_3438 0.30 -2.3 3,693,549 - Dsui_3438 0.31 -0.1 3,693,618 + +0.7 3,693,883 - Dsui_3438 0.68 -0.1 3,693,943 + Dsui_3438 0.75 -0.9 3,693,981 + Dsui_3438 0.79 -2.1 3,694,106 + +0.7 3,694,124 - -0.0 3,694,151 - -0.1
Or see this region's nucleotide sequence