Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3211 and Dsui_3212 are separated by 69 nucleotides Dsui_3212 and Dsui_3213 are separated by 330 nucleotides Dsui_3213 and Dsui_3214 are separated by 125 nucleotides
Dsui_3211: Dsui_3211 - glutamate dehydrogenase/leucine dehydrogenase, at 3,445,273 to 3,446,619
_3211
Dsui_3212: Dsui_3212 - acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, at 3,446,689 to 3,448,356
_3212
Dsui_3213: Dsui_3213 - CBS domain-containing protein, at 3,448,687 to 3,449,136
_3213
Dsui_3214: Dsui_3214 - ATP-dependent exonuclase V beta subunit, helicase and exonuclease domain-containing, at 3,449,262 to 3,452,603
_3214
Position (kb)
3446
3447
3448
3449 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3445.797 kb on - strand at 3445.889 kb on - strand at 3445.983 kb on - strand, within Dsui_3211 at 3446.075 kb on + strand, within Dsui_3211 at 3446.206 kb on - strand, within Dsui_3211 at 3446.261 kb on + strand, within Dsui_3211 at 3446.329 kb on - strand, within Dsui_3211 at 3446.384 kb on + strand, within Dsui_3211 at 3446.429 kb on + strand, within Dsui_3211 at 3446.464 kb on - strand, within Dsui_3211 at 3446.775 kb on + strand at 3446.798 kb on - strand at 3446.807 kb on + strand at 3446.817 kb on + strand at 3446.837 kb on + strand at 3446.892 kb on - strand, within Dsui_3212 at 3446.979 kb on - strand, within Dsui_3212 at 3447.062 kb on + strand at 3447.070 kb on - strand, within Dsui_3212 at 3447.096 kb on + strand, within Dsui_3212 at 3447.142 kb on + strand, within Dsui_3212 at 3447.171 kb on + strand at 3447.237 kb on + strand, within Dsui_3212 at 3447.245 kb on + strand, within Dsui_3212 at 3447.246 kb on + strand, within Dsui_3212 at 3447.358 kb on + strand at 3447.366 kb on - strand, within Dsui_3212 at 3447.366 kb on - strand, within Dsui_3212 at 3447.433 kb on + strand at 3447.438 kb on + strand, within Dsui_3212 at 3447.474 kb on - strand, within Dsui_3212 at 3447.486 kb on - strand, within Dsui_3212 at 3447.540 kb on + strand, within Dsui_3212 at 3447.549 kb on - strand, within Dsui_3212 at 3447.559 kb on - strand, within Dsui_3212 at 3447.561 kb on + strand, within Dsui_3212 at 3447.634 kb on + strand at 3447.637 kb on - strand, within Dsui_3212 at 3447.668 kb on + strand, within Dsui_3212 at 3447.770 kb on + strand at 3447.783 kb on - strand, within Dsui_3212 at 3447.799 kb on - strand, within Dsui_3212 at 3447.852 kb on + strand, within Dsui_3212 at 3447.860 kb on - strand, within Dsui_3212 at 3447.937 kb on + strand, within Dsui_3212 at 3447.945 kb on - strand, within Dsui_3212 at 3447.948 kb on + strand, within Dsui_3212 at 3447.956 kb on - strand, within Dsui_3212 at 3448.039 kb on - strand, within Dsui_3212 at 3448.056 kb on - strand, within Dsui_3212 at 3448.061 kb on - strand, within Dsui_3212 at 3448.084 kb on - strand, within Dsui_3212 at 3448.102 kb on - strand, within Dsui_3212 at 3448.120 kb on - strand, within Dsui_3212 at 3448.144 kb on - strand, within Dsui_3212 at 3448.202 kb on + strand at 3448.252 kb on - strand at 3448.254 kb on + strand at 3448.256 kb on + strand at 3448.259 kb on - strand at 3448.264 kb on - strand at 3448.366 kb on - strand at 3448.421 kb on - strand at 3448.472 kb on + strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.603 kb on - strand at 3448.690 kb on + strand at 3448.809 kb on - strand, within Dsui_3213 at 3448.900 kb on - strand, within Dsui_3213 at 3449.065 kb on + strand at 3449.065 kb on + strand at 3449.087 kb on + strand at 3449.095 kb on - strand at 3449.095 kb on - strand at 3449.095 kb on - strand at 3449.095 kb on - strand at 3449.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 3,445,797 - -0.8 3,445,889 - +0.8 3,445,983 - Dsui_3211 0.53 -0.2 3,446,075 + Dsui_3211 0.60 -0.3 3,446,206 - Dsui_3211 0.69 -0.2 3,446,261 + Dsui_3211 0.73 -0.0 3,446,329 - Dsui_3211 0.78 +0.8 3,446,384 + Dsui_3211 0.82 +0.2 3,446,429 + Dsui_3211 0.86 -0.1 3,446,464 - Dsui_3211 0.88 +0.8 3,446,775 + -1.6 3,446,798 - +1.9 3,446,807 + +0.8 3,446,817 + -0.8 3,446,837 + +0.8 3,446,892 - Dsui_3212 0.12 +0.9 3,446,979 - Dsui_3212 0.17 +0.8 3,447,062 + +0.8 3,447,070 - Dsui_3212 0.23 +0.3 3,447,096 + Dsui_3212 0.24 -1.6 3,447,142 + Dsui_3212 0.27 -0.8 3,447,171 + -0.2 3,447,237 + Dsui_3212 0.33 -0.8 3,447,245 + Dsui_3212 0.33 -0.8 3,447,246 + Dsui_3212 0.33 -1.6 3,447,358 + +0.8 3,447,366 - Dsui_3212 0.41 +0.3 3,447,366 - Dsui_3212 0.41 +1.7 3,447,433 + +0.8 3,447,438 + Dsui_3212 0.45 -0.2 3,447,474 - Dsui_3212 0.47 +0.7 3,447,486 - Dsui_3212 0.48 +0.4 3,447,540 + Dsui_3212 0.51 -0.2 3,447,549 - Dsui_3212 0.52 +0.2 3,447,559 - Dsui_3212 0.52 +1.2 3,447,561 + Dsui_3212 0.52 -0.8 3,447,634 + -0.2 3,447,637 - Dsui_3212 0.57 +1.4 3,447,668 + Dsui_3212 0.59 -1.6 3,447,770 + -0.2 3,447,783 - Dsui_3212 0.66 +1.7 3,447,799 - Dsui_3212 0.67 +0.5 3,447,852 + Dsui_3212 0.70 -0.8 3,447,860 - Dsui_3212 0.70 +0.5 3,447,937 + Dsui_3212 0.75 -0.2 3,447,945 - Dsui_3212 0.75 +0.6 3,447,948 + Dsui_3212 0.75 -2.8 3,447,956 - Dsui_3212 0.76 +0.7 3,448,039 - Dsui_3212 0.81 +0.6 3,448,056 - Dsui_3212 0.82 +0.5 3,448,061 - Dsui_3212 0.82 +0.3 3,448,084 - Dsui_3212 0.84 +0.1 3,448,102 - Dsui_3212 0.85 +0.1 3,448,120 - Dsui_3212 0.86 +1.2 3,448,144 - Dsui_3212 0.87 +0.4 3,448,202 + -0.2 3,448,252 - +0.6 3,448,254 + -1.2 3,448,256 + -0.8 3,448,259 - +0.9 3,448,264 - +0.8 3,448,366 - +0.0 3,448,421 - -0.1 3,448,472 + -0.2 3,448,480 - -0.2 3,448,480 - -0.2 3,448,480 - +0.8 3,448,480 - +0.8 3,448,480 - +0.8 3,448,603 - -0.2 3,448,690 + -1.2 3,448,809 - Dsui_3213 0.27 +1.3 3,448,900 - Dsui_3213 0.47 +0.5 3,449,065 + +0.8 3,449,065 + -0.2 3,449,087 + -1.2 3,449,095 - +0.5 3,449,095 - +0.3 3,449,095 - +0.7 3,449,095 - +0.4 3,449,183 - +0.0
Or see this region's nucleotide sequence