Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3128 and Dsui_3129 are separated by 40 nucleotides Dsui_3129 and Dsui_3130 are separated by 244 nucleotides Dsui_3130 and Dsui_3131 are separated by 269 nucleotides
Dsui_3128: Dsui_3128 - ribonuclease R, at 3,350,134 to 3,352,620
_3128
Dsui_3129: Dsui_3129 - tRNA-Leu, at 3,352,661 to 3,352,747
_3129
Dsui_3130: Dsui_3130 - tRNA-Leu, at 3,352,992 to 3,353,078
_3130
Dsui_3131: Dsui_3131 - adenylosuccinate synthase, at 3,353,348 to 3,354,658
_3131
Position (kb)
3352
3353
3354 Strain fitness (log2 ratio)
-1
0
1
2 at 3352.022 kb on + strand, within Dsui_3128 at 3352.030 kb on - strand, within Dsui_3128 at 3352.070 kb on - strand, within Dsui_3128 at 3352.130 kb on - strand, within Dsui_3128 at 3352.151 kb on - strand, within Dsui_3128 at 3352.201 kb on + strand at 3352.257 kb on + strand, within Dsui_3128 at 3352.324 kb on + strand, within Dsui_3128 at 3352.325 kb on + strand, within Dsui_3128 at 3352.331 kb on + strand, within Dsui_3128 at 3352.332 kb on - strand, within Dsui_3128 at 3352.332 kb on - strand, within Dsui_3128 at 3352.415 kb on - strand at 3352.438 kb on + strand at 3352.958 kb on - strand at 3353.061 kb on + strand, within Dsui_3130 at 3353.232 kb on + strand at 3353.269 kb on + strand at 3353.269 kb on + strand at 3353.277 kb on - strand at 3353.277 kb on - strand at 3353.277 kb on - strand at 3353.339 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 3,352,022 + Dsui_3128 0.76 +0.8 3,352,030 - Dsui_3128 0.76 +0.8 3,352,070 - Dsui_3128 0.78 +0.8 3,352,130 - Dsui_3128 0.80 +2.3 3,352,151 - Dsui_3128 0.81 +1.8 3,352,201 + +0.8 3,352,257 + Dsui_3128 0.85 +0.8 3,352,324 + Dsui_3128 0.88 -0.8 3,352,325 + Dsui_3128 0.88 -0.0 3,352,331 + Dsui_3128 0.88 -0.8 3,352,332 - Dsui_3128 0.88 +1.9 3,352,332 - Dsui_3128 0.88 +0.5 3,352,415 - +1.5 3,352,438 + -1.6 3,352,958 - +0.8 3,353,061 + Dsui_3130 0.79 +0.7 3,353,232 + -0.4 3,353,269 + +0.5 3,353,269 + +0.4 3,353,277 - -0.5 3,353,277 - -0.8 3,353,277 - -0.5 3,353,339 - -0.2
Or see this region's nucleotide sequence