Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3125 and Dsui_3126 overlap by 4 nucleotides Dsui_3126 and Dsui_3127 are separated by 201 nucleotides Dsui_3127 and Dsui_3128 are separated by 16 nucleotides
Dsui_3125: Dsui_3125 - cytochrome c, mono- and diheme variants family, at 3,346,411 to 3,348,009
_3125
Dsui_3126: Dsui_3126 - heme d1 biosynthesis radical SAM protein NirJ, at 3,348,006 to 3,349,169
_3126
Dsui_3127: Dsui_3127 - 23S RNA-specific pseudouridylate synthase, at 3,349,371 to 3,350,117
_3127
Dsui_3128: Dsui_3128 - ribonuclease R, at 3,350,134 to 3,352,620
_3128
Position (kb)
3348
3349
3350 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3347.108 kb on - strand, within Dsui_3125 at 3347.120 kb on + strand, within Dsui_3125 at 3347.140 kb on - strand, within Dsui_3125 at 3347.193 kb on + strand at 3347.265 kb on + strand, within Dsui_3125 at 3347.274 kb on - strand, within Dsui_3125 at 3347.382 kb on + strand, within Dsui_3125 at 3347.492 kb on + strand, within Dsui_3125 at 3347.765 kb on + strand, within Dsui_3125 at 3347.772 kb on - strand, within Dsui_3125 at 3347.773 kb on - strand, within Dsui_3125 at 3347.991 kb on - strand at 3348.188 kb on + strand at 3348.190 kb on - strand, within Dsui_3126 at 3348.252 kb on + strand, within Dsui_3126 at 3348.269 kb on + strand at 3348.317 kb on + strand, within Dsui_3126 at 3348.381 kb on - strand, within Dsui_3126 at 3348.400 kb on - strand, within Dsui_3126 at 3348.418 kb on - strand, within Dsui_3126 at 3348.461 kb on - strand, within Dsui_3126 at 3348.521 kb on + strand at 3348.599 kb on + strand at 3348.604 kb on + strand, within Dsui_3126 at 3348.607 kb on - strand at 3348.612 kb on - strand, within Dsui_3126 at 3348.668 kb on + strand, within Dsui_3126 at 3348.761 kb on + strand at 3348.769 kb on - strand, within Dsui_3126 at 3348.853 kb on - strand, within Dsui_3126 at 3348.918 kb on - strand, within Dsui_3126 at 3348.923 kb on - strand, within Dsui_3126 at 3348.969 kb on - strand, within Dsui_3126 at 3348.987 kb on + strand, within Dsui_3126 at 3349.054 kb on - strand at 3349.058 kb on + strand at 3349.066 kb on - strand at 3349.085 kb on - strand at 3349.177 kb on + strand at 3349.234 kb on + strand at 3349.234 kb on + strand at 3349.245 kb on - strand at 3349.252 kb on - strand at 3349.336 kb on - strand at 3349.341 kb on + strand at 3349.616 kb on + strand, within Dsui_3127 at 3349.621 kb on + strand, within Dsui_3127 at 3349.778 kb on - strand at 3350.016 kb on - strand, within Dsui_3127 at 3350.065 kb on - strand at 3350.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 3,347,108 - Dsui_3125 0.44 +0.8 3,347,120 + Dsui_3125 0.44 -2.9 3,347,140 - Dsui_3125 0.46 +0.8 3,347,193 + +0.8 3,347,265 + Dsui_3125 0.53 -1.2 3,347,274 - Dsui_3125 0.54 -0.8 3,347,382 + Dsui_3125 0.61 -1.2 3,347,492 + Dsui_3125 0.68 -0.2 3,347,765 + Dsui_3125 0.85 -2.2 3,347,772 - Dsui_3125 0.85 +0.8 3,347,773 - Dsui_3125 0.85 -1.6 3,347,991 - -0.8 3,348,188 + +1.8 3,348,190 - Dsui_3126 0.16 +0.8 3,348,252 + Dsui_3126 0.21 -1.2 3,348,269 + +0.8 3,348,317 + Dsui_3126 0.27 -0.8 3,348,381 - Dsui_3126 0.32 -0.8 3,348,400 - Dsui_3126 0.34 -0.2 3,348,418 - Dsui_3126 0.35 -0.2 3,348,461 - Dsui_3126 0.39 -2.2 3,348,521 + +0.8 3,348,599 + +0.8 3,348,604 + Dsui_3126 0.51 +0.8 3,348,607 - +0.8 3,348,612 - Dsui_3126 0.52 -0.2 3,348,668 + Dsui_3126 0.57 -0.2 3,348,761 + -0.2 3,348,769 - Dsui_3126 0.66 -1.6 3,348,853 - Dsui_3126 0.73 +0.8 3,348,918 - Dsui_3126 0.78 +0.8 3,348,923 - Dsui_3126 0.79 -0.2 3,348,969 - Dsui_3126 0.83 -1.2 3,348,987 + Dsui_3126 0.84 -0.8 3,349,054 - -2.4 3,349,058 + -0.8 3,349,066 - -1.2 3,349,085 - -1.8 3,349,177 + -0.2 3,349,234 + +0.8 3,349,234 + -1.2 3,349,245 - -0.2 3,349,252 - -0.2 3,349,336 - -2.0 3,349,341 + -2.9 3,349,616 + Dsui_3127 0.33 +0.1 3,349,621 + Dsui_3127 0.33 -0.8 3,349,778 - +0.8 3,350,016 - Dsui_3127 0.86 -2.7 3,350,065 - -1.6 3,350,080 - -0.2
Or see this region's nucleotide sequence