Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2994 and Dsui_2995 are separated by 126 nucleotides Dsui_2995 and Dsui_2996 are separated by 144 nucleotides Dsui_2996 and Dsui_2997 are separated by 124 nucleotides Dsui_2997 and Dsui_2998 are separated by 225 nucleotides
Dsui_2994: Dsui_2994 - fructose-2,6-bisphosphatase, at 3,203,912 to 3,204,613
_2994
Dsui_2995: Dsui_2995 - hypothetical protein, at 3,204,740 to 3,205,330
_2995
Dsui_2996: Dsui_2996 - type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 3,205,475 to 3,207,289
_2996
Dsui_2997: Dsui_2997 - hypothetical protein, at 3,207,414 to 3,207,710
_2997
Dsui_2998: Dsui_2998 - ABC-type phosphate/phosphonate transport system, periplasmic component, at 3,207,936 to 3,208,802
_2998
Position (kb)
3205
3206
3207
3208 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3204.605 kb on - strand at 3204.639 kb on - strand at 3204.769 kb on + strand at 3204.797 kb on + strand at 3204.979 kb on - strand, within Dsui_2995 at 3204.988 kb on - strand, within Dsui_2995 at 3205.071 kb on - strand, within Dsui_2995 at 3205.081 kb on - strand, within Dsui_2995 at 3205.086 kb on - strand, within Dsui_2995 at 3205.228 kb on + strand at 3205.236 kb on - strand, within Dsui_2995 at 3205.302 kb on + strand at 3205.307 kb on + strand at 3205.384 kb on + strand at 3205.521 kb on - strand at 3205.677 kb on - strand, within Dsui_2996 at 3205.702 kb on + strand at 3205.727 kb on + strand, within Dsui_2996 at 3205.732 kb on + strand, within Dsui_2996 at 3205.735 kb on - strand, within Dsui_2996 at 3205.767 kb on - strand, within Dsui_2996 at 3205.767 kb on - strand, within Dsui_2996 at 3205.821 kb on + strand, within Dsui_2996 at 3205.830 kb on - strand, within Dsui_2996 at 3205.994 kb on + strand, within Dsui_2996 at 3206.085 kb on - strand, within Dsui_2996 at 3206.124 kb on - strand, within Dsui_2996 at 3206.153 kb on - strand, within Dsui_2996 at 3206.256 kb on + strand, within Dsui_2996 at 3206.418 kb on + strand, within Dsui_2996 at 3206.497 kb on - strand, within Dsui_2996 at 3206.519 kb on + strand at 3206.527 kb on - strand, within Dsui_2996 at 3206.690 kb on - strand, within Dsui_2996 at 3206.712 kb on - strand, within Dsui_2996 at 3206.712 kb on - strand, within Dsui_2996 at 3206.777 kb on - strand, within Dsui_2996 at 3206.796 kb on - strand, within Dsui_2996 at 3206.842 kb on + strand, within Dsui_2996 at 3206.880 kb on + strand at 3206.960 kb on - strand, within Dsui_2996 at 3207.087 kb on - strand, within Dsui_2996 at 3207.108 kb on - strand at 3207.158 kb on - strand at 3207.315 kb on + strand at 3207.484 kb on - strand at 3207.520 kb on + strand, within Dsui_2997 at 3207.584 kb on - strand, within Dsui_2997 at 3207.624 kb on + strand, within Dsui_2997 at 3207.679 kb on - strand, within Dsui_2997 at 3207.968 kb on + strand at 3207.978 kb on + strand at 3208.055 kb on + strand, within Dsui_2998 at 3208.055 kb on + strand, within Dsui_2998 at 3208.055 kb on + strand, within Dsui_2998 at 3208.063 kb on - strand at 3208.063 kb on - strand at 3208.199 kb on + strand, within Dsui_2998 at 3208.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 3,204,605 - -0.8 3,204,639 - +0.8 3,204,769 + +0.8 3,204,797 + +0.8 3,204,979 - Dsui_2995 0.40 +0.8 3,204,988 - Dsui_2995 0.42 -0.8 3,205,071 - Dsui_2995 0.56 -0.2 3,205,081 - Dsui_2995 0.58 +0.8 3,205,086 - Dsui_2995 0.59 -0.2 3,205,228 + -1.2 3,205,236 - Dsui_2995 0.84 -1.2 3,205,302 + +0.8 3,205,307 + -0.2 3,205,384 + -1.8 3,205,521 - -0.2 3,205,677 - Dsui_2996 0.11 +1.1 3,205,702 + -0.2 3,205,727 + Dsui_2996 0.14 -0.8 3,205,732 + Dsui_2996 0.14 -0.2 3,205,735 - Dsui_2996 0.14 +0.7 3,205,767 - Dsui_2996 0.16 +0.6 3,205,767 - Dsui_2996 0.16 +0.4 3,205,821 + Dsui_2996 0.19 -1.2 3,205,830 - Dsui_2996 0.20 +3.1 3,205,994 + Dsui_2996 0.29 +0.8 3,206,085 - Dsui_2996 0.34 -0.2 3,206,124 - Dsui_2996 0.36 +0.2 3,206,153 - Dsui_2996 0.37 +1.8 3,206,256 + Dsui_2996 0.43 +0.8 3,206,418 + Dsui_2996 0.52 +0.8 3,206,497 - Dsui_2996 0.56 +2.1 3,206,519 + -0.2 3,206,527 - Dsui_2996 0.58 -0.6 3,206,690 - Dsui_2996 0.67 +0.0 3,206,712 - Dsui_2996 0.68 -0.0 3,206,712 - Dsui_2996 0.68 +1.0 3,206,777 - Dsui_2996 0.72 -0.2 3,206,796 - Dsui_2996 0.73 +0.6 3,206,842 + Dsui_2996 0.75 +0.8 3,206,880 + +0.8 3,206,960 - Dsui_2996 0.82 +1.4 3,207,087 - Dsui_2996 0.89 -1.2 3,207,108 - -1.1 3,207,158 - -2.0 3,207,315 + -0.8 3,207,484 - +0.8 3,207,520 + Dsui_2997 0.36 -0.5 3,207,584 - Dsui_2997 0.57 +0.8 3,207,624 + Dsui_2997 0.71 -0.8 3,207,679 - Dsui_2997 0.89 -0.8 3,207,968 + -1.7 3,207,978 + +1.2 3,208,055 + Dsui_2998 0.14 +0.8 3,208,055 + Dsui_2998 0.14 -0.2 3,208,055 + Dsui_2998 0.14 +0.8 3,208,063 - -0.8 3,208,063 - +0.8 3,208,199 + Dsui_2998 0.30 -0.2 3,208,248 - +0.8
Or see this region's nucleotide sequence