Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2944 and Dsui_2945 are separated by 313 nucleotides Dsui_2945 and Dsui_2946 overlap by 1 nucleotides Dsui_2946 and Dsui_2947 overlap by 4 nucleotides Dsui_2947 and Dsui_2948 are separated by 170 nucleotides
Dsui_2944: Dsui_2944 - diguanylate cyclase (GGDEF) domain-containing protein, at 3,149,972 to 3,151,684
_2944
Dsui_2945: Dsui_2945 - hypothetical protein, at 3,151,998 to 3,152,486
_2945
Dsui_2946: Dsui_2946 - DNA/RNA helicase, superfamily II, at 3,152,486 to 3,153,916
_2946
Dsui_2947: Dsui_2947 - putative metal-dependent hydrolase, at 3,153,913 to 3,154,479
_2947
Dsui_2948: Dsui_2948 - RNA polymerase sigma factor, sigma-70 family, at 3,154,650 to 3,155,147
_2948
Position (kb)
3152
3153
3154 Strain fitness (log2 ratio)
-1
0
1 at 3151.557 kb on + strand at 3151.617 kb on - strand at 3151.629 kb on - strand at 3151.786 kb on - strand at 3151.811 kb on + strand at 3151.870 kb on - strand at 3151.907 kb on - strand at 3151.950 kb on - strand at 3151.955 kb on + strand at 3151.963 kb on + strand at 3151.975 kb on + strand at 3151.983 kb on - strand at 3152.125 kb on + strand, within Dsui_2945 at 3152.174 kb on - strand at 3152.347 kb on - strand at 3152.353 kb on + strand, within Dsui_2945 at 3152.372 kb on + strand, within Dsui_2945 at 3152.407 kb on + strand, within Dsui_2945 at 3152.408 kb on + strand, within Dsui_2945 at 3152.408 kb on + strand, within Dsui_2945 at 3152.437 kb on - strand at 3152.658 kb on + strand, within Dsui_2946 at 3152.712 kb on + strand, within Dsui_2946 at 3152.712 kb on + strand, within Dsui_2946 at 3152.730 kb on - strand at 3152.767 kb on + strand, within Dsui_2946 at 3152.778 kb on + strand, within Dsui_2946 at 3152.810 kb on - strand at 3152.961 kb on + strand, within Dsui_2946 at 3152.965 kb on + strand, within Dsui_2946 at 3152.993 kb on - strand at 3153.070 kb on + strand, within Dsui_2946 at 3153.308 kb on - strand, within Dsui_2946 at 3153.308 kb on - strand at 3153.308 kb on - strand at 3153.563 kb on - strand, within Dsui_2946 at 3153.693 kb on + strand, within Dsui_2946 at 3153.819 kb on + strand at 3153.882 kb on - strand at 3153.981 kb on - strand, within Dsui_2947 at 3154.021 kb on + strand, within Dsui_2947 at 3154.079 kb on + strand, within Dsui_2947 at 3154.195 kb on - strand, within Dsui_2947 at 3154.233 kb on + strand, within Dsui_2947 at 3154.282 kb on + strand, within Dsui_2947 at 3154.315 kb on + strand, within Dsui_2947 at 3154.323 kb on - strand, within Dsui_2947 at 3154.394 kb on - strand at 3154.540 kb on - strand at 3154.540 kb on - strand at 3154.601 kb on + strand at 3154.609 kb on - strand at 3154.653 kb on + strand at 3154.690 kb on - strand at 3154.690 kb on - strand at 3154.690 kb on - strand at 3154.824 kb on - strand, within Dsui_2948 at 3154.859 kb on - strand at 3154.889 kb on - strand, within Dsui_2948 at 3154.894 kb on - strand, within Dsui_2948 at 3154.899 kb on + strand, within Dsui_2948
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 3,151,557 + -0.2 3,151,617 - +0.8 3,151,629 - +0.8 3,151,786 - +0.8 3,151,811 + -0.2 3,151,870 - +0.8 3,151,907 - -0.2 3,151,950 - -0.2 3,151,955 + -0.2 3,151,963 + +0.3 3,151,975 + +0.5 3,151,983 - -0.7 3,152,125 + Dsui_2945 0.26 +0.1 3,152,174 - +0.8 3,152,347 - -0.2 3,152,353 + Dsui_2945 0.73 +0.1 3,152,372 + Dsui_2945 0.76 -0.0 3,152,407 + Dsui_2945 0.84 -0.1 3,152,408 + Dsui_2945 0.84 -0.0 3,152,408 + Dsui_2945 0.84 +0.1 3,152,437 - +0.8 3,152,658 + Dsui_2946 0.12 +0.3 3,152,712 + Dsui_2946 0.16 +1.3 3,152,712 + Dsui_2946 0.16 +0.0 3,152,730 - +0.8 3,152,767 + Dsui_2946 0.20 -0.1 3,152,778 + Dsui_2946 0.20 +0.1 3,152,810 - +0.8 3,152,961 + Dsui_2946 0.33 +0.2 3,152,965 + Dsui_2946 0.33 +0.5 3,152,993 - +0.8 3,153,070 + Dsui_2946 0.41 +0.3 3,153,308 - Dsui_2946 0.57 +0.8 3,153,308 - +0.8 3,153,308 - -0.2 3,153,563 - Dsui_2946 0.75 -0.7 3,153,693 + Dsui_2946 0.84 +0.6 3,153,819 + +0.8 3,153,882 - +0.8 3,153,981 - Dsui_2947 0.12 +0.8 3,154,021 + Dsui_2947 0.19 +1.0 3,154,079 + Dsui_2947 0.29 +0.1 3,154,195 - Dsui_2947 0.50 +0.8 3,154,233 + Dsui_2947 0.56 +0.8 3,154,282 + Dsui_2947 0.65 -1.5 3,154,315 + Dsui_2947 0.71 -1.5 3,154,323 - Dsui_2947 0.72 -0.7 3,154,394 - -0.2 3,154,540 - +0.8 3,154,540 - +0.8 3,154,601 + +0.2 3,154,609 - -0.2 3,154,653 + +0.3 3,154,690 - +0.8 3,154,690 - +0.8 3,154,690 - +0.8 3,154,824 - Dsui_2948 0.35 -0.2 3,154,859 - +0.8 3,154,889 - Dsui_2948 0.48 -0.2 3,154,894 - Dsui_2948 0.49 -1.2 3,154,899 + Dsui_2948 0.50 +0.7
Or see this region's nucleotide sequence