Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2789 and Dsui_2790 are separated by 66 nucleotides Dsui_2790 and Dsui_2791 are separated by 23 nucleotides Dsui_2791 and Dsui_2792 are separated by 89 nucleotides Dsui_2792 and Dsui_2793 are separated by 232 nucleotides Dsui_2793 and Dsui_2794 are separated by 23 nucleotides Dsui_2794 and Dsui_2795 are separated by 55 nucleotides Dsui_2795 and Dsui_2796 are separated by 77 nucleotides
Dsui_2789: Dsui_2789 - glutamyl-tRNA synthetase, at 2,961,691 to 2,963,082
_2789
Dsui_2790: Dsui_2790 - tRNA-Ala, at 2,963,149 to 2,963,224
_2790
Dsui_2791: Dsui_2791 - tRNA-Glu, at 2,963,248 to 2,963,323
_2791
Dsui_2792: Dsui_2792 - tRNA-Asp, at 2,963,413 to 2,963,489
_2792
Dsui_2793: Dsui_2793 - tRNA-Ala, at 2,963,722 to 2,963,797
_2793
Dsui_2794: Dsui_2794 - tRNA-Glu, at 2,963,821 to 2,963,896
_2794
Dsui_2795: Dsui_2795 - tRNA-Asp, at 2,963,952 to 2,964,028
_2795
Dsui_2796: Dsui_2796 - NAD-dependent protein deacetylase, SIR2 family, at 2,964,106 to 2,964,867
_2796
Position (kb)
2963
2964 Strain fitness (log2 ratio)
-1
0
1 at 2963.099 kb on + strand at 2963.107 kb on - strand at 2963.323 kb on + strand at 2963.360 kb on + strand at 2963.385 kb on + strand at 2963.550 kb on + strand at 2963.550 kb on + strand at 2963.582 kb on + strand at 2963.583 kb on + strand at 2963.631 kb on + strand at 2963.636 kb on + strand at 2963.937 kb on + strand at 2963.937 kb on + strand at 2964.153 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,963,099 + -0.1 2,963,107 - +0.9 2,963,323 + +0.4 2,963,360 + -0.0 2,963,385 + -0.3 2,963,550 + -0.8 2,963,550 + -0.7 2,963,582 + -0.4 2,963,583 + -1.7 2,963,631 + -0.1 2,963,636 + +0.9 2,963,937 + -0.1 2,963,937 + -0.3 2,964,153 + +0.8
Or see this region's nucleotide sequence