Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2499 and Dsui_2500 are separated by 52 nucleotides Dsui_2500 and Dsui_2501 are separated by 112 nucleotides Dsui_2501 and Dsui_2502 overlap by 4 nucleotides Dsui_2502 and Dsui_2503 are separated by 2 nucleotides
Dsui_2499: Dsui_2499 - MerE protein, at 2,647,150 to 2,647,386
_2499
Dsui_2500: Dsui_2500 - site-specific recombinase, DNA invertase Pin, at 2,647,439 to 2,648,053
_2500
Dsui_2501: Dsui_2501 - TniQ protein, at 2,648,166 to 2,649,332
_2501
Dsui_2502: Dsui_2502 - TniB protein, at 2,649,329 to 2,650,237
_2502
Dsui_2503: Dsui_2503 - Mu transposase/integrase, at 2,650,240 to 2,651,922
_2503
Position (kb)
2648
2649
2650 Strain fitness (log2 ratio)
-2
-1
0
1 at 2647.222 kb on + strand, within Dsui_2499 at 2647.227 kb on + strand, within Dsui_2499 at 2647.235 kb on - strand, within Dsui_2499 at 2647.299 kb on + strand, within Dsui_2499 at 2647.307 kb on - strand at 2647.324 kb on - strand at 2647.474 kb on - strand at 2647.692 kb on + strand, within Dsui_2500 at 2647.748 kb on + strand, within Dsui_2500 at 2647.769 kb on + strand, within Dsui_2500 at 2647.777 kb on - strand, within Dsui_2500 at 2647.798 kb on + strand at 2647.800 kb on + strand at 2647.823 kb on - strand, within Dsui_2500 at 2648.008 kb on + strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.231 kb on + strand at 2648.239 kb on - strand at 2648.352 kb on + strand, within Dsui_2501 at 2648.419 kb on + strand, within Dsui_2501 at 2648.495 kb on + strand, within Dsui_2501 at 2648.643 kb on - strand at 2648.643 kb on - strand, within Dsui_2501 at 2648.646 kb on + strand, within Dsui_2501 at 2649.070 kb on - strand, within Dsui_2501 at 2649.070 kb on - strand at 2649.364 kb on + strand at 2649.566 kb on + strand, within Dsui_2502 at 2649.703 kb on + strand, within Dsui_2502 at 2649.711 kb on - strand, within Dsui_2502 at 2649.824 kb on - strand, within Dsui_2502 at 2650.079 kb on + strand, within Dsui_2502 at 2650.216 kb on - strand at 2650.238 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,647,222 + Dsui_2499 0.30 +0.8 2,647,227 + Dsui_2499 0.32 -0.8 2,647,235 - Dsui_2499 0.36 +0.8 2,647,299 + Dsui_2499 0.63 +0.8 2,647,307 - +0.8 2,647,324 - +0.8 2,647,474 - +0.3 2,647,692 + Dsui_2500 0.41 -0.8 2,647,748 + Dsui_2500 0.50 +0.8 2,647,769 + Dsui_2500 0.54 +0.8 2,647,777 - Dsui_2500 0.55 -0.4 2,647,798 + +0.8 2,647,800 + +0.8 2,647,823 - Dsui_2500 0.62 -0.4 2,648,008 + -0.8 2,648,016 - +0.5 2,648,016 - -0.5 2,648,016 - -0.4 2,648,016 - -0.3 2,648,016 - -0.0 2,648,231 + -1.2 2,648,239 - +0.8 2,648,352 + Dsui_2501 0.16 +0.8 2,648,419 + Dsui_2501 0.22 -0.8 2,648,495 + Dsui_2501 0.28 +0.8 2,648,643 - +0.8 2,648,643 - Dsui_2501 0.41 +0.8 2,648,646 + Dsui_2501 0.41 -0.2 2,649,070 - Dsui_2501 0.77 -0.2 2,649,070 - +0.8 2,649,364 + +0.8 2,649,566 + Dsui_2502 0.26 -0.8 2,649,703 + Dsui_2502 0.41 +0.8 2,649,711 - Dsui_2502 0.42 +0.8 2,649,824 - Dsui_2502 0.54 -0.2 2,650,079 + Dsui_2502 0.83 +0.8 2,650,216 - +0.8 2,650,238 + -2.7
Or see this region's nucleotide sequence