Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2403 and Dsui_2404 are separated by 3 nucleotides Dsui_2404 and Dsui_2405 overlap by 4 nucleotides Dsui_2405 and Dsui_2406 are separated by 199 nucleotides Dsui_2406 and Dsui_2407 are separated by 12 nucleotides
Dsui_2403: Dsui_2403 - universal bacterial protein YeaZ, at 2,555,662 to 2,556,345
_2403
Dsui_2404: Dsui_2404 - ribosomal-protein-alanine acetyltransferase, at 2,556,349 to 2,556,840
_2404
Dsui_2405: Dsui_2405 - uracil-DNA glycosylase, family 4, at 2,556,837 to 2,557,694
_2405
Dsui_2406: Dsui_2406 - hypothetical protein, at 2,557,894 to 2,558,496
_2406
Dsui_2407: Dsui_2407 - beta-propeller domain-containing protein, methanol dehydrogenase, at 2,558,509 to 2,559,339
_2407
Position (kb)
2556
2557
2558 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2556.354 kb on + strand at 2556.452 kb on + strand, within Dsui_2404 at 2556.517 kb on + strand, within Dsui_2404 at 2556.709 kb on + strand, within Dsui_2404 at 2556.715 kb on + strand, within Dsui_2404 at 2556.842 kb on + strand at 2557.699 kb on + strand at 2557.896 kb on - strand at 2557.896 kb on - strand at 2557.938 kb on + strand at 2557.941 kb on - strand at 2557.946 kb on - strand at 2557.976 kb on - strand at 2557.983 kb on + strand, within Dsui_2406 at 2557.983 kb on + strand, within Dsui_2406 at 2557.983 kb on + strand, within Dsui_2406 at 2557.983 kb on + strand, within Dsui_2406 at 2557.991 kb on - strand at 2557.991 kb on - strand at 2557.991 kb on - strand, within Dsui_2406 at 2558.064 kb on + strand, within Dsui_2406 at 2558.067 kb on - strand at 2558.072 kb on - strand at 2558.177 kb on + strand, within Dsui_2406 at 2558.195 kb on - strand at 2558.238 kb on + strand, within Dsui_2406 at 2558.243 kb on + strand, within Dsui_2406 at 2558.288 kb on + strand, within Dsui_2406 at 2558.420 kb on - strand, within Dsui_2406 at 2558.445 kb on + strand at 2558.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,556,354 + -0.2 2,556,452 + Dsui_2404 0.21 -3.1 2,556,517 + Dsui_2404 0.34 -3.3 2,556,709 + Dsui_2404 0.73 -1.5 2,556,715 + Dsui_2404 0.74 -4.1 2,556,842 + -0.3 2,557,699 + -1.2 2,557,896 - +0.8 2,557,896 - -0.2 2,557,938 + +1.8 2,557,941 - -0.2 2,557,946 - +0.8 2,557,976 - -0.2 2,557,983 + Dsui_2406 0.15 +2.5 2,557,983 + Dsui_2406 0.15 +0.2 2,557,983 + Dsui_2406 0.15 -0.2 2,557,983 + Dsui_2406 0.15 -1.0 2,557,991 - +0.8 2,557,991 - -2.0 2,557,991 - Dsui_2406 0.16 +0.8 2,558,064 + Dsui_2406 0.28 +0.2 2,558,067 - -1.2 2,558,072 - -0.2 2,558,177 + Dsui_2406 0.47 -0.2 2,558,195 - -0.8 2,558,238 + Dsui_2406 0.57 -1.5 2,558,243 + Dsui_2406 0.58 -1.1 2,558,288 + Dsui_2406 0.65 -0.2 2,558,420 - Dsui_2406 0.87 -1.2 2,558,445 + -0.5 2,558,448 + -1.4
Or see this region's nucleotide sequence