Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2389 and Dsui_2390 are separated by 101 nucleotides Dsui_2390 and Dsui_2391 overlap by 4 nucleotides Dsui_2391 and Dsui_2392 are separated by 2 nucleotides
Dsui_2389: Dsui_2389 - putative membrane protein, at 2,541,439 to 2,542,710
_2389
Dsui_2390: Dsui_2390 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 2,542,812 to 2,543,528
_2390
Dsui_2391: Dsui_2391 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 2,543,525 to 2,544,013
_2391
Dsui_2392: Dsui_2392 - 2''-5'' RNA ligase, at 2,544,016 to 2,544,591
_2392
Position (kb)
2542
2543
2544 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2541.931 kb on - strand at 2541.960 kb on + strand at 2541.997 kb on - strand, within Dsui_2389 at 2542.019 kb on + strand at 2542.034 kb on + strand at 2542.092 kb on - strand, within Dsui_2389 at 2542.149 kb on - strand, within Dsui_2389 at 2542.181 kb on - strand, within Dsui_2389 at 2542.218 kb on - strand, within Dsui_2389 at 2542.345 kb on - strand, within Dsui_2389 at 2542.374 kb on + strand, within Dsui_2389 at 2542.430 kb on - strand at 2542.579 kb on - strand, within Dsui_2389 at 2542.583 kb on - strand at 2542.583 kb on - strand at 2542.629 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.652 kb on - strand at 2542.786 kb on - strand at 2544.017 kb on + strand at 2544.162 kb on - strand at 2544.192 kb on + strand, within Dsui_2392 at 2544.221 kb on - strand at 2544.255 kb on - strand at 2544.255 kb on - strand at 2544.315 kb on + strand, within Dsui_2392 at 2544.524 kb on + strand, within Dsui_2392
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,541,931 - +0.8 2,541,960 + +0.8 2,541,997 - Dsui_2389 0.44 -1.5 2,542,019 + +0.8 2,542,034 + -0.2 2,542,092 - Dsui_2389 0.51 +0.8 2,542,149 - Dsui_2389 0.56 -0.2 2,542,181 - Dsui_2389 0.58 +0.8 2,542,218 - Dsui_2389 0.61 -1.2 2,542,345 - Dsui_2389 0.71 +1.8 2,542,374 + Dsui_2389 0.74 -0.2 2,542,430 - +0.8 2,542,579 - Dsui_2389 0.90 -2.9 2,542,583 - +0.8 2,542,583 - -0.2 2,542,629 - +0.8 2,542,652 - -1.5 2,542,652 - +0.8 2,542,652 - +0.8 2,542,652 - -0.2 2,542,652 - -1.5 2,542,652 - +0.8 2,542,652 - +0.8 2,542,786 - +0.8 2,544,017 + -0.4 2,544,162 - -0.2 2,544,192 + Dsui_2392 0.31 +0.1 2,544,221 - +0.8 2,544,255 - +0.8 2,544,255 - -0.2 2,544,315 + Dsui_2392 0.52 -0.2 2,544,524 + Dsui_2392 0.88 -1.0
Or see this region's nucleotide sequence