Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2331 and Dsui_2332 are separated by 38 nucleotides Dsui_2332 and Dsui_2333 are separated by 10 nucleotides Dsui_2333 and Dsui_2334 are separated by 151 nucleotides
Dsui_2331: Dsui_2331 - heat shock protein, at 2,484,492 to 2,485,643
_2331
Dsui_2332: Dsui_2332 - uracil-xanthine permease, at 2,485,682 to 2,486,923
_2332
Dsui_2333: Dsui_2333 - uracil phosphoribosyltransferase, at 2,486,934 to 2,487,560
_2333
Dsui_2334: Dsui_2334 - tRNA-dihydrouridine synthase, at 2,487,712 to 2,488,674
_2334
Position (kb)
2485
2486
2487 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2484.996 kb on - strand, within Dsui_2331 at 2485.093 kb on + strand at 2485.167 kb on - strand, within Dsui_2331 at 2485.209 kb on + strand, within Dsui_2331 at 2485.243 kb on - strand, within Dsui_2331 at 2485.402 kb on - strand, within Dsui_2331 at 2485.425 kb on - strand, within Dsui_2331 at 2485.728 kb on - strand at 2485.764 kb on - strand at 2485.827 kb on - strand, within Dsui_2332 at 2485.853 kb on + strand, within Dsui_2332 at 2485.883 kb on + strand, within Dsui_2332 at 2485.891 kb on - strand, within Dsui_2332 at 2485.965 kb on - strand, within Dsui_2332 at 2486.116 kb on + strand, within Dsui_2332 at 2486.124 kb on - strand, within Dsui_2332 at 2486.127 kb on + strand at 2486.149 kb on + strand, within Dsui_2332 at 2486.162 kb on + strand at 2486.170 kb on - strand, within Dsui_2332 at 2486.170 kb on - strand, within Dsui_2332 at 2486.171 kb on - strand, within Dsui_2332 at 2486.218 kb on + strand at 2486.368 kb on - strand, within Dsui_2332 at 2486.533 kb on + strand at 2486.533 kb on + strand, within Dsui_2332 at 2486.536 kb on - strand, within Dsui_2332 at 2486.537 kb on - strand, within Dsui_2332 at 2486.541 kb on - strand, within Dsui_2332 at 2486.541 kb on - strand, within Dsui_2332 at 2486.554 kb on - strand, within Dsui_2332 at 2486.671 kb on - strand, within Dsui_2332 at 2486.680 kb on + strand at 2486.801 kb on - strand at 2486.803 kb on + strand at 2486.811 kb on - strand at 2486.903 kb on - strand at 2486.908 kb on - strand at 2486.975 kb on - strand at 2486.983 kb on + strand at 2486.983 kb on + strand at 2486.983 kb on + strand at 2486.991 kb on - strand at 2486.991 kb on - strand at 2487.076 kb on - strand, within Dsui_2333 at 2487.112 kb on + strand at 2487.197 kb on + strand, within Dsui_2333 at 2487.229 kb on + strand at 2487.248 kb on - strand, within Dsui_2333 at 2487.259 kb on + strand at 2487.450 kb on - strand, within Dsui_2333 at 2487.620 kb on - strand at 2487.749 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,484,996 - Dsui_2331 0.44 +0.8 2,485,093 + -1.2 2,485,167 - Dsui_2331 0.59 -0.2 2,485,209 + Dsui_2331 0.62 +0.8 2,485,243 - Dsui_2331 0.65 -0.5 2,485,402 - Dsui_2331 0.79 -1.5 2,485,425 - Dsui_2331 0.81 -0.2 2,485,728 - +0.4 2,485,764 - +0.6 2,485,827 - Dsui_2332 0.12 -0.8 2,485,853 + Dsui_2332 0.14 -1.2 2,485,883 + Dsui_2332 0.16 -1.6 2,485,891 - Dsui_2332 0.17 +0.1 2,485,965 - Dsui_2332 0.23 -2.9 2,486,116 + Dsui_2332 0.35 -0.2 2,486,124 - Dsui_2332 0.36 -0.1 2,486,127 + -0.2 2,486,149 + Dsui_2332 0.38 -1.2 2,486,162 + +0.8 2,486,170 - Dsui_2332 0.39 -0.5 2,486,170 - Dsui_2332 0.39 +0.9 2,486,171 - Dsui_2332 0.39 +1.1 2,486,218 + +0.8 2,486,368 - Dsui_2332 0.55 -0.4 2,486,533 + -0.2 2,486,533 + Dsui_2332 0.69 -2.4 2,486,536 - Dsui_2332 0.69 -1.7 2,486,537 - Dsui_2332 0.69 +0.1 2,486,541 - Dsui_2332 0.69 -0.2 2,486,541 - Dsui_2332 0.69 -0.3 2,486,554 - Dsui_2332 0.70 -1.0 2,486,671 - Dsui_2332 0.80 +0.8 2,486,680 + +0.8 2,486,801 - +1.8 2,486,803 + +0.8 2,486,811 - -1.8 2,486,903 - -0.3 2,486,908 - -2.8 2,486,975 - -0.9 2,486,983 + +0.8 2,486,983 + +0.8 2,486,983 + -0.2 2,486,991 - -0.2 2,486,991 - +0.8 2,487,076 - Dsui_2333 0.23 +0.3 2,487,112 + -0.2 2,487,197 + Dsui_2333 0.42 -0.2 2,487,229 + +0.8 2,487,248 - Dsui_2333 0.50 -0.9 2,487,259 + -0.2 2,487,450 - Dsui_2333 0.82 -2.8 2,487,620 - -0.2 2,487,749 - +0.8
Or see this region's nucleotide sequence