Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2195 and Dsui_2196 are separated by 125 nucleotides Dsui_2196 and Dsui_2197 are separated by 15 nucleotides Dsui_2197 and Dsui_2198 are separated by 114 nucleotides
Dsui_2195: Dsui_2195 - trigger factor, at 2,330,596 to 2,331,900
_2195
Dsui_2196: Dsui_2196 - ATP-dependent Clp protease, proteolytic subunit ClpP, at 2,332,026 to 2,332,667
_2196
Dsui_2197: Dsui_2197 - endopeptidase Clp ATP-binding regulatory subunit ClpX, at 2,332,683 to 2,333,957
_2197
Dsui_2198: Dsui_2198 - ATP-dependent protease La, at 2,334,072 to 2,336,489
_2198
Position (kb)
2332
2333
2334 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2331.684 kb on + strand, within Dsui_2195 at 2331.697 kb on - strand at 2331.699 kb on + strand, within Dsui_2195 at 2331.699 kb on + strand, within Dsui_2195 at 2331.751 kb on + strand, within Dsui_2195 at 2331.808 kb on + strand at 2331.982 kb on + strand at 2331.990 kb on + strand at 2331.990 kb on + strand at 2331.990 kb on - strand at 2331.997 kb on - strand at 2332.062 kb on + strand at 2332.064 kb on - strand at 2332.070 kb on - strand at 2332.071 kb on - strand at 2332.143 kb on - strand, within Dsui_2196 at 2332.146 kb on + strand, within Dsui_2196 at 2332.158 kb on - strand, within Dsui_2196 at 2332.183 kb on + strand, within Dsui_2196 at 2332.385 kb on + strand, within Dsui_2196 at 2332.608 kb on + strand at 2332.629 kb on + strand at 2332.629 kb on + strand at 2332.637 kb on - strand at 2332.639 kb on + strand at 2332.639 kb on + strand at 2332.726 kb on - strand at 2332.750 kb on - strand at 2332.876 kb on - strand, within Dsui_2197 at 2332.903 kb on + strand, within Dsui_2197 at 2332.959 kb on + strand, within Dsui_2197 at 2332.967 kb on - strand, within Dsui_2197 at 2333.032 kb on - strand, within Dsui_2197 at 2333.037 kb on - strand, within Dsui_2197 at 2333.052 kb on - strand at 2333.080 kb on + strand, within Dsui_2197 at 2333.087 kb on - strand, within Dsui_2197 at 2333.122 kb on - strand, within Dsui_2197 at 2333.132 kb on + strand, within Dsui_2197 at 2333.281 kb on - strand, within Dsui_2197 at 2333.321 kb on + strand, within Dsui_2197 at 2333.321 kb on + strand, within Dsui_2197 at 2333.321 kb on + strand, within Dsui_2197 at 2333.328 kb on - strand, within Dsui_2197 at 2333.357 kb on + strand, within Dsui_2197 at 2333.446 kb on + strand, within Dsui_2197 at 2333.505 kb on - strand at 2333.510 kb on + strand, within Dsui_2197 at 2333.541 kb on + strand, within Dsui_2197 at 2333.541 kb on + strand, within Dsui_2197 at 2333.560 kb on + strand, within Dsui_2197 at 2333.636 kb on + strand, within Dsui_2197 at 2333.644 kb on - strand, within Dsui_2197 at 2333.695 kb on + strand, within Dsui_2197 at 2333.705 kb on - strand, within Dsui_2197 at 2333.718 kb on - strand, within Dsui_2197 at 2333.825 kb on + strand, within Dsui_2197 at 2333.833 kb on + strand at 2333.918 kb on + strand at 2333.918 kb on + strand at 2333.931 kb on - strand at 2333.933 kb on + strand at 2333.933 kb on + strand at 2334.002 kb on + strand at 2334.038 kb on + strand at 2334.038 kb on + strand at 2334.046 kb on - strand at 2334.071 kb on - strand at 2334.079 kb on + strand at 2334.108 kb on + strand at 2334.108 kb on + strand at 2334.628 kb on + strand, within Dsui_2198 at 2334.745 kb on + strand, within Dsui_2198 at 2334.755 kb on + strand, within Dsui_2198 at 2334.779 kb on + strand, within Dsui_2198 at 2334.779 kb on + strand, within Dsui_2198 at 2334.779 kb on + strand, within Dsui_2198 at 2334.779 kb on + strand, within Dsui_2198 at 2334.787 kb on - strand at 2334.908 kb on + strand, within Dsui_2198
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,331,684 + Dsui_2195 0.83 -3.9 2,331,697 - +0.9 2,331,699 + Dsui_2195 0.85 -2.8 2,331,699 + Dsui_2195 0.85 -4.5 2,331,751 + Dsui_2195 0.89 -4.4 2,331,808 + -4.7 2,331,982 + +0.5 2,331,990 + -0.1 2,331,990 + +0.1 2,331,990 - -0.7 2,331,997 - -1.1 2,332,062 + +1.8 2,332,064 - -0.1 2,332,070 - -0.7 2,332,071 - -0.1 2,332,143 - Dsui_2196 0.18 -1.1 2,332,146 + Dsui_2196 0.19 +3.2 2,332,158 - Dsui_2196 0.21 +0.9 2,332,183 + Dsui_2196 0.24 +2.1 2,332,385 + Dsui_2196 0.56 +2.7 2,332,608 + +1.7 2,332,629 + +1.8 2,332,629 + +2.7 2,332,637 - +0.9 2,332,639 + +0.2 2,332,639 + +0.7 2,332,726 - +0.9 2,332,750 - -0.1 2,332,876 - Dsui_2197 0.15 -0.1 2,332,903 + Dsui_2197 0.17 +1.4 2,332,959 + Dsui_2197 0.22 +1.5 2,332,967 - Dsui_2197 0.22 -0.1 2,333,032 - Dsui_2197 0.27 +0.9 2,333,037 - Dsui_2197 0.28 -1.5 2,333,052 - +0.9 2,333,080 + Dsui_2197 0.31 +1.2 2,333,087 - Dsui_2197 0.32 -1.5 2,333,122 - Dsui_2197 0.34 +0.9 2,333,132 + Dsui_2197 0.35 +0.8 2,333,281 - Dsui_2197 0.47 +0.9 2,333,321 + Dsui_2197 0.50 +1.4 2,333,321 + Dsui_2197 0.50 +1.2 2,333,321 + Dsui_2197 0.50 +1.2 2,333,328 - Dsui_2197 0.51 +0.9 2,333,357 + Dsui_2197 0.53 +1.1 2,333,446 + Dsui_2197 0.60 +1.3 2,333,505 - +0.9 2,333,510 + Dsui_2197 0.65 +1.2 2,333,541 + Dsui_2197 0.67 +1.1 2,333,541 + Dsui_2197 0.67 +1.0 2,333,560 + Dsui_2197 0.69 +1.6 2,333,636 + Dsui_2197 0.75 +1.2 2,333,644 - Dsui_2197 0.75 -0.1 2,333,695 + Dsui_2197 0.79 +1.2 2,333,705 - Dsui_2197 0.80 +0.9 2,333,718 - Dsui_2197 0.81 +0.9 2,333,825 + Dsui_2197 0.90 +1.0 2,333,833 + +0.8 2,333,918 + +0.6 2,333,918 + +0.3 2,333,931 - -2.1 2,333,933 + +0.7 2,333,933 + +0.6 2,334,002 + +0.4 2,334,038 + +1.8 2,334,038 + +1.2 2,334,046 - -0.1 2,334,071 - -0.7 2,334,079 + +1.0 2,334,108 + +1.5 2,334,108 + +2.4 2,334,628 + Dsui_2198 0.23 +1.4 2,334,745 + Dsui_2198 0.28 +1.9 2,334,755 + Dsui_2198 0.28 +2.0 2,334,779 + Dsui_2198 0.29 +1.5 2,334,779 + Dsui_2198 0.29 +1.7 2,334,779 + Dsui_2198 0.29 +1.5 2,334,779 + Dsui_2198 0.29 +1.6 2,334,787 - +0.9 2,334,908 + Dsui_2198 0.35 +1.4
Or see this region's nucleotide sequence