Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2193 and Dsui_2194 are separated by 131 nucleotides Dsui_2194 and Dsui_2195 are separated by 46 nucleotides Dsui_2195 and Dsui_2196 are separated by 125 nucleotides Dsui_2196 and Dsui_2197 are separated by 15 nucleotides
Dsui_2193: Dsui_2193 - squalene synthase HpnC, at 2,329,509 to 2,330,333
_2193
Dsui_2194: Dsui_2194 - tRNA-Leu, at 2,330,465 to 2,330,549
_2194
Dsui_2195: Dsui_2195 - trigger factor, at 2,330,596 to 2,331,900
_2195
Dsui_2196: Dsui_2196 - ATP-dependent Clp protease, proteolytic subunit ClpP, at 2,332,026 to 2,332,667
_2196
Dsui_2197: Dsui_2197 - endopeptidase Clp ATP-binding regulatory subunit ClpX, at 2,332,683 to 2,333,957
_2197
Position (kb)
2330
2331
2332 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 2329.600 kb on + strand at 2329.753 kb on + strand, within Dsui_2193 at 2329.761 kb on - strand, within Dsui_2193 at 2329.761 kb on - strand, within Dsui_2193 at 2329.761 kb on - strand, within Dsui_2193 at 2329.780 kb on - strand, within Dsui_2193 at 2329.780 kb on - strand, within Dsui_2193 at 2329.780 kb on - strand, within Dsui_2193 at 2329.780 kb on - strand, within Dsui_2193 at 2329.856 kb on + strand at 2329.859 kb on - strand, within Dsui_2193 at 2329.859 kb on - strand, within Dsui_2193 at 2329.927 kb on - strand, within Dsui_2193 at 2329.990 kb on + strand at 2330.013 kb on - strand, within Dsui_2193 at 2330.209 kb on - strand, within Dsui_2193 at 2330.213 kb on - strand, within Dsui_2193 at 2330.266 kb on - strand at 2330.337 kb on - strand at 2330.368 kb on + strand at 2330.423 kb on + strand at 2330.423 kb on - strand at 2330.430 kb on - strand at 2330.673 kb on + strand at 2330.743 kb on + strand, within Dsui_2195 at 2330.755 kb on + strand, within Dsui_2195 at 2330.763 kb on + strand, within Dsui_2195 at 2330.803 kb on + strand, within Dsui_2195 at 2330.836 kb on + strand, within Dsui_2195 at 2330.883 kb on + strand, within Dsui_2195 at 2330.918 kb on + strand, within Dsui_2195 at 2331.069 kb on + strand, within Dsui_2195 at 2331.152 kb on + strand, within Dsui_2195 at 2331.275 kb on - strand at 2331.357 kb on + strand, within Dsui_2195 at 2331.427 kb on + strand, within Dsui_2195 at 2331.444 kb on + strand, within Dsui_2195 at 2331.477 kb on + strand, within Dsui_2195 at 2331.485 kb on - strand at 2331.485 kb on - strand, within Dsui_2195 at 2331.684 kb on + strand, within Dsui_2195 at 2331.697 kb on - strand at 2331.699 kb on + strand, within Dsui_2195 at 2331.699 kb on + strand, within Dsui_2195 at 2331.751 kb on + strand, within Dsui_2195 at 2331.808 kb on + strand at 2331.982 kb on + strand at 2331.990 kb on + strand at 2331.990 kb on + strand at 2331.990 kb on - strand at 2331.997 kb on - strand at 2332.062 kb on + strand at 2332.064 kb on - strand at 2332.070 kb on - strand at 2332.071 kb on - strand at 2332.143 kb on - strand, within Dsui_2196 at 2332.146 kb on + strand, within Dsui_2196 at 2332.158 kb on - strand, within Dsui_2196 at 2332.183 kb on + strand, within Dsui_2196 at 2332.385 kb on + strand, within Dsui_2196 at 2332.608 kb on + strand at 2332.629 kb on + strand at 2332.629 kb on + strand at 2332.637 kb on - strand at 2332.639 kb on + strand at 2332.639 kb on + strand at 2332.726 kb on - strand at 2332.750 kb on - strand at 2332.876 kb on - strand, within Dsui_2197
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,329,600 + +0.9 2,329,753 + Dsui_2193 0.30 -0.1 2,329,761 - Dsui_2193 0.31 +3.4 2,329,761 - Dsui_2193 0.31 +1.7 2,329,761 - Dsui_2193 0.31 +1.3 2,329,780 - Dsui_2193 0.33 +1.6 2,329,780 - Dsui_2193 0.33 +2.8 2,329,780 - Dsui_2193 0.33 +2.3 2,329,780 - Dsui_2193 0.33 +0.9 2,329,856 + +0.9 2,329,859 - Dsui_2193 0.42 +0.9 2,329,859 - Dsui_2193 0.42 +1.6 2,329,927 - Dsui_2193 0.51 +1.9 2,329,990 + -0.1 2,330,013 - Dsui_2193 0.61 +0.9 2,330,209 - Dsui_2193 0.85 +1.9 2,330,213 - Dsui_2193 0.85 +0.7 2,330,266 - +2.0 2,330,337 - -0.1 2,330,368 + -0.7 2,330,423 + -0.7 2,330,423 - -0.5 2,330,430 - +0.7 2,330,673 + -2.2 2,330,743 + Dsui_2195 0.11 -4.0 2,330,755 + Dsui_2195 0.12 -3.9 2,330,763 + Dsui_2195 0.13 -2.8 2,330,803 + Dsui_2195 0.16 -5.5 2,330,836 + Dsui_2195 0.18 -6.3 2,330,883 + Dsui_2195 0.22 -3.7 2,330,918 + Dsui_2195 0.25 -3.1 2,331,069 + Dsui_2195 0.36 -4.5 2,331,152 + Dsui_2195 0.43 -2.7 2,331,275 - +0.9 2,331,357 + Dsui_2195 0.58 -4.0 2,331,427 + Dsui_2195 0.64 -3.1 2,331,444 + Dsui_2195 0.65 -4.7 2,331,477 + Dsui_2195 0.68 -3.8 2,331,485 - +0.9 2,331,485 - Dsui_2195 0.68 -1.1 2,331,684 + Dsui_2195 0.83 -3.9 2,331,697 - +0.9 2,331,699 + Dsui_2195 0.85 -2.8 2,331,699 + Dsui_2195 0.85 -4.5 2,331,751 + Dsui_2195 0.89 -4.4 2,331,808 + -4.7 2,331,982 + +0.5 2,331,990 + -0.1 2,331,990 + +0.1 2,331,990 - -0.7 2,331,997 - -1.1 2,332,062 + +1.8 2,332,064 - -0.1 2,332,070 - -0.7 2,332,071 - -0.1 2,332,143 - Dsui_2196 0.18 -1.1 2,332,146 + Dsui_2196 0.19 +3.2 2,332,158 - Dsui_2196 0.21 +0.9 2,332,183 + Dsui_2196 0.24 +2.1 2,332,385 + Dsui_2196 0.56 +2.7 2,332,608 + +1.7 2,332,629 + +1.8 2,332,629 + +2.7 2,332,637 - +0.9 2,332,639 + +0.2 2,332,639 + +0.7 2,332,726 - +0.9 2,332,750 - -0.1 2,332,876 - Dsui_2197 0.15 -0.1
Or see this region's nucleotide sequence