Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2097 and Dsui_2098 are separated by 77 nucleotides Dsui_2098 and Dsui_2099 are separated by 13 nucleotides Dsui_2099 and Dsui_2100 are separated by 294 nucleotides
Dsui_2097: Dsui_2097 - putative phosphohydrolase, at 2,222,198 to 2,223,391
_2097
Dsui_2098: Dsui_2098 - hypothetical protein, at 2,223,469 to 2,223,855
_2098
Dsui_2099: Dsui_2099 - RNA polymerase sigma factor, sigma-70 family, at 2,223,869 to 2,224,462
_2099
Dsui_2100: Dsui_2100 - hypothetical protein, at 2,224,757 to 2,225,161
_2100
Position (kb)
2223
2224 Strain fitness (log2 ratio)
-1
0
1 at 2222.504 kb on + strand, within Dsui_2097 at 2222.629 kb on + strand at 2222.634 kb on + strand, within Dsui_2097 at 2222.642 kb on - strand at 2222.710 kb on - strand, within Dsui_2097 at 2222.928 kb on + strand at 2222.935 kb on - strand, within Dsui_2097 at 2223.325 kb on + strand at 2223.497 kb on + strand at 2223.497 kb on + strand at 2223.671 kb on - strand, within Dsui_2098 at 2223.872 kb on + strand at 2223.875 kb on - strand at 2223.880 kb on - strand at 2223.880 kb on - strand at 2223.977 kb on - strand at 2223.979 kb on + strand at 2223.987 kb on - strand at 2223.987 kb on - strand at 2224.169 kb on + strand, within Dsui_2099 at 2224.178 kb on - strand at 2224.178 kb on - strand, within Dsui_2099 at 2224.269 kb on + strand at 2224.311 kb on - strand at 2224.353 kb on - strand, within Dsui_2099 at 2224.410 kb on + strand at 2224.467 kb on + strand at 2224.484 kb on - strand at 2224.484 kb on - strand at 2224.623 kb on + strand at 2224.631 kb on - strand at 2224.631 kb on - strand at 2224.664 kb on - strand at 2224.664 kb on - strand at 2224.715 kb on - strand at 2224.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,222,504 + Dsui_2097 0.26 -0.0 2,222,629 + +1.0 2,222,634 + Dsui_2097 0.37 -1.0 2,222,642 - -0.0 2,222,710 - Dsui_2097 0.43 -0.6 2,222,928 + +1.0 2,222,935 - Dsui_2097 0.62 +1.0 2,223,325 + -1.3 2,223,497 + -0.6 2,223,497 + -0.0 2,223,671 - Dsui_2098 0.52 +1.1 2,223,872 + +1.0 2,223,875 - -0.6 2,223,880 - +1.0 2,223,880 - -1.0 2,223,977 - +1.0 2,223,979 + +1.0 2,223,987 - +1.0 2,223,987 - -1.0 2,224,169 + Dsui_2099 0.51 +1.0 2,224,178 - +1.0 2,224,178 - Dsui_2099 0.52 -1.0 2,224,269 + +1.0 2,224,311 - -0.6 2,224,353 - Dsui_2099 0.81 -0.0 2,224,410 + +1.0 2,224,467 + +1.0 2,224,484 - -1.0 2,224,484 - -0.0 2,224,623 + +1.0 2,224,631 - +0.3 2,224,631 - +0.6 2,224,664 - -1.6 2,224,664 - -0.6 2,224,715 - +1.0 2,224,720 + +1.0
Or see this region's nucleotide sequence