Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2053 and Dsui_2054 are separated by 16 nucleotides Dsui_2054 and Dsui_2055 are separated by 194 nucleotides Dsui_2055 and Dsui_2056 are separated by 674 nucleotides
Dsui_2053: Dsui_2053 - HNH endonuclease, at 2,173,150 to 2,173,530
_2053
Dsui_2054: Dsui_2054 - hypothetical protein, at 2,173,547 to 2,173,915
_2054
Dsui_2055: Dsui_2055 - methylase involved in ubiquinone/menaquinone biosynthesis, at 2,174,110 to 2,174,913
_2055
Dsui_2056: Dsui_2056 - hypothetical protein, at 2,175,588 to 2,176,142
_2056
Position (kb)
2174
2175 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2173.147 kb on + strand at 2173.153 kb on + strand at 2173.160 kb on + strand at 2173.168 kb on - strand at 2173.181 kb on + strand at 2173.243 kb on + strand, within Dsui_2053 at 2173.359 kb on - strand, within Dsui_2053 at 2173.448 kb on + strand, within Dsui_2053 at 2173.503 kb on - strand at 2173.503 kb on - strand at 2173.525 kb on + strand at 2173.592 kb on + strand at 2173.624 kb on + strand, within Dsui_2054 at 2173.714 kb on - strand, within Dsui_2054 at 2173.778 kb on + strand, within Dsui_2054 at 2173.809 kb on - strand, within Dsui_2054 at 2173.941 kb on - strand at 2174.022 kb on - strand at 2174.058 kb on + strand at 2174.089 kb on - strand at 2174.099 kb on - strand at 2174.182 kb on + strand at 2174.189 kb on + strand at 2174.262 kb on - strand at 2174.271 kb on - strand at 2174.401 kb on - strand at 2174.654 kb on - strand, within Dsui_2055 at 2174.775 kb on - strand at 2174.823 kb on - strand, within Dsui_2055 at 2174.922 kb on - strand at 2174.927 kb on - strand at 2174.953 kb on - strand at 2175.053 kb on + strand at 2175.086 kb on + strand at 2175.096 kb on + strand at 2175.126 kb on + strand at 2175.196 kb on + strand at 2175.244 kb on + strand at 2175.244 kb on + strand at 2175.244 kb on + strand at 2175.288 kb on - strand at 2175.288 kb on - strand at 2175.288 kb on - strand at 2175.288 kb on - strand at 2175.288 kb on - strand at 2175.314 kb on + strand at 2175.361 kb on - strand at 2175.366 kb on - strand at 2175.397 kb on - strand at 2175.424 kb on - strand at 2175.424 kb on - strand at 2175.434 kb on - strand at 2175.441 kb on + strand at 2175.449 kb on - strand at 2175.484 kb on - strand at 2175.497 kb on + strand at 2175.497 kb on + strand at 2175.505 kb on - strand at 2175.507 kb on + strand at 2175.621 kb on - strand at 2175.678 kb on - strand, within Dsui_2056 at 2175.682 kb on + strand, within Dsui_2056 at 2175.682 kb on + strand, within Dsui_2056 at 2175.682 kb on + strand, within Dsui_2056 at 2175.690 kb on - strand, within Dsui_2056 at 2175.690 kb on - strand, within Dsui_2056 at 2175.690 kb on - strand, within Dsui_2056 at 2175.698 kb on + strand, within Dsui_2056 at 2175.706 kb on - strand, within Dsui_2056 at 2175.719 kb on + strand, within Dsui_2056 at 2175.719 kb on + strand, within Dsui_2056 at 2175.727 kb on - strand, within Dsui_2056 at 2175.727 kb on - strand, within Dsui_2056 at 2175.760 kb on - strand, within Dsui_2056 at 2175.760 kb on - strand, within Dsui_2056 at 2175.760 kb on - strand, within Dsui_2056 at 2175.801 kb on - strand at 2175.805 kb on + strand, within Dsui_2056 at 2175.811 kb on + strand, within Dsui_2056 at 2175.831 kb on - strand, within Dsui_2056 at 2175.867 kb on - strand, within Dsui_2056 at 2175.877 kb on - strand, within Dsui_2056 at 2175.887 kb on + strand, within Dsui_2056 at 2175.895 kb on + strand, within Dsui_2056 at 2175.912 kb on - strand, within Dsui_2056
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,173,147 + -2.2 2,173,153 + +1.0 2,173,160 + -0.6 2,173,168 - +1.0 2,173,181 + +1.0 2,173,243 + Dsui_2053 0.24 -2.3 2,173,359 - Dsui_2053 0.55 +1.1 2,173,448 + Dsui_2053 0.78 -0.6 2,173,503 - +1.0 2,173,503 - +1.0 2,173,525 + +1.0 2,173,592 + +1.0 2,173,624 + Dsui_2054 0.21 -0.0 2,173,714 - Dsui_2054 0.45 +0.6 2,173,778 + Dsui_2054 0.63 +1.0 2,173,809 - Dsui_2054 0.71 +1.0 2,173,941 - -0.6 2,174,022 - +1.0 2,174,058 + -0.0 2,174,089 - -0.0 2,174,099 - +1.0 2,174,182 + -0.6 2,174,189 + +1.0 2,174,262 - +1.0 2,174,271 - +1.0 2,174,401 - +1.0 2,174,654 - Dsui_2055 0.68 -0.0 2,174,775 - +1.0 2,174,823 - Dsui_2055 0.89 -0.0 2,174,922 - +1.0 2,174,927 - +1.0 2,174,953 - -1.3 2,175,053 + +1.0 2,175,086 + -1.0 2,175,096 + -1.0 2,175,126 + -0.0 2,175,196 + +1.0 2,175,244 + -0.0 2,175,244 + -0.0 2,175,244 + -0.0 2,175,288 - -0.0 2,175,288 - +1.0 2,175,288 - -0.0 2,175,288 - -0.0 2,175,288 - -0.6 2,175,314 + -1.0 2,175,361 - +1.0 2,175,366 - +1.0 2,175,397 - -0.0 2,175,424 - -1.0 2,175,424 - +1.0 2,175,434 - -0.0 2,175,441 + +1.0 2,175,449 - +1.0 2,175,484 - +1.0 2,175,497 + +1.0 2,175,497 + +1.0 2,175,505 - +1.0 2,175,507 + -0.0 2,175,621 - -0.0 2,175,678 - Dsui_2056 0.16 +1.0 2,175,682 + Dsui_2056 0.17 +1.0 2,175,682 + Dsui_2056 0.17 -1.8 2,175,682 + Dsui_2056 0.17 +1.0 2,175,690 - Dsui_2056 0.18 -0.6 2,175,690 - Dsui_2056 0.18 -1.3 2,175,690 - Dsui_2056 0.18 +1.0 2,175,698 + Dsui_2056 0.20 +1.0 2,175,706 - Dsui_2056 0.21 +1.0 2,175,719 + Dsui_2056 0.24 +1.0 2,175,719 + Dsui_2056 0.24 -0.6 2,175,727 - Dsui_2056 0.25 +1.0 2,175,727 - Dsui_2056 0.25 +1.0 2,175,760 - Dsui_2056 0.31 -1.3 2,175,760 - Dsui_2056 0.31 -0.6 2,175,760 - Dsui_2056 0.31 +1.0 2,175,801 - +1.0 2,175,805 + Dsui_2056 0.39 +1.0 2,175,811 + Dsui_2056 0.40 -0.6 2,175,831 - Dsui_2056 0.44 +1.0 2,175,867 - Dsui_2056 0.50 +1.0 2,175,877 - Dsui_2056 0.52 -1.6 2,175,887 + Dsui_2056 0.54 +1.0 2,175,895 + Dsui_2056 0.55 -2.8 2,175,912 - Dsui_2056 0.58 -1.3
Or see this region's nucleotide sequence