Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2049 and Dsui_2050 are separated by 59 nucleotides Dsui_2050 and Dsui_2051 are separated by 270 nucleotides Dsui_2051 and Dsui_2052 overlap by 4 nucleotides Dsui_2052 and Dsui_2053 are separated by 137 nucleotides Dsui_2053 and Dsui_2054 are separated by 16 nucleotides
Dsui_2049: Dsui_2049 - ABC-type uncharacterized transport system, permease and ATPase component, at 2,170,240 to 2,171,943
_2049
Dsui_2050: Dsui_2050 - hypothetical protein, at 2,172,003 to 2,172,377
_2050
Dsui_2051: Dsui_2051 - hypothetical protein, at 2,172,648 to 2,172,818
_2051
Dsui_2052: Dsui_2052 - hypothetical protein, at 2,172,815 to 2,173,012
_2052
Dsui_2053: Dsui_2053 - HNH endonuclease, at 2,173,150 to 2,173,530
_2053
Dsui_2054: Dsui_2054 - hypothetical protein, at 2,173,547 to 2,173,915
_2054
Position (kb)
2172
2173
2174 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2171.822 kb on + strand at 2171.850 kb on + strand at 2172.045 kb on - strand, within Dsui_2050 at 2172.106 kb on + strand, within Dsui_2050 at 2172.111 kb on + strand at 2172.114 kb on - strand, within Dsui_2050 at 2172.114 kb on - strand, within Dsui_2050 at 2172.119 kb on - strand, within Dsui_2050 at 2172.217 kb on - strand, within Dsui_2050 at 2172.386 kb on - strand at 2172.405 kb on - strand at 2172.667 kb on + strand, within Dsui_2051 at 2172.677 kb on + strand at 2172.729 kb on - strand, within Dsui_2051 at 2172.831 kb on + strand at 2172.908 kb on + strand, within Dsui_2052 at 2172.931 kb on - strand, within Dsui_2052 at 2172.964 kb on - strand, within Dsui_2052 at 2172.972 kb on + strand, within Dsui_2052 at 2173.083 kb on - strand at 2173.147 kb on + strand at 2173.153 kb on + strand at 2173.160 kb on + strand at 2173.168 kb on - strand at 2173.181 kb on + strand at 2173.243 kb on + strand, within Dsui_2053 at 2173.359 kb on - strand, within Dsui_2053 at 2173.448 kb on + strand, within Dsui_2053 at 2173.503 kb on - strand at 2173.503 kb on - strand at 2173.525 kb on + strand at 2173.592 kb on + strand at 2173.624 kb on + strand, within Dsui_2054 at 2173.714 kb on - strand, within Dsui_2054 at 2173.778 kb on + strand, within Dsui_2054 at 2173.809 kb on - strand, within Dsui_2054 at 2173.941 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,171,822 + +0.0 2,171,850 + +0.0 2,172,045 - Dsui_2050 0.11 +0.0 2,172,106 + Dsui_2050 0.27 -0.6 2,172,111 + +1.0 2,172,114 - Dsui_2050 0.30 +0.0 2,172,114 - Dsui_2050 0.30 -1.0 2,172,119 - Dsui_2050 0.31 -2.5 2,172,217 - Dsui_2050 0.57 +0.0 2,172,386 - +1.0 2,172,405 - -0.6 2,172,667 + Dsui_2051 0.11 -0.0 2,172,677 + +1.0 2,172,729 - Dsui_2051 0.47 +1.0 2,172,831 + -1.6 2,172,908 + Dsui_2052 0.47 -0.0 2,172,931 - Dsui_2052 0.59 -3.7 2,172,964 - Dsui_2052 0.75 +1.0 2,172,972 + Dsui_2052 0.79 -0.0 2,173,083 - -1.0 2,173,147 + -2.2 2,173,153 + +1.0 2,173,160 + -0.6 2,173,168 - +1.0 2,173,181 + +1.0 2,173,243 + Dsui_2053 0.24 -2.3 2,173,359 - Dsui_2053 0.55 +1.1 2,173,448 + Dsui_2053 0.78 -0.6 2,173,503 - +1.0 2,173,503 - +1.0 2,173,525 + +1.0 2,173,592 + +1.0 2,173,624 + Dsui_2054 0.21 -0.0 2,173,714 - Dsui_2054 0.45 +0.6 2,173,778 + Dsui_2054 0.63 +1.0 2,173,809 - Dsui_2054 0.71 +1.0 2,173,941 - -0.6
Or see this region's nucleotide sequence