Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2027 and Dsui_2028 are separated by 100 nucleotides Dsui_2028 and Dsui_2029 are separated by 182 nucleotides
Dsui_2027: Dsui_2027 - efflux transporter, outer membrane factor lipoprotein, NodT family, at 2,145,500 to 2,147,065
_2027
Dsui_2028: Dsui_2028 - putative endonuclease containing a URI domain, at 2,147,166 to 2,147,462
_2028
Dsui_2029: Dsui_2029 - putative hydrolase or acyltransferase of alpha/beta superfamily, at 2,147,645 to 2,148,565
_2029
Position (kb)
2147
2148 Strain fitness (log2 ratio)
-1
0
1 at 2146.199 kb on + strand at 2146.450 kb on + strand, within Dsui_2027 at 2146.541 kb on - strand at 2146.550 kb on + strand, within Dsui_2027 at 2146.625 kb on - strand at 2146.659 kb on + strand at 2146.750 kb on - strand at 2146.821 kb on + strand, within Dsui_2027 at 2146.879 kb on + strand at 2147.004 kb on - strand at 2147.027 kb on + strand at 2147.027 kb on + strand at 2147.323 kb on + strand at 2147.591 kb on - strand at 2147.603 kb on + strand at 2147.604 kb on - strand at 2147.616 kb on - strand at 2147.641 kb on + strand at 2147.658 kb on - strand at 2147.710 kb on - strand at 2147.967 kb on - strand, within Dsui_2029 at 2147.967 kb on - strand, within Dsui_2029 at 2148.089 kb on - strand, within Dsui_2029 at 2148.428 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,146,199 + +1.1 2,146,450 + Dsui_2027 0.61 -0.9 2,146,541 - -0.5 2,146,550 + Dsui_2027 0.67 -0.9 2,146,625 - -0.9 2,146,659 + +1.1 2,146,750 - +1.1 2,146,821 + Dsui_2027 0.84 -0.5 2,146,879 + +0.1 2,147,004 - +0.1 2,147,027 + +1.1 2,147,027 + -0.5 2,147,323 + +1.1 2,147,591 - +1.1 2,147,603 + -0.5 2,147,604 - -0.5 2,147,616 - +0.1 2,147,641 + -1.3 2,147,658 - -0.5 2,147,710 - -0.5 2,147,967 - Dsui_2029 0.35 -0.5 2,147,967 - Dsui_2029 0.35 +0.1 2,148,089 - Dsui_2029 0.48 -0.9 2,148,428 + +1.1
Or see this region's nucleotide sequence