Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1686 and Dsui_1687 are separated by 74 nucleotides Dsui_1687 and Dsui_1688 are separated by 192 nucleotides Dsui_1688 and Dsui_1689 are separated by 18 nucleotides
Dsui_1686: Dsui_1686 - NAD+ synthetase, at 1,773,136 to 1,774,761
_1686
Dsui_1687: Dsui_1687 - nitrogen regulatory protein PII, at 1,774,836 to 1,775,174
_1687
Dsui_1688: Dsui_1688 - hypothetical protein, at 1,775,367 to 1,776,413
_1688
Dsui_1689: Dsui_1689 - thioredoxin-disulfide reductase, at 1,776,432 to 1,777,391
_1689
Position (kb)
1775
1776
1777 Strain fitness (log2 ratio)
-2
-1
0
1 at 1774.740 kb on + strand at 1774.807 kb on - strand at 1774.851 kb on + strand at 1774.873 kb on - strand at 1774.883 kb on + strand, within Dsui_1687 at 1774.939 kb on - strand, within Dsui_1687 at 1775.014 kb on + strand, within Dsui_1687 at 1775.022 kb on - strand, within Dsui_1687 at 1775.074 kb on - strand, within Dsui_1687 at 1775.246 kb on - strand at 1775.272 kb on + strand at 1775.363 kb on + strand at 1775.541 kb on + strand at 1775.541 kb on + strand at 1775.627 kb on - strand, within Dsui_1688 at 1775.980 kb on + strand at 1776.077 kb on + strand, within Dsui_1688 at 1776.164 kb on - strand, within Dsui_1688 at 1776.212 kb on - strand, within Dsui_1688 at 1776.322 kb on + strand at 1776.322 kb on + strand at 1776.438 kb on - strand at 1776.440 kb on + strand at 1777.401 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 1,774,740 + -0.5 1,774,807 - +0.6 1,774,851 + -0.5 1,774,873 - +0.6 1,774,883 + Dsui_1687 0.14 -0.3 1,774,939 - Dsui_1687 0.30 +0.6 1,775,014 + Dsui_1687 0.53 -0.4 1,775,022 - Dsui_1687 0.55 +0.6 1,775,074 - Dsui_1687 0.70 +0.6 1,775,246 - -0.1 1,775,272 + -1.6 1,775,363 + +0.6 1,775,541 + -0.4 1,775,541 + +0.6 1,775,627 - Dsui_1688 0.25 +0.6 1,775,980 + +0.6 1,776,077 + Dsui_1688 0.68 -2.0 1,776,164 - Dsui_1688 0.76 +1.0 1,776,212 - Dsui_1688 0.81 +0.5 1,776,322 + -0.4 1,776,322 + +0.6 1,776,438 - +0.9 1,776,440 + +0.6 1,777,401 - -0.2
Or see this region's nucleotide sequence