Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1544 and Dsui_1545 are separated by 249 nucleotides Dsui_1545 and Dsui_1546 are separated by 36 nucleotides Dsui_1546 and Dsui_1547 are separated by 36 nucleotides Dsui_1547 and Dsui_1548 are separated by 67 nucleotides
Dsui_1544: Dsui_1544 - Protein of unknown function (DUF3108), at 1,604,835 to 1,605,572
_1544
Dsui_1545: Dsui_1545 - phosphoribosylglycinamide formyltransferase, formyltetrahydrofolate-dependent, at 1,605,822 to 1,606,478
_1545
Dsui_1546: Dsui_1546 - hypothetical protein, at 1,606,515 to 1,607,267
_1546
Dsui_1547: Dsui_1547 - putative membrane-associated protein, at 1,607,304 to 1,607,966
_1547
Dsui_1548: Dsui_1548 - response regulator with CheY-like receiver domain and winged-helix DNA-binding domain, at 1,608,034 to 1,608,480
_1548
Position (kb)
1606
1607
1608 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1605.751 kb on - strand at 1605.820 kb on + strand at 1606.574 kb on + strand at 1606.580 kb on + strand at 1606.792 kb on - strand, within Dsui_1546 at 1606.830 kb on + strand at 1606.847 kb on - strand, within Dsui_1546 at 1606.848 kb on - strand, within Dsui_1546 at 1606.921 kb on + strand, within Dsui_1546 at 1606.951 kb on + strand at 1606.996 kb on - strand, within Dsui_1546 at 1607.050 kb on - strand, within Dsui_1546 at 1607.050 kb on - strand, within Dsui_1546 at 1607.184 kb on - strand, within Dsui_1546 at 1607.225 kb on + strand at 1607.233 kb on - strand at 1607.319 kb on - strand at 1607.986 kb on - strand at 1608.008 kb on - strand at 1608.008 kb on - strand at 1608.008 kb on - strand at 1608.037 kb on + strand at 1608.074 kb on + strand at 1608.074 kb on + strand at 1608.074 kb on + strand at 1608.082 kb on - strand, within Dsui_1548 at 1608.084 kb on + strand, within Dsui_1548 at 1608.174 kb on + strand at 1608.186 kb on + strand at 1608.255 kb on + strand at 1608.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 1,605,751 - +0.5 1,605,820 + -2.0 1,606,574 + -2.0 1,606,580 + +0.6 1,606,792 - Dsui_1546 0.37 -0.3 1,606,830 + +0.6 1,606,847 - Dsui_1546 0.44 +0.6 1,606,848 - Dsui_1546 0.44 -1.0 1,606,921 + Dsui_1546 0.54 -1.0 1,606,951 + -1.0 1,606,996 - Dsui_1546 0.64 +1.8 1,607,050 - Dsui_1546 0.71 +0.7 1,607,050 - Dsui_1546 0.71 +1.4 1,607,184 - Dsui_1546 0.89 +0.5 1,607,225 + -0.4 1,607,233 - -0.5 1,607,319 - +0.6 1,607,986 - -1.8 1,608,008 - +1.7 1,608,008 - -1.4 1,608,008 - -0.0 1,608,037 + -0.4 1,608,074 + -1.8 1,608,074 + +0.6 1,608,074 + -1.8 1,608,082 - Dsui_1548 0.11 +0.6 1,608,084 + Dsui_1548 0.11 -1.0 1,608,174 + +0.6 1,608,186 + -0.4 1,608,255 + +0.6 1,608,263 - +0.6
Or see this region's nucleotide sequence