Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1543 and Dsui_1544 are separated by 73 nucleotides Dsui_1544 and Dsui_1545 are separated by 249 nucleotides Dsui_1545 and Dsui_1546 are separated by 36 nucleotides
Dsui_1543: Dsui_1543 - tRNA/rRNA cytosine-C5-methylase, at 1,603,433 to 1,604,761
_1543
Dsui_1544: Dsui_1544 - Protein of unknown function (DUF3108), at 1,604,835 to 1,605,572
_1544
Dsui_1545: Dsui_1545 - phosphoribosylglycinamide formyltransferase, formyltetrahydrofolate-dependent, at 1,605,822 to 1,606,478
_1545
Dsui_1546: Dsui_1546 - hypothetical protein, at 1,606,515 to 1,607,267
_1546
Position (kb)
1604
1605
1606 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1603.892 kb on - strand, within Dsui_1543 at 1603.894 kb on + strand at 1603.894 kb on + strand at 1603.923 kb on - strand, within Dsui_1543 at 1604.095 kb on + strand at 1604.254 kb on + strand at 1604.280 kb on - strand, within Dsui_1543 at 1604.307 kb on + strand at 1604.378 kb on + strand, within Dsui_1543 at 1604.380 kb on - strand, within Dsui_1543 at 1604.568 kb on - strand, within Dsui_1543 at 1605.085 kb on + strand at 1605.113 kb on - strand, within Dsui_1544 at 1605.120 kb on - strand, within Dsui_1544 at 1605.303 kb on + strand at 1605.397 kb on + strand, within Dsui_1544 at 1605.751 kb on - strand at 1605.820 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 1,603,892 - Dsui_1543 0.35 -0.0 1,603,894 + +0.5 1,603,894 + +0.5 1,603,923 - Dsui_1543 0.37 -0.4 1,604,095 + -0.5 1,604,254 + +0.5 1,604,280 - Dsui_1543 0.64 -0.2 1,604,307 + +0.5 1,604,378 + Dsui_1543 0.71 -2.3 1,604,380 - Dsui_1543 0.71 -0.0 1,604,568 - Dsui_1543 0.85 +0.1 1,605,085 + +0.5 1,605,113 - Dsui_1544 0.38 -3.9 1,605,120 - Dsui_1544 0.39 -3.7 1,605,303 + -1.5 1,605,397 + Dsui_1544 0.76 -0.5 1,605,751 - +0.5 1,605,820 + -2.0
Or see this region's nucleotide sequence