Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1469 and Dsui_1470 are separated by 78 nucleotides Dsui_1470 and Dsui_1471 are separated by 56 nucleotides Dsui_1471 and Dsui_1472 are separated by 4 nucleotides Dsui_1472 and Dsui_1473 are separated by 118 nucleotides
Dsui_1469: Dsui_1469 - L-aspartate-alpha-decarboxylase, at 1,532,826 to 1,533,206
_1469
Dsui_1470: Dsui_1470 - pantoate--beta-alanine ligase, at 1,533,285 to 1,534,109
_1470
Dsui_1471: Dsui_1471 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,534,166 to 1,534,978
_1471
Dsui_1472: Dsui_1472 - deoxynucleoside kinase, at 1,534,983 to 1,535,657
_1472
Dsui_1473: Dsui_1473 - methyl-accepting chemotaxis protein, at 1,535,776 to 1,536,906
_1473
Position (kb) 1534
1535 Strain fitness (log2 ratio) -2
-1
0
1 at 1534.139 kb on - strand at 1534.175 kb on - strand at 1534.175 kb on - strand at 1535.159 kb on + strand, within Dsui_1472 at 1535.279 kb on + strand, within Dsui_1472 at 1535.303 kb on + strand, within Dsui_1472 at 1535.389 kb on - strand, within Dsui_1472 at 1535.466 kb on + strand, within Dsui_1472 at 1535.536 kb on - strand, within Dsui_1472 at 1535.781 kb on + strand at 1535.789 kb on - strand at 1535.828 kb on - strand at 1535.946 kb on + strand, within Dsui_1473
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 1,534,139 - +1.0 1,534,175 - +1.2 1,534,175 - +1.4 1,535,159 + Dsui_1472 0.26 -0.1 1,535,279 + Dsui_1472 0.44 -0.4 1,535,303 + Dsui_1472 0.47 -2.4 1,535,389 - Dsui_1472 0.60 +0.2 1,535,466 + Dsui_1472 0.72 +0.6 1,535,536 - Dsui_1472 0.82 -0.3 1,535,781 + -1.0 1,535,789 - +0.6 1,535,828 - +0.6 1,535,946 + Dsui_1473 0.15 -0.5
Or see this region's nucleotide sequence