Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1366 and Dsui_1367 are separated by 120 nucleotides Dsui_1367 and Dsui_1368 are separated by 8 nucleotides Dsui_1368 and Dsui_1369 are separated by 50 nucleotides
Dsui_1366: Dsui_1366 - glutaminyl-tRNA synthetase, at 1,419,730 to 1,421,511
_1366
Dsui_1367: Dsui_1367 - crcB protein, at 1,421,632 to 1,422,021
_1367
Dsui_1368: Dsui_1368 - hypothetical protein, at 1,422,030 to 1,422,866
_1368
Dsui_1369: Dsui_1369 - hypothetical protein, at 1,422,917 to 1,423,207
_1369
Position (kb)
1421
1422
1423 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1421.588 kb on - strand at 1421.609 kb on + strand at 1421.609 kb on + strand at 1421.609 kb on + strand at 1421.609 kb on + strand at 1421.609 kb on + strand at 1421.617 kb on - strand at 1421.617 kb on - strand at 1421.624 kb on + strand at 1421.624 kb on - strand at 1421.632 kb on - strand at 1421.709 kb on + strand, within Dsui_1367 at 1421.721 kb on + strand, within Dsui_1367 at 1421.724 kb on - strand, within Dsui_1367 at 1421.724 kb on - strand at 1421.780 kb on + strand, within Dsui_1367 at 1421.814 kb on + strand, within Dsui_1367 at 1421.815 kb on - strand at 1421.826 kb on + strand, within Dsui_1367 at 1421.947 kb on - strand, within Dsui_1367 at 1422.015 kb on + strand at 1422.092 kb on - strand at 1422.103 kb on - strand at 1422.110 kb on - strand at 1422.152 kb on + strand, within Dsui_1368 at 1422.171 kb on - strand at 1422.175 kb on - strand at 1422.191 kb on - strand, within Dsui_1368 at 1422.217 kb on - strand, within Dsui_1368 at 1422.225 kb on - strand, within Dsui_1368 at 1422.239 kb on + strand, within Dsui_1368 at 1422.247 kb on - strand, within Dsui_1368 at 1422.350 kb on - strand at 1422.453 kb on + strand, within Dsui_1368 at 1422.466 kb on - strand, within Dsui_1368 at 1422.569 kb on + strand at 1422.569 kb on + strand, within Dsui_1368 at 1422.667 kb on - strand at 1422.713 kb on - strand at 1422.715 kb on + strand at 1422.743 kb on + strand, within Dsui_1368 at 1422.743 kb on + strand, within Dsui_1368 at 1422.751 kb on - strand at 1422.804 kb on + strand at 1422.812 kb on - strand at 1422.827 kb on + strand at 1422.904 kb on - strand at 1422.929 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 1,421,588 - -0.4 1,421,609 + -0.7 1,421,609 + -1.8 1,421,609 + +1.1 1,421,609 + +0.6 1,421,609 + -0.5 1,421,617 - -0.4 1,421,617 - +0.6 1,421,624 + +0.6 1,421,624 - +0.6 1,421,632 - -0.4 1,421,709 + Dsui_1367 0.20 -0.2 1,421,721 + Dsui_1367 0.23 -0.3 1,421,724 - Dsui_1367 0.24 +1.1 1,421,724 - +0.6 1,421,780 + Dsui_1367 0.38 -0.2 1,421,814 + Dsui_1367 0.47 -0.4 1,421,815 - +0.6 1,421,826 + Dsui_1367 0.50 -0.6 1,421,947 - Dsui_1367 0.81 -1.8 1,422,015 + +0.6 1,422,092 - +0.6 1,422,103 - +0.6 1,422,110 - -0.4 1,422,152 + Dsui_1368 0.15 -3.4 1,422,171 - -0.4 1,422,175 - -1.0 1,422,191 - Dsui_1368 0.19 -0.4 1,422,217 - Dsui_1368 0.22 -1.0 1,422,225 - Dsui_1368 0.23 -1.0 1,422,239 + Dsui_1368 0.25 -2.4 1,422,247 - Dsui_1368 0.26 -2.0 1,422,350 - +0.6 1,422,453 + Dsui_1368 0.51 -1.0 1,422,466 - Dsui_1368 0.52 -1.0 1,422,569 + -0.4 1,422,569 + Dsui_1368 0.64 -1.0 1,422,667 - +0.6 1,422,713 - +0.6 1,422,715 + +0.6 1,422,743 + Dsui_1368 0.85 +0.6 1,422,743 + Dsui_1368 0.85 +0.6 1,422,751 - +0.6 1,422,804 + -1.4 1,422,812 - -0.4 1,422,827 + +0.6 1,422,904 - -0.4 1,422,929 + +0.6
Or see this region's nucleotide sequence