Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1302 and Dsui_1303 are separated by 3 nucleotides Dsui_1303 and Dsui_1304 are separated by 178 nucleotides Dsui_1304 and Dsui_1305 are separated by 226 nucleotides
Dsui_1302: Dsui_1302 - RNA polymerase sigma factor, sigma-70 family, at 1,364,980 to 1,365,480
_1302
Dsui_1303: Dsui_1303 - hypothetical protein, at 1,365,484 to 1,365,864
_1303
Dsui_1304: Dsui_1304 - cytochrome c553, at 1,366,043 to 1,366,684
_1304
Dsui_1305: Dsui_1305 - ribosome biogenesis GTP-binding protein YsxC/EngB, at 1,366,911 to 1,367,669
_1305
Position (kb)
1366
1367 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1365.099 kb on - strand, within Dsui_1302 at 1365.099 kb on - strand, within Dsui_1302 at 1365.195 kb on + strand, within Dsui_1302 at 1365.203 kb on - strand, within Dsui_1302 at 1365.203 kb on - strand, within Dsui_1302 at 1365.203 kb on - strand at 1365.372 kb on + strand, within Dsui_1302 at 1365.380 kb on - strand, within Dsui_1302 at 1365.380 kb on - strand, within Dsui_1302 at 1365.423 kb on + strand, within Dsui_1302 at 1365.569 kb on + strand, within Dsui_1303 at 1365.570 kb on - strand, within Dsui_1303 at 1365.630 kb on - strand, within Dsui_1303 at 1365.740 kb on - strand, within Dsui_1303 at 1365.771 kb on + strand, within Dsui_1303 at 1365.781 kb on + strand, within Dsui_1303 at 1365.842 kb on - strand at 1365.868 kb on + strand at 1365.873 kb on + strand at 1365.878 kb on + strand at 1365.878 kb on + strand at 1365.886 kb on - strand at 1365.949 kb on + strand at 1365.952 kb on + strand at 1365.954 kb on + strand at 1365.957 kb on - strand at 1366.049 kb on - strand at 1366.518 kb on + strand, within Dsui_1304 at 1366.521 kb on - strand, within Dsui_1304 at 1366.592 kb on - strand, within Dsui_1304 at 1366.625 kb on - strand at 1366.628 kb on + strand at 1366.660 kb on - strand at 1366.661 kb on + strand at 1366.669 kb on - strand at 1366.669 kb on - strand at 1366.685 kb on + strand at 1366.694 kb on - strand at 1366.756 kb on + strand at 1366.764 kb on - strand at 1366.771 kb on + strand at 1366.800 kb on + strand at 1366.808 kb on - strand at 1366.812 kb on + strand at 1366.819 kb on + strand at 1366.820 kb on - strand at 1366.820 kb on - strand at 1366.820 kb on - strand at 1366.820 kb on - strand at 1366.825 kb on - strand at 1366.858 kb on + strand at 1366.873 kb on + strand at 1366.909 kb on + strand at 1367.600 kb on + strand at 1367.653 kb on - strand at 1367.653 kb on - strand at 1367.679 kb on + strand at 1367.680 kb on + strand at 1367.681 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 1,365,099 - Dsui_1302 0.24 -3.1 1,365,099 - Dsui_1302 0.24 -2.0 1,365,195 + Dsui_1302 0.43 -1.8 1,365,203 - Dsui_1302 0.45 +0.6 1,365,203 - Dsui_1302 0.45 -0.4 1,365,203 - +0.6 1,365,372 + Dsui_1302 0.78 -1.4 1,365,380 - Dsui_1302 0.80 +0.6 1,365,380 - Dsui_1302 0.80 -1.4 1,365,423 + Dsui_1302 0.88 +0.6 1,365,569 + Dsui_1303 0.22 -1.8 1,365,570 - Dsui_1303 0.23 -1.0 1,365,630 - Dsui_1303 0.38 +0.6 1,365,740 - Dsui_1303 0.67 -0.3 1,365,771 + Dsui_1303 0.75 -1.4 1,365,781 + Dsui_1303 0.78 -0.4 1,365,842 - -0.9 1,365,868 + -2.9 1,365,873 + -0.4 1,365,878 + -1.4 1,365,878 + +0.6 1,365,886 - +0.1 1,365,949 + +0.6 1,365,952 + -3.0 1,365,954 + -2.2 1,365,957 - -0.1 1,366,049 - +0.0 1,366,518 + Dsui_1304 0.74 +0.6 1,366,521 - Dsui_1304 0.74 +1.3 1,366,592 - Dsui_1304 0.86 +1.5 1,366,625 - +1.3 1,366,628 + +1.1 1,366,660 - +1.7 1,366,661 + +0.6 1,366,669 - +1.6 1,366,669 - +1.8 1,366,685 + +0.6 1,366,694 - +0.1 1,366,756 + -1.6 1,366,764 - -0.7 1,366,771 + +0.6 1,366,800 + -2.2 1,366,808 - -0.3 1,366,812 + +0.6 1,366,819 + -1.0 1,366,820 - -0.2 1,366,820 - -0.2 1,366,820 - -0.1 1,366,820 - -0.4 1,366,825 - +0.2 1,366,858 + -0.7 1,366,873 + -0.1 1,366,909 + +2.8 1,367,600 + +2.4 1,367,653 - +3.4 1,367,653 - +3.3 1,367,679 + +0.6 1,367,680 + -0.4 1,367,681 + -2.0
Or see this region's nucleotide sequence