Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1257 and Dsui_1258 are separated by 38 nucleotides Dsui_1258 and Dsui_1259 are separated by 120 nucleotides Dsui_1259 and Dsui_1260 are separated by 71 nucleotides
Dsui_1257: Dsui_1257 - DNA-directed DNA polymerase III PolC, at 1,318,586 to 1,322,041
_1257
Dsui_1258: Dsui_1258 - transcriptional regulator, at 1,322,080 to 1,322,991
_1258
Dsui_1259: Dsui_1259 - Pirin-related protein, at 1,323,112 to 1,323,822
_1259
Dsui_1260: Dsui_1260 - multimeric flavodoxin WrbA, at 1,323,894 to 1,324,469
_1260
Position (kb)
1322
1323 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1322.188 kb on - strand, within Dsui_1258 at 1322.338 kb on + strand, within Dsui_1258 at 1322.387 kb on + strand, within Dsui_1258 at 1322.401 kb on - strand, within Dsui_1258 at 1322.413 kb on - strand, within Dsui_1258 at 1322.495 kb on - strand, within Dsui_1258 at 1322.495 kb on - strand, within Dsui_1258 at 1322.637 kb on - strand, within Dsui_1258 at 1322.663 kb on + strand at 1322.663 kb on + strand, within Dsui_1258 at 1322.671 kb on - strand, within Dsui_1258 at 1322.684 kb on - strand, within Dsui_1258 at 1322.702 kb on + strand, within Dsui_1258 at 1322.707 kb on - strand, within Dsui_1258 at 1322.717 kb on - strand, within Dsui_1258 at 1322.786 kb on + strand at 1322.847 kb on + strand at 1322.855 kb on - strand, within Dsui_1258 at 1322.865 kb on - strand, within Dsui_1258 at 1322.890 kb on - strand, within Dsui_1258 at 1323.051 kb on + strand at 1323.127 kb on - strand at 1323.251 kb on + strand at 1323.251 kb on + strand at 1323.420 kb on + strand at 1323.516 kb on + strand at 1323.655 kb on - strand at 1323.816 kb on + strand at 1323.823 kb on + strand at 1323.833 kb on - strand at 1323.875 kb on - strand at 1323.887 kb on - strand at 1323.937 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 1,322,188 - Dsui_1258 0.12 -0.1 1,322,338 + Dsui_1258 0.28 +0.6 1,322,387 + Dsui_1258 0.34 -1.0 1,322,401 - Dsui_1258 0.35 +0.1 1,322,413 - Dsui_1258 0.37 -0.3 1,322,495 - Dsui_1258 0.46 -0.2 1,322,495 - Dsui_1258 0.46 +1.3 1,322,637 - Dsui_1258 0.61 +0.5 1,322,663 + +0.6 1,322,663 + Dsui_1258 0.64 -2.4 1,322,671 - Dsui_1258 0.65 -0.2 1,322,684 - Dsui_1258 0.66 +0.2 1,322,702 + Dsui_1258 0.68 +0.6 1,322,707 - Dsui_1258 0.69 -0.0 1,322,717 - Dsui_1258 0.70 +0.4 1,322,786 + +0.6 1,322,847 + -0.4 1,322,855 - Dsui_1258 0.85 +0.2 1,322,865 - Dsui_1258 0.86 +0.1 1,322,890 - Dsui_1258 0.89 +0.3 1,323,051 + +2.1 1,323,127 - -0.4 1,323,251 + +0.6 1,323,251 + +0.6 1,323,420 + +0.6 1,323,516 + +0.6 1,323,655 - -0.4 1,323,816 + -0.4 1,323,823 + +0.6 1,323,833 - +0.6 1,323,875 - -0.4 1,323,887 - +0.6 1,323,937 + +0.6
Or see this region's nucleotide sequence