Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1031 and Dsui_1032 are separated by 167 nucleotides Dsui_1032 and Dsui_1033 are separated by 3 nucleotides Dsui_1033 and Dsui_1034 overlap by 4 nucleotides
Dsui_1031: Dsui_1031 - formamidopyrimidine-DNA glycosylase Fpg, at 1,063,698 to 1,064,525
_1031
Dsui_1032: Dsui_1032 - Flp pilus assembly protein TadD, at 1,064,693 to 1,066,432
_1032
Dsui_1033: Dsui_1033 - outer membrane lipoprotein LolB, at 1,066,436 to 1,067,011
_1033
Dsui_1034: Dsui_1034 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 1,067,008 to 1,067,859
_1034
Position (kb)
1064
1065
1066
1067 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1063.742 kb on + strand at 1063.750 kb on - strand at 1063.815 kb on - strand, within Dsui_1031 at 1063.887 kb on + strand, within Dsui_1031 at 1064.017 kb on + strand, within Dsui_1031 at 1064.071 kb on + strand at 1064.089 kb on - strand at 1064.158 kb on - strand, within Dsui_1031 at 1064.184 kb on - strand, within Dsui_1031 at 1064.200 kb on - strand, within Dsui_1031 at 1064.228 kb on + strand, within Dsui_1031 at 1064.371 kb on - strand, within Dsui_1031 at 1064.407 kb on + strand, within Dsui_1031 at 1064.509 kb on - strand at 1064.562 kb on + strand at 1064.569 kb on - strand at 1064.587 kb on - strand at 1064.643 kb on - strand at 1064.648 kb on - strand at 1064.678 kb on - strand at 1064.777 kb on + strand at 1064.825 kb on + strand at 1065.021 kb on + strand, within Dsui_1032 at 1065.162 kb on - strand at 1065.469 kb on + strand, within Dsui_1032 at 1065.762 kb on + strand, within Dsui_1032 at 1066.109 kb on + strand, within Dsui_1032 at 1066.209 kb on + strand, within Dsui_1032 at 1066.272 kb on + strand at 1066.342 kb on + strand at 1066.439 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 1,063,742 + +0.6 1,063,750 - +0.6 1,063,815 - Dsui_1031 0.14 +0.0 1,063,887 + Dsui_1031 0.23 +0.6 1,064,017 + Dsui_1031 0.39 +0.6 1,064,071 + +0.6 1,064,089 - +2.2 1,064,158 - Dsui_1031 0.56 -0.5 1,064,184 - Dsui_1031 0.59 +0.9 1,064,200 - Dsui_1031 0.61 +2.6 1,064,228 + Dsui_1031 0.64 -1.4 1,064,371 - Dsui_1031 0.81 -1.4 1,064,407 + Dsui_1031 0.86 -0.4 1,064,509 - +0.7 1,064,562 + +0.6 1,064,569 - -0.4 1,064,587 - -1.4 1,064,643 - -1.0 1,064,648 - +0.6 1,064,678 - -0.4 1,064,777 + -2.5 1,064,825 + -4.8 1,065,021 + Dsui_1032 0.19 -5.0 1,065,162 - +0.6 1,065,469 + Dsui_1032 0.45 -3.8 1,065,762 + Dsui_1032 0.61 -6.7 1,066,109 + Dsui_1032 0.81 -4.1 1,066,209 + Dsui_1032 0.87 -3.2 1,066,272 + -4.5 1,066,342 + -3.9 1,066,439 - +0.6
Or see this region's nucleotide sequence