Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1013 and Dsui_1014 are separated by 69 nucleotides Dsui_1014 and Dsui_1015 are separated by 2 nucleotides Dsui_1015 and Dsui_1016 are separated by 17 nucleotides
Dsui_1013: Dsui_1013 - thiamine biosynthesis protein ThiS, at 1,046,193 to 1,046,402
_1013
Dsui_1014: Dsui_1014 - putative enzyme of thiazole biosynthesis, at 1,046,472 to 1,047,257
_1014
Dsui_1015: Dsui_1015 - hypothetical protein, at 1,047,260 to 1,048,507
_1015
Dsui_1016: Dsui_1016 - tRNA (guanine-N(7)-)-methyltransferase, at 1,048,525 to 1,049,280
_1016
Position (kb)
1047
1048
1049 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1047.308 kb on - strand at 1047.343 kb on + strand at 1047.410 kb on + strand, within Dsui_1015 at 1047.459 kb on - strand, within Dsui_1015 at 1047.484 kb on + strand, within Dsui_1015 at 1047.624 kb on - strand, within Dsui_1015 at 1047.872 kb on - strand at 1047.892 kb on + strand, within Dsui_1015 at 1047.957 kb on + strand, within Dsui_1015 at 1048.102 kb on + strand, within Dsui_1015 at 1048.264 kb on + strand, within Dsui_1015 at 1048.338 kb on + strand, within Dsui_1015 at 1048.443 kb on - strand at 1048.471 kb on + strand at 1048.546 kb on + strand at 1048.571 kb on - strand at 1048.571 kb on - strand at 1048.580 kb on + strand at 1048.603 kb on + strand, within Dsui_1016 at 1048.847 kb on + strand, within Dsui_1016 at 1048.855 kb on - strand, within Dsui_1016 at 1048.890 kb on + strand, within Dsui_1016 at 1049.029 kb on - strand, within Dsui_1016 at 1049.224 kb on - strand at 1049.248 kb on + strand at 1049.251 kb on - strand at 1049.386 kb on + strand at 1049.386 kb on + strand at 1049.386 kb on + strand at 1049.394 kb on + strand at 1049.394 kb on - strand at 1049.434 kb on + strand at 1049.434 kb on - strand at 1049.472 kb on - strand at 1049.499 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 1,047,308 - +0.6 1,047,343 + -4.0 1,047,410 + Dsui_1015 0.12 -4.4 1,047,459 - Dsui_1015 0.16 -1.7 1,047,484 + Dsui_1015 0.18 -4.4 1,047,624 - Dsui_1015 0.29 -1.7 1,047,872 - +0.6 1,047,892 + Dsui_1015 0.51 -4.8 1,047,957 + Dsui_1015 0.56 -5.3 1,048,102 + Dsui_1015 0.67 -4.4 1,048,264 + Dsui_1015 0.80 -1.8 1,048,338 + Dsui_1015 0.86 -2.0 1,048,443 - +0.6 1,048,471 + -0.6 1,048,546 + +1.4 1,048,571 - +0.6 1,048,571 - +0.6 1,048,580 + +1.4 1,048,603 + Dsui_1016 0.10 +1.5 1,048,847 + Dsui_1016 0.43 +1.9 1,048,855 - Dsui_1016 0.44 -0.2 1,048,890 + Dsui_1016 0.48 +1.9 1,049,029 - Dsui_1016 0.67 -1.0 1,049,224 - -0.4 1,049,248 + +1.9 1,049,251 - +0.6 1,049,386 + -0.3 1,049,386 + +0.5 1,049,386 + -0.5 1,049,394 + +0.5 1,049,394 - -3.2 1,049,434 + +0.7 1,049,434 - +0.6 1,049,472 - -1.4 1,049,499 - -0.4
Or see this region's nucleotide sequence