Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0953 and Dsui_0954 are separated by 13 nucleotides Dsui_0954 and Dsui_0955 are separated by 381 nucleotides
Dsui_0953: Dsui_0953 - ADP-ribosyl-(dinitrogen reductase) hydrolase, at 978,577 to 979,500
_0953
Dsui_0954: Dsui_0954 - Dinitrogenase reductase ADP-ribosyltransferase (DRAT), at 979,514 to 980,365
_0954
Dsui_0955: Dsui_0955 - nitrogenase iron protein, at 980,747 to 981,622
_0955
Position (kb) 979
980
981 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 978.728 kb on - strand, within Dsui_0953 at 978.754 kb on - strand, within Dsui_0953 at 978.831 kb on + strand, within Dsui_0953 at 978.836 kb on - strand at 978.963 kb on + strand, within Dsui_0953 at 978.971 kb on - strand, within Dsui_0953 at 979.038 kb on - strand at 979.213 kb on + strand, within Dsui_0953 at 979.379 kb on - strand, within Dsui_0953 at 979.511 kb on + strand at 979.526 kb on + strand at 979.587 kb on - strand at 979.636 kb on + strand, within Dsui_0954 at 979.644 kb on - strand, within Dsui_0954 at 979.668 kb on - strand at 979.837 kb on + strand at 979.846 kb on + strand, within Dsui_0954 at 979.956 kb on + strand at 980.077 kb on - strand, within Dsui_0954 at 980.163 kb on + strand, within Dsui_0954 at 980.242 kb on - strand, within Dsui_0954 at 980.522 kb on + strand at 980.675 kb on - strand at 980.686 kb on - strand at 980.688 kb on + strand at 980.694 kb on + strand at 980.838 kb on - strand at 980.917 kb on - strand, within Dsui_0955 at 981.100 kb on - strand at 981.249 kb on - strand, within Dsui_0955 at 981.249 kb on - strand, within Dsui_0955 at 981.303 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 978,728 - Dsui_0953 0.16 -1.5 978,754 - Dsui_0953 0.19 -1.1 978,831 + Dsui_0953 0.27 +0.5 978,836 - +0.5 978,963 + Dsui_0953 0.42 -0.5 978,971 - Dsui_0953 0.43 -0.9 979,038 - +0.5 979,213 + Dsui_0953 0.69 -1.1 979,379 - Dsui_0953 0.87 -0.5 979,511 + +0.5 979,526 + +2.1 979,587 - -0.5 979,636 + Dsui_0954 0.14 -1.5 979,644 - Dsui_0954 0.15 +0.5 979,668 - -0.5 979,837 + +0.5 979,846 + Dsui_0954 0.39 -1.5 979,956 + +0.5 980,077 - Dsui_0954 0.66 -1.1 980,163 + Dsui_0954 0.76 -1.5 980,242 - Dsui_0954 0.85 +0.5 980,522 + -0.5 980,675 - -2.6 980,686 - -2.9 980,688 + -0.5 980,694 + -0.5 980,838 - +0.5 980,917 - Dsui_0955 0.19 -1.5 981,100 - +0.5 981,249 - Dsui_0955 0.57 -0.5 981,249 - Dsui_0955 0.57 -2.1 981,303 - +0.5
Or see this region's nucleotide sequence