Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0942 and Dsui_0943 are separated by 333 nucleotides Dsui_0943 and Dsui_0944 are separated by 44 nucleotides Dsui_0944 and Dsui_0945 are separated by 156 nucleotides Dsui_0945 and Dsui_0946 are separated by 10 nucleotides
Dsui_0942: Dsui_0942 - hypothetical protein, at 970,797 to 971,366
_0942
Dsui_0943: Dsui_0943 - nitrogenase cofactor biosynthesis protein NifB, at 971,700 to 973,223
_0943
Dsui_0944: Dsui_0944 - putative NADH:ubiquinone oxidoreductase, subunit RnfB, at 973,268 to 973,483
_0944
Dsui_0945: Dsui_0945 - Iron-sulfur cluster assembly accessory protein, at 973,640 to 974,056
_0945
Dsui_0946: Dsui_0946 - putative nitrogen fixation protein FixT, at 974,067 to 974,561
_0946
Position (kb) 971
972
973
974 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 970.853 kb on - strand at 970.954 kb on + strand, within Dsui_0942 at 970.962 kb on - strand, within Dsui_0942 at 970.977 kb on + strand, within Dsui_0942 at 971.009 kb on + strand, within Dsui_0942 at 971.038 kb on + strand at 971.104 kb on - strand, within Dsui_0942 at 971.214 kb on - strand, within Dsui_0942 at 971.284 kb on + strand, within Dsui_0942 at 971.635 kb on - strand at 972.132 kb on + strand, within Dsui_0943 at 972.137 kb on + strand, within Dsui_0943 at 972.235 kb on + strand, within Dsui_0943 at 972.239 kb on + strand, within Dsui_0943 at 972.317 kb on - strand, within Dsui_0943 at 972.442 kb on + strand, within Dsui_0943 at 972.549 kb on - strand, within Dsui_0943 at 972.573 kb on - strand, within Dsui_0943 at 972.863 kb on - strand, within Dsui_0943 at 972.925 kb on + strand, within Dsui_0943 at 972.933 kb on - strand, within Dsui_0943 at 973.044 kb on + strand, within Dsui_0943 at 973.044 kb on + strand, within Dsui_0943 at 973.052 kb on - strand, within Dsui_0943 at 973.052 kb on - strand, within Dsui_0943 at 973.125 kb on + strand at 973.148 kb on + strand at 973.148 kb on + strand at 973.171 kb on + strand at 973.179 kb on - strand at 973.227 kb on - strand at 973.255 kb on - strand at 973.373 kb on + strand, within Dsui_0944 at 973.417 kb on + strand, within Dsui_0944 at 973.536 kb on + strand at 973.556 kb on - strand at 973.580 kb on - strand at 973.588 kb on + strand at 973.588 kb on + strand at 973.596 kb on + strand at 973.816 kb on + strand, within Dsui_0945 at 973.864 kb on + strand, within Dsui_0945 at 973.907 kb on - strand, within Dsui_0945 at 974.081 kb on - strand at 974.207 kb on - strand, within Dsui_0946
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 970,853 - +0.5 970,954 + Dsui_0942 0.28 -0.5 970,962 - Dsui_0942 0.29 +0.5 970,977 + Dsui_0942 0.32 +0.5 971,009 + Dsui_0942 0.37 -1.8 971,038 + +0.5 971,104 - Dsui_0942 0.54 +0.5 971,214 - Dsui_0942 0.73 +0.5 971,284 + Dsui_0942 0.85 -0.5 971,635 - -1.5 972,132 + Dsui_0943 0.28 -1.1 972,137 + Dsui_0943 0.29 +0.5 972,235 + Dsui_0943 0.35 -2.1 972,239 + Dsui_0943 0.35 -0.5 972,317 - Dsui_0943 0.40 +0.5 972,442 + Dsui_0943 0.49 +0.5 972,549 - Dsui_0943 0.56 +0.5 972,573 - Dsui_0943 0.57 -1.1 972,863 - Dsui_0943 0.76 -1.5 972,925 + Dsui_0943 0.80 -2.5 972,933 - Dsui_0943 0.81 -2.3 973,044 + Dsui_0943 0.88 -1.8 973,044 + Dsui_0943 0.88 +0.5 973,052 - Dsui_0943 0.89 -1.1 973,052 - Dsui_0943 0.89 +0.5 973,125 + +0.5 973,148 + +0.5 973,148 + -1.1 973,171 + +0.5 973,179 - -1.1 973,227 - -1.1 973,255 - -1.1 973,373 + Dsui_0944 0.49 -0.5 973,417 + Dsui_0944 0.69 -0.5 973,536 + -1.1 973,556 - -1.1 973,580 - -1.5 973,588 + -0.5 973,588 + +0.5 973,596 + -0.5 973,816 + Dsui_0945 0.42 +0.5 973,864 + Dsui_0945 0.54 -3.2 973,907 - Dsui_0945 0.64 -1.1 974,081 - -2.1 974,207 - Dsui_0946 0.28 -0.5
Or see this region's nucleotide sequence