Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0741 and Dsui_0742 are separated by 189 nucleotides Dsui_0742 and Dsui_0743 are separated by 88 nucleotides Dsui_0743 and Dsui_0744 are separated by 29 nucleotides Dsui_0744 and Dsui_0745 are separated by 199 nucleotides
Dsui_0741: Dsui_0741 - homoserine O-acetyltransferase, at 738,735 to 739,862
_0741
Dsui_0742: Dsui_0742 - phosphoenolpyruvate-protein phosphotransferase, at 740,052 to 741,791
_0742
Dsui_0743: Dsui_0743 - phosphotransferase system HPr (HPr) family protein, at 741,880 to 742,158
_0743
Dsui_0744: Dsui_0744 - phosphotransferase system, mannose/fructose-specific component IIA, at 742,188 to 742,580
_0744
Dsui_0745: Dsui_0745 - membrane-associated lipoprotein involved in thiamine biosynthesis, at 742,780 to 743,820
_0745
Position (kb) 740
741
742 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 739.938 kb on + strand at 739.940 kb on - strand at 739.967 kb on + strand at 739.998 kb on + strand at 740.031 kb on - strand at 740.098 kb on + strand at 740.101 kb on - strand at 740.112 kb on + strand at 740.128 kb on - strand at 740.266 kb on + strand, within Dsui_0742 at 740.274 kb on - strand, within Dsui_0742 at 740.302 kb on + strand, within Dsui_0742 at 740.381 kb on - strand, within Dsui_0742 at 740.532 kb on + strand, within Dsui_0742 at 740.549 kb on - strand, within Dsui_0742 at 740.559 kb on + strand, within Dsui_0742 at 740.601 kb on + strand, within Dsui_0742 at 740.646 kb on - strand, within Dsui_0742 at 740.646 kb on - strand, within Dsui_0742 at 740.702 kb on + strand, within Dsui_0742 at 740.721 kb on - strand, within Dsui_0742 at 740.731 kb on - strand, within Dsui_0742 at 740.756 kb on + strand, within Dsui_0742 at 740.764 kb on - strand, within Dsui_0742 at 740.949 kb on + strand, within Dsui_0742 at 740.957 kb on + strand, within Dsui_0742 at 740.962 kb on + strand, within Dsui_0742 at 740.989 kb on + strand, within Dsui_0742 at 740.989 kb on + strand, within Dsui_0742 at 741.139 kb on + strand, within Dsui_0742 at 741.167 kb on + strand at 741.170 kb on + strand, within Dsui_0742 at 741.188 kb on - strand, within Dsui_0742 at 741.193 kb on - strand, within Dsui_0742 at 741.277 kb on - strand, within Dsui_0742 at 741.286 kb on + strand, within Dsui_0742 at 741.407 kb on + strand, within Dsui_0742 at 741.421 kb on - strand, within Dsui_0742 at 741.438 kb on - strand, within Dsui_0742 at 741.542 kb on + strand, within Dsui_0742 at 741.586 kb on + strand, within Dsui_0742 at 741.615 kb on - strand, within Dsui_0742 at 741.617 kb on + strand, within Dsui_0742 at 741.693 kb on + strand at 741.707 kb on + strand at 741.715 kb on - strand at 741.738 kb on - strand at 741.798 kb on + strand at 741.806 kb on + strand at 741.921 kb on - strand, within Dsui_0743 at 741.921 kb on - strand, within Dsui_0743 at 741.957 kb on + strand, within Dsui_0743 at 741.965 kb on - strand, within Dsui_0743 at 742.049 kb on - strand, within Dsui_0743 at 742.147 kb on + strand at 742.152 kb on + strand at 742.193 kb on - strand at 742.194 kb on - strand at 742.199 kb on - strand at 742.236 kb on - strand, within Dsui_0744 at 742.295 kb on - strand, within Dsui_0744 at 742.324 kb on + strand, within Dsui_0744 at 742.511 kb on - strand, within Dsui_0744 at 742.530 kb on - strand, within Dsui_0744 at 742.532 kb on + strand, within Dsui_0744 at 742.584 kb on + strand at 742.644 kb on + strand at 742.668 kb on + strand at 742.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 739,938 + -1.6 739,940 - -0.4 739,967 + +0.4 739,998 + +0.4 740,031 - -0.1 740,098 + +0.4 740,101 - -4.9 740,112 + -2.9 740,128 - -4.4 740,266 + Dsui_0742 0.12 -1.1 740,274 - Dsui_0742 0.13 -6.3 740,302 + Dsui_0742 0.14 +0.4 740,381 - Dsui_0742 0.19 -5.7 740,532 + Dsui_0742 0.28 -0.6 740,549 - Dsui_0742 0.29 -5.3 740,559 + Dsui_0742 0.29 +0.4 740,601 + Dsui_0742 0.32 -1.6 740,646 - Dsui_0742 0.34 -7.4 740,646 - Dsui_0742 0.34 -4.9 740,702 + Dsui_0742 0.37 -0.6 740,721 - Dsui_0742 0.38 -4.9 740,731 - Dsui_0742 0.39 -5.7 740,756 + Dsui_0742 0.40 -0.6 740,764 - Dsui_0742 0.41 -4.9 740,949 + Dsui_0742 0.52 -1.1 740,957 + Dsui_0742 0.52 -3.3 740,962 + Dsui_0742 0.52 -1.1 740,989 + Dsui_0742 0.54 +0.4 740,989 + Dsui_0742 0.54 -1.6 741,139 + Dsui_0742 0.62 +0.4 741,167 + +0.4 741,170 + Dsui_0742 0.64 -0.6 741,188 - Dsui_0742 0.65 -5.8 741,193 - Dsui_0742 0.66 -5.7 741,277 - Dsui_0742 0.70 -3.6 741,286 + Dsui_0742 0.71 +0.4 741,407 + Dsui_0742 0.78 -1.1 741,421 - Dsui_0742 0.79 -5.6 741,438 - Dsui_0742 0.80 -5.7 741,542 + Dsui_0742 0.86 -0.6 741,586 + Dsui_0742 0.88 -1.2 741,615 - Dsui_0742 0.90 -4.9 741,617 + Dsui_0742 0.90 -1.9 741,693 + +0.4 741,707 + +0.4 741,715 - -6.1 741,738 - -5.6 741,798 + +0.4 741,806 + -2.4 741,921 - Dsui_0743 0.15 -5.9 741,921 - Dsui_0743 0.15 -5.4 741,957 + Dsui_0743 0.28 -1.6 741,965 - Dsui_0743 0.30 -4.9 742,049 - Dsui_0743 0.61 -5.3 742,147 + +0.4 742,152 + -0.6 742,193 - -0.3 742,194 - -0.7 742,199 - +0.8 742,236 - Dsui_0744 0.12 +0.6 742,295 - Dsui_0744 0.27 +1.1 742,324 + Dsui_0744 0.35 -2.8 742,511 - Dsui_0744 0.82 +1.2 742,530 - Dsui_0744 0.87 +1.2 742,532 + Dsui_0744 0.88 -1.2 742,584 + -0.6 742,644 + +4.1 742,668 + +0.4 742,771 - +0.0
Or see this region's nucleotide sequence