Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0511 and Dsui_0512 are separated by 318 nucleotides Dsui_0512 and Dsui_0513 are separated by 80 nucleotides Dsui_0513 and Dsui_0514 are separated by 96 nucleotides
Dsui_0511: Dsui_0511 - nitrate reductase cytochrome c-type subunit, at 515,592 to 516,068
_0511
Dsui_0512: Dsui_0512 - Methylmalonyl-CoA epimerase (EC 5.1.99.1) (from data) , at 516,387 to 516,863
_0512
Dsui_0513: Dsui_0513 - diguanylate cyclase (GGDEF) domain-containing protein, at 516,944 to 518,818
_0513
Dsui_0514: Dsui_0514 - hypothetical protein, at 518,915 to 520,018
_0514
Position (kb)
516
517
518
519 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 515.957 kb on + strand, within Dsui_0511 at 516.055 kb on - strand at 516.062 kb on + strand at 516.070 kb on - strand at 516.391 kb on - strand at 516.391 kb on - strand at 516.415 kb on + strand at 516.425 kb on - strand at 516.455 kb on + strand, within Dsui_0512 at 516.463 kb on - strand, within Dsui_0512 at 516.463 kb on - strand, within Dsui_0512 at 516.533 kb on + strand, within Dsui_0512 at 516.555 kb on + strand, within Dsui_0512 at 516.622 kb on + strand, within Dsui_0512 at 516.627 kb on + strand, within Dsui_0512 at 516.627 kb on + strand, within Dsui_0512 at 516.627 kb on + strand, within Dsui_0512 at 516.635 kb on - strand, within Dsui_0512 at 516.662 kb on + strand, within Dsui_0512 at 516.662 kb on + strand, within Dsui_0512 at 516.670 kb on - strand, within Dsui_0512 at 516.670 kb on - strand, within Dsui_0512 at 516.670 kb on - strand, within Dsui_0512 at 516.694 kb on - strand, within Dsui_0512 at 516.727 kb on - strand, within Dsui_0512 at 516.727 kb on - strand, within Dsui_0512 at 516.761 kb on - strand, within Dsui_0512 at 516.790 kb on + strand, within Dsui_0512 at 516.852 kb on + strand at 516.880 kb on - strand at 516.930 kb on - strand at 516.955 kb on + strand at 517.029 kb on - strand at 517.261 kb on + strand, within Dsui_0513 at 517.286 kb on + strand at 517.306 kb on + strand at 517.331 kb on + strand at 517.345 kb on - strand, within Dsui_0513 at 517.366 kb on + strand at 517.407 kb on - strand, within Dsui_0513 at 517.407 kb on - strand, within Dsui_0513 at 517.492 kb on + strand, within Dsui_0513 at 517.492 kb on + strand, within Dsui_0513 at 517.493 kb on + strand, within Dsui_0513 at 517.584 kb on + strand, within Dsui_0513 at 517.622 kb on - strand, within Dsui_0513 at 517.633 kb on + strand, within Dsui_0513 at 517.633 kb on + strand, within Dsui_0513 at 517.641 kb on - strand, within Dsui_0513 at 517.646 kb on - strand, within Dsui_0513 at 517.696 kb on + strand at 517.704 kb on - strand, within Dsui_0513 at 517.724 kb on - strand, within Dsui_0513 at 517.753 kb on + strand, within Dsui_0513 at 517.776 kb on - strand, within Dsui_0513 at 517.985 kb on - strand, within Dsui_0513 at 518.107 kb on - strand, within Dsui_0513 at 518.142 kb on + strand, within Dsui_0513 at 518.151 kb on - strand, within Dsui_0513 at 518.208 kb on - strand at 518.275 kb on + strand, within Dsui_0513 at 518.295 kb on - strand, within Dsui_0513 at 518.404 kb on - strand, within Dsui_0513 at 518.425 kb on - strand, within Dsui_0513 at 518.434 kb on + strand at 518.492 kb on - strand, within Dsui_0513 at 518.508 kb on - strand, within Dsui_0513 at 518.691 kb on - strand at 518.774 kb on + strand at 518.799 kb on + strand at 518.862 kb on - strand at 518.868 kb on + strand at 518.883 kb on - strand at 518.893 kb on - strand at 518.912 kb on + strand at 519.085 kb on - strand, within Dsui_0514 at 519.087 kb on + strand, within Dsui_0514 at 519.095 kb on + strand, within Dsui_0514 at 519.270 kb on - strand, within Dsui_0514 at 519.295 kb on + strand, within Dsui_0514 at 519.472 kb on - strand at 519.513 kb on + strand, within Dsui_0514 at 519.513 kb on + strand, within Dsui_0514 at 519.525 kb on + strand, within Dsui_0514 at 519.539 kb on - strand, within Dsui_0514 at 519.569 kb on - strand, within Dsui_0514 at 519.582 kb on + strand, within Dsui_0514 at 519.642 kb on - strand, within Dsui_0514 at 519.747 kb on + strand, within Dsui_0514 at 519.747 kb on + strand, within Dsui_0514 at 519.815 kb on + strand, within Dsui_0514 at 519.815 kb on + strand, within Dsui_0514
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 515,957 + Dsui_0511 0.77 -1.2 516,055 - +0.4 516,062 + -4.0 516,070 - -1.2 516,391 - -2.2 516,391 - +0.4 516,415 + +0.4 516,425 - -0.6 516,455 + Dsui_0512 0.14 +1.4 516,463 - Dsui_0512 0.16 -1.2 516,463 - Dsui_0512 0.16 +0.4 516,533 + Dsui_0512 0.31 +0.4 516,555 + Dsui_0512 0.35 -1.0 516,622 + Dsui_0512 0.49 +0.4 516,627 + Dsui_0512 0.50 +0.4 516,627 + Dsui_0512 0.50 -0.1 516,627 + Dsui_0512 0.50 -0.5 516,635 - Dsui_0512 0.52 +0.4 516,662 + Dsui_0512 0.58 -1.0 516,662 + Dsui_0512 0.58 +0.2 516,670 - Dsui_0512 0.59 -1.2 516,670 - Dsui_0512 0.59 +0.4 516,670 - Dsui_0512 0.59 -1.2 516,694 - Dsui_0512 0.64 +0.4 516,727 - Dsui_0512 0.71 -2.4 516,727 - Dsui_0512 0.71 -2.2 516,761 - Dsui_0512 0.78 -0.6 516,790 + Dsui_0512 0.84 -1.4 516,852 + -1.7 516,880 - -1.2 516,930 - -3.1 516,955 + +0.4 517,029 - -1.2 517,261 + Dsui_0513 0.17 -1.6 517,286 + +0.4 517,306 + +0.4 517,331 + +1.4 517,345 - Dsui_0513 0.21 -0.6 517,366 + +0.4 517,407 - Dsui_0513 0.25 -2.2 517,407 - Dsui_0513 0.25 +0.4 517,492 + Dsui_0513 0.29 -2.2 517,492 + Dsui_0513 0.29 +0.4 517,493 + Dsui_0513 0.29 -1.6 517,584 + Dsui_0513 0.34 -0.6 517,622 - Dsui_0513 0.36 -0.2 517,633 + Dsui_0513 0.37 -1.9 517,633 + Dsui_0513 0.37 -2.2 517,641 - Dsui_0513 0.37 -1.8 517,646 - Dsui_0513 0.37 -0.6 517,696 + +0.4 517,704 - Dsui_0513 0.41 -1.6 517,724 - Dsui_0513 0.42 -0.9 517,753 + Dsui_0513 0.43 -0.6 517,776 - Dsui_0513 0.44 -3.2 517,985 - Dsui_0513 0.56 -0.6 518,107 - Dsui_0513 0.62 -1.2 518,142 + Dsui_0513 0.64 +0.4 518,151 - Dsui_0513 0.64 -2.8 518,208 - +0.4 518,275 + Dsui_0513 0.71 -2.2 518,295 - Dsui_0513 0.72 +0.4 518,404 - Dsui_0513 0.78 -2.4 518,425 - Dsui_0513 0.79 +0.4 518,434 + +0.4 518,492 - Dsui_0513 0.83 +0.4 518,508 - Dsui_0513 0.83 -3.6 518,691 - -1.9 518,774 + -0.6 518,799 + -0.6 518,862 - +0.4 518,868 + +0.4 518,883 - -1.6 518,893 - +0.4 518,912 + -0.4 519,085 - Dsui_0514 0.15 +0.4 519,087 + Dsui_0514 0.16 -0.4 519,095 + Dsui_0514 0.16 +0.4 519,270 - Dsui_0514 0.32 +0.4 519,295 + Dsui_0514 0.34 +0.3 519,472 - +0.4 519,513 + Dsui_0514 0.54 -0.3 519,513 + Dsui_0514 0.54 -0.1 519,525 + Dsui_0514 0.55 +0.6 519,539 - Dsui_0514 0.57 -1.6 519,569 - Dsui_0514 0.59 -1.2 519,582 + Dsui_0514 0.60 -0.3 519,642 - Dsui_0514 0.66 -1.6 519,747 + Dsui_0514 0.75 -0.3 519,747 + Dsui_0514 0.75 +0.0 519,815 + Dsui_0514 0.82 -0.3 519,815 + Dsui_0514 0.82 +0.5
Or see this region's nucleotide sequence