Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0501 and Dsui_0502 are separated by 34 nucleotides Dsui_0502 and Dsui_0503 are separated by 46 nucleotides Dsui_0503 and Dsui_0504 overlap by 4 nucleotides
Dsui_0501: Dsui_0501 - glycine/D-amino acid oxidase, deaminating, at 503,490 to 504,740
_0501
Dsui_0502: Dsui_0502 - enoyl-CoA hydratase/carnithine racemase, at 504,775 to 505,566
_0502
Dsui_0503: Dsui_0503 - Mg-dependent DNase, at 505,613 to 506,473
_0503
Dsui_0504: Dsui_0504 - putative membrane protein, at 506,470 to 507,375
_0504
Position (kb) 504
505
506 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 504.780 kb on - strand at 504.780 kb on - strand at 504.808 kb on + strand at 504.828 kb on - strand at 504.830 kb on + strand at 504.945 kb on + strand, within Dsui_0502 at 505.044 kb on + strand, within Dsui_0502 at 505.052 kb on - strand, within Dsui_0502 at 505.060 kb on - strand, within Dsui_0502 at 505.244 kb on + strand, within Dsui_0502 at 505.325 kb on - strand, within Dsui_0502 at 505.340 kb on - strand at 505.407 kb on - strand, within Dsui_0502 at 505.410 kb on + strand, within Dsui_0502 at 505.410 kb on + strand, within Dsui_0502 at 505.618 kb on + strand at 505.713 kb on - strand at 505.713 kb on - strand at 505.810 kb on - strand at 505.810 kb on - strand at 505.875 kb on + strand, within Dsui_0503 at 505.914 kb on + strand, within Dsui_0503 at 505.966 kb on + strand, within Dsui_0503 at 505.989 kb on - strand, within Dsui_0503 at 506.024 kb on - strand, within Dsui_0503 at 506.101 kb on - strand, within Dsui_0503 at 506.134 kb on + strand, within Dsui_0503 at 506.456 kb on - strand at 506.530 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 504,780 - +0.4 504,780 - +0.4 504,808 + +0.4 504,828 - -0.7 504,830 + -0.1 504,945 + Dsui_0502 0.21 -0.6 505,044 + Dsui_0502 0.34 -0.5 505,052 - Dsui_0502 0.35 -1.6 505,060 - Dsui_0502 0.36 -1.6 505,244 + Dsui_0502 0.59 -0.4 505,325 - Dsui_0502 0.69 -3.0 505,340 - -0.6 505,407 - Dsui_0502 0.80 -2.2 505,410 + Dsui_0502 0.80 -0.4 505,410 + Dsui_0502 0.80 +0.7 505,618 + -0.1 505,713 - +0.4 505,713 - +0.4 505,810 - -0.6 505,810 - -1.9 505,875 + Dsui_0503 0.30 +1.7 505,914 + Dsui_0503 0.35 -0.8 505,966 + Dsui_0503 0.41 -1.0 505,989 - Dsui_0503 0.44 -1.2 506,024 - Dsui_0503 0.48 -0.6 506,101 - Dsui_0503 0.57 -1.2 506,134 + Dsui_0503 0.61 +1.3 506,456 - -1.9 506,530 - +0.4
Or see this region's nucleotide sequence