Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0484 and Dsui_0485 are separated by 74 nucleotides Dsui_0485 and Dsui_0486 overlap by 4 nucleotides Dsui_0486 and Dsui_0487 are separated by 156 nucleotides Dsui_0487 and Dsui_0488 overlap by 4 nucleotides
Dsui_0484: Dsui_0484 - Holliday junction DNA helicase, RuvB subunit, at 487,587 to 488,669
_0484
Dsui_0485: Dsui_0485 - Holliday junction DNA helicase, RuvA subunit, at 488,744 to 489,328
_0485
Dsui_0486: Dsui_0486 - crossover junction endodeoxyribonuclease RuvC, at 489,325 to 489,930
_0486
Dsui_0487: Dsui_0487 - hemerythrin HHE cation binding domain-containing protein, at 490,087 to 490,620
_0487
Dsui_0488: Dsui_0488 - EamA-like transporter family, at 490,617 to 491,474
_0488
Position (kb) 489
490 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 489.354 kb on - strand at 489.946 kb on - strand at 490.008 kb on - strand at 490.055 kb on + strand at 490.059 kb on + strand at 490.060 kb on + strand at 490.068 kb on - strand at 490.140 kb on + strand at 490.141 kb on - strand, within Dsui_0487 at 490.214 kb on - strand, within Dsui_0487 at 490.306 kb on - strand, within Dsui_0487 at 490.374 kb on + strand, within Dsui_0487 at 490.611 kb on - strand at 490.715 kb on + strand, within Dsui_0488 at 490.723 kb on - strand, within Dsui_0488 at 490.728 kb on - strand, within Dsui_0488 at 490.738 kb on - strand, within Dsui_0488 at 490.791 kb on - strand, within Dsui_0488 at 490.849 kb on + strand, within Dsui_0488 at 490.899 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 489,354 - -1.8 489,946 - +0.4 490,008 - -1.2 490,055 + -1.6 490,059 + -2.2 490,060 + -2.9 490,068 - +0.4 490,140 + -1.6 490,141 - Dsui_0487 0.10 +0.4 490,214 - Dsui_0487 0.24 -2.2 490,306 - Dsui_0487 0.41 -1.9 490,374 + Dsui_0487 0.54 -1.6 490,611 - -0.3 490,715 + Dsui_0488 0.11 -2.2 490,723 - Dsui_0488 0.12 +0.5 490,728 - Dsui_0488 0.13 -0.2 490,738 - Dsui_0488 0.14 -0.2 490,791 - Dsui_0488 0.20 -1.1 490,849 + Dsui_0488 0.27 -1.2 490,899 + +0.4
Or see this region's nucleotide sequence