Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0475 and Dsui_0476 are separated by 45 nucleotides Dsui_0476 and Dsui_0477 are separated by 115 nucleotides Dsui_0477 and Dsui_0478 are separated by 2 nucleotides Dsui_0478 and Dsui_0479 are separated by 39 nucleotides
Dsui_0475: Dsui_0475 - PilZ domain-containing protein, at 481,299 to 481,670
_0475
Dsui_0476: Dsui_0476 - organic radical activating enzyme, at 481,716 to 482,363
_0476
Dsui_0477: Dsui_0477 - tol-pal system protein YbgF, at 482,479 to 483,255
_0477
Dsui_0478: Dsui_0478 - peptidoglycan-associated lipoprotein, at 483,258 to 483,773
_0478
Dsui_0479: Dsui_0479 - tol-pal system beta propeller repeat protein TolB, at 483,813 to 485,096
_0479
Position (kb) 482
483
484 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 481.485 kb on + strand, within Dsui_0475 at 481.611 kb on + strand, within Dsui_0475 at 481.700 kb on + strand at 481.822 kb on - strand, within Dsui_0476 at 482.045 kb on + strand, within Dsui_0476 at 482.121 kb on + strand, within Dsui_0476 at 482.135 kb on - strand, within Dsui_0476 at 482.179 kb on + strand, within Dsui_0476 at 482.284 kb on + strand at 482.302 kb on - strand at 482.306 kb on + strand at 482.575 kb on - strand, within Dsui_0477 at 482.722 kb on - strand, within Dsui_0477 at 482.804 kb on + strand, within Dsui_0477 at 482.853 kb on + strand, within Dsui_0477 at 483.264 kb on - strand at 483.798 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 481,485 + Dsui_0475 0.50 -1.0 481,611 + Dsui_0475 0.84 -2.2 481,700 + -1.6 481,822 - Dsui_0476 0.16 +0.5 482,045 + Dsui_0476 0.51 +0.4 482,121 + Dsui_0476 0.62 +0.4 482,135 - Dsui_0476 0.65 +0.3 482,179 + Dsui_0476 0.71 -0.6 482,284 + +0.4 482,302 - +0.2 482,306 + +0.4 482,575 - Dsui_0477 0.12 -2.4 482,722 - Dsui_0477 0.31 -2.3 482,804 + Dsui_0477 0.42 -0.6 482,853 + Dsui_0477 0.48 -1.6 483,264 - -0.9 483,798 - -3.8
Or see this region's nucleotide sequence