Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0441 and Dsui_0442 are separated by 159 nucleotides Dsui_0442 and Dsui_0443 are separated by 6 nucleotides Dsui_0443 and Dsui_0444 are separated by 176 nucleotides
Dsui_0441: Dsui_0441 - Mg2+ transporter MgtE, at 448,010 to 449,452
_0441
Dsui_0442: Dsui_0442 - monofunctional biosynthetic peptidoglycan transglycosylase, at 449,612 to 450,304
_0442
Dsui_0443: Dsui_0443 - shikimate 5-dehydrogenase, at 450,311 to 451,126
_0443
Dsui_0444: Dsui_0444 - hemerythrin-like metal-binding domain-containing protein, at 451,303 to 451,761
_0444
Position (kb) 449
450
451 Strain fitness (log2 ratio) -2
-1
0
1 at 448.973 kb on - strand, within Dsui_0441 at 449.514 kb on + strand at 449.522 kb on - strand at 449.565 kb on - strand at 449.565 kb on - strand at 449.737 kb on + strand, within Dsui_0442 at 450.036 kb on + strand, within Dsui_0442 at 450.037 kb on - strand, within Dsui_0442 at 450.037 kb on - strand, within Dsui_0442 at 450.101 kb on - strand, within Dsui_0442 at 450.217 kb on + strand, within Dsui_0442 at 450.220 kb on - strand, within Dsui_0442 at 450.220 kb on - strand, within Dsui_0442 at 450.288 kb on - strand at 451.165 kb on + strand at 451.174 kb on - strand at 451.199 kb on - strand at 451.209 kb on - strand at 451.266 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 448,973 - Dsui_0441 0.67 -1.9 449,514 + -0.6 449,522 - -1.0 449,565 - -0.1 449,565 - -0.6 449,737 + Dsui_0442 0.18 -1.6 450,036 + Dsui_0442 0.61 -0.6 450,037 - Dsui_0442 0.61 -1.1 450,037 - Dsui_0442 0.61 -0.3 450,101 - Dsui_0442 0.71 -0.5 450,217 + Dsui_0442 0.87 -1.6 450,220 - Dsui_0442 0.88 -0.5 450,220 - Dsui_0442 0.88 +0.5 450,288 - +0.6 451,165 + -1.6 451,174 - +0.7 451,199 - -1.6 451,209 - -0.9 451,266 + +1.2
Or see this region's nucleotide sequence