Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0387 and Dsui_0388 are separated by 88 nucleotides Dsui_0388 and Dsui_0389 are separated by 12 nucleotides Dsui_0389 and Dsui_0390 are separated by 6 nucleotides Dsui_0390 and Dsui_0391 overlap by 4 nucleotides
Dsui_0387: Dsui_0387 - 3-dehydroquinate dehydratase, type II, at 386,578 to 387,078
_0387
Dsui_0388: Dsui_0388 - acetyl-CoA carboxylase, biotin carboxyl carrier protein, at 387,167 to 387,616
_0388
Dsui_0389: Dsui_0389 - acetyl-CoA carboxylase, biotin carboxylase subunit, at 387,629 to 388,996
_0389
Dsui_0390: Dsui_0390 - ribosomal protein L11 methyltransferase, at 389,003 to 389,890
_0390
Dsui_0391: Dsui_0391 - Protein of unknown function (DUF3426), at 389,887 to 390,879
_0391
Position (kb) 387
388
389 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 387.086 kb on + strand at 387.086 kb on + strand at 387.086 kb on + strand at 387.134 kb on + strand at 389.051 kb on + strand at 389.128 kb on - strand, within Dsui_0390 at 389.176 kb on - strand, within Dsui_0390 at 389.205 kb on - strand, within Dsui_0390 at 389.301 kb on + strand, within Dsui_0390 at 389.309 kb on - strand, within Dsui_0390 at 389.323 kb on + strand, within Dsui_0390 at 389.350 kb on + strand, within Dsui_0390 at 389.455 kb on + strand, within Dsui_0390 at 389.468 kb on - strand at 389.611 kb on + strand, within Dsui_0390 at 389.619 kb on - strand, within Dsui_0390 at 389.706 kb on - strand, within Dsui_0390 at 389.739 kb on - strand, within Dsui_0390 at 389.741 kb on + strand, within Dsui_0390 at 389.781 kb on - strand, within Dsui_0390 at 389.904 kb on + strand at 389.912 kb on - strand at 389.927 kb on + strand at 389.947 kb on - strand at 389.947 kb on - strand at 389.957 kb on - strand at 389.957 kb on - strand at 389.957 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 387,086 + +2.8 387,086 + +3.4 387,086 + +3.7 387,134 + +2.3 389,051 + +0.1 389,128 - Dsui_0390 0.14 +0.5 389,176 - Dsui_0390 0.19 -1.8 389,205 - Dsui_0390 0.23 -0.5 389,301 + Dsui_0390 0.34 -0.5 389,309 - Dsui_0390 0.34 -1.1 389,323 + Dsui_0390 0.36 +0.0 389,350 + Dsui_0390 0.39 +0.2 389,455 + Dsui_0390 0.51 +0.2 389,468 - +0.5 389,611 + Dsui_0390 0.68 +0.0 389,619 - Dsui_0390 0.69 -1.1 389,706 - Dsui_0390 0.79 -1.5 389,739 - Dsui_0390 0.83 -1.8 389,741 + Dsui_0390 0.83 -0.4 389,781 - Dsui_0390 0.88 -1.1 389,904 + -1.2 389,912 - -0.5 389,927 + -2.4 389,947 - -1.3 389,947 - -2.1 389,957 - -3.4 389,957 - -1.1 389,957 - -1.1
Or see this region's nucleotide sequence