Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0382 and Dsui_0383 are separated by 123 nucleotides Dsui_0383 and Dsui_0384 are separated by 99 nucleotides Dsui_0384 and Dsui_0385 are separated by 52 nucleotides Dsui_0385 and Dsui_0386 overlap by 4 nucleotides
Dsui_0382: Dsui_0382 - response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain, at 382,578 to 383,228
_0382
Dsui_0383: Dsui_0383 - hypothetical protein, at 383,352 to 383,864
_0383
Dsui_0384: Dsui_0384 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 383,964 to 385,313
_0384
Dsui_0385: Dsui_0385 - hypothetical protein, at 385,366 to 385,977
_0385
Dsui_0386: Dsui_0386 - thiol-disulfide isomerase-like thioredoxin, at 385,974 to 386,522
_0386
Position (kb) 383
384
385
386 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 382.965 kb on + strand, within Dsui_0382 at 383.032 kb on - strand, within Dsui_0382 at 383.032 kb on - strand, within Dsui_0382 at 383.139 kb on - strand, within Dsui_0382 at 383.175 kb on + strand at 383.175 kb on + strand at 383.183 kb on - strand at 383.183 kb on - strand at 383.238 kb on - strand at 383.271 kb on - strand at 383.281 kb on - strand at 383.321 kb on - strand at 383.350 kb on + strand at 383.354 kb on - strand at 383.358 kb on - strand at 383.413 kb on - strand at 383.432 kb on - strand, within Dsui_0383 at 383.440 kb on + strand, within Dsui_0383 at 383.528 kb on + strand, within Dsui_0383 at 383.543 kb on + strand, within Dsui_0383 at 383.573 kb on - strand, within Dsui_0383 at 383.632 kb on + strand, within Dsui_0383 at 383.640 kb on - strand, within Dsui_0383 at 383.734 kb on - strand, within Dsui_0383 at 383.762 kb on + strand, within Dsui_0383 at 383.770 kb on - strand, within Dsui_0383 at 383.783 kb on + strand, within Dsui_0383 at 383.789 kb on + strand, within Dsui_0383 at 383.789 kb on + strand, within Dsui_0383 at 383.828 kb on + strand at 383.833 kb on + strand at 383.905 kb on - strand at 383.977 kb on + strand at 384.149 kb on - strand, within Dsui_0384 at 384.165 kb on - strand, within Dsui_0384 at 384.293 kb on - strand, within Dsui_0384 at 384.299 kb on + strand, within Dsui_0384 at 384.363 kb on + strand at 384.640 kb on + strand, within Dsui_0384 at 384.823 kb on + strand, within Dsui_0384 at 384.858 kb on - strand, within Dsui_0384 at 384.943 kb on + strand at 384.943 kb on + strand at 384.961 kb on - strand, within Dsui_0384 at 385.040 kb on - strand, within Dsui_0384 at 385.055 kb on - strand, within Dsui_0384 at 385.257 kb on - strand at 385.262 kb on - strand at 385.270 kb on - strand at 385.281 kb on - strand at 385.282 kb on + strand at 385.328 kb on + strand at 385.351 kb on - strand at 385.360 kb on - strand at 385.369 kb on + strand at 385.371 kb on - strand at 385.372 kb on - strand at 385.736 kb on + strand, within Dsui_0385 at 385.745 kb on + strand, within Dsui_0385 at 385.745 kb on + strand, within Dsui_0385 at 385.754 kb on - strand at 385.787 kb on + strand, within Dsui_0385 at 385.787 kb on + strand, within Dsui_0385 at 385.856 kb on + strand, within Dsui_0385 at 385.856 kb on + strand, within Dsui_0385 at 385.864 kb on + strand, within Dsui_0385 at 385.869 kb on + strand, within Dsui_0385 at 385.877 kb on - strand, within Dsui_0385 at 385.887 kb on - strand, within Dsui_0385 at 385.887 kb on - strand, within Dsui_0385 at 385.887 kb on - strand, within Dsui_0385 at 385.963 kb on - strand at 386.139 kb on + strand, within Dsui_0386 at 386.151 kb on + strand, within Dsui_0386 at 386.170 kb on + strand, within Dsui_0386 at 386.170 kb on + strand, within Dsui_0386 at 386.178 kb on - strand at 386.180 kb on + strand, within Dsui_0386 at 386.214 kb on - strand, within Dsui_0386 at 386.214 kb on - strand, within Dsui_0386 at 386.229 kb on - strand, within Dsui_0386 at 386.280 kb on - strand, within Dsui_0386 at 386.295 kb on + strand, within Dsui_0386 at 386.295 kb on + strand, within Dsui_0386 at 386.303 kb on - strand, within Dsui_0386
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 382,965 + Dsui_0382 0.59 +0.5 383,032 - Dsui_0382 0.70 -1.1 383,032 - Dsui_0382 0.70 -0.2 383,139 - Dsui_0382 0.86 +0.5 383,175 + +0.5 383,175 + -2.3 383,183 - -0.7 383,183 - +0.3 383,238 - +0.0 383,271 - +0.5 383,281 - -1.5 383,321 - +0.5 383,350 + +0.2 383,354 - +0.5 383,358 - -2.1 383,413 - +0.5 383,432 - Dsui_0383 0.16 -1.1 383,440 + Dsui_0383 0.17 +1.5 383,528 + Dsui_0383 0.34 +0.1 383,543 + Dsui_0383 0.37 -1.7 383,573 - Dsui_0383 0.43 -1.1 383,632 + Dsui_0383 0.55 -0.3 383,640 - Dsui_0383 0.56 -0.5 383,734 - Dsui_0383 0.74 +0.1 383,762 + Dsui_0383 0.80 +0.1 383,770 - Dsui_0383 0.81 -2.1 383,783 + Dsui_0383 0.84 -0.2 383,789 + Dsui_0383 0.85 +0.0 383,789 + Dsui_0383 0.85 +0.7 383,828 + -0.0 383,833 + -1.3 383,905 - +0.4 383,977 + +0.5 384,149 - Dsui_0384 0.14 +1.6 384,165 - Dsui_0384 0.15 +0.9 384,293 - Dsui_0384 0.24 +0.9 384,299 + Dsui_0384 0.25 -1.1 384,363 + +0.5 384,640 + Dsui_0384 0.50 -1.5 384,823 + Dsui_0384 0.64 -1.1 384,858 - Dsui_0384 0.66 +2.5 384,943 + +0.5 384,943 + +0.5 384,961 - Dsui_0384 0.74 +0.6 385,040 - Dsui_0384 0.80 +1.5 385,055 - Dsui_0384 0.81 +0.5 385,257 - -0.9 385,262 - +1.1 385,270 - -1.5 385,281 - +1.5 385,282 + -0.5 385,328 + -1.4 385,351 - +0.4 385,360 - +0.5 385,369 + +0.4 385,371 - +0.5 385,372 - +0.5 385,736 + Dsui_0385 0.60 -1.0 385,745 + Dsui_0385 0.62 +0.2 385,745 + Dsui_0385 0.62 -0.9 385,754 - +0.5 385,787 + Dsui_0385 0.69 -1.8 385,787 + Dsui_0385 0.69 -0.1 385,856 + Dsui_0385 0.80 +0.3 385,856 + Dsui_0385 0.80 -1.7 385,864 + Dsui_0385 0.81 -0.4 385,869 + Dsui_0385 0.82 -0.9 385,877 - Dsui_0385 0.83 -1.8 385,887 - Dsui_0385 0.85 -0.1 385,887 - Dsui_0385 0.85 -1.5 385,887 - Dsui_0385 0.85 -1.1 385,963 - -1.5 386,139 + Dsui_0386 0.30 +1.5 386,151 + Dsui_0386 0.32 -1.8 386,170 + Dsui_0386 0.36 -0.6 386,170 + Dsui_0386 0.36 -0.5 386,178 - +0.5 386,180 + Dsui_0386 0.38 -0.1 386,214 - Dsui_0386 0.44 +1.5 386,214 - Dsui_0386 0.44 -2.1 386,229 - Dsui_0386 0.46 -0.5 386,280 - Dsui_0386 0.56 -1.8 386,295 + Dsui_0386 0.58 -3.5 386,295 + Dsui_0386 0.58 -1.1 386,303 - Dsui_0386 0.60 +0.5
Or see this region's nucleotide sequence