Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0250 and Dsui_0251 are separated by 99 nucleotides Dsui_0251 and Dsui_0252 are separated by 20 nucleotides Dsui_0252 and Dsui_0253 overlap by 20 nucleotides Dsui_0253 and Dsui_0254 are separated by 181 nucleotides
Dsui_0250: Dsui_0250 - dihydroorotase, homodimeric type, at 248,872 to 249,906
_0250
Dsui_0251: Dsui_0251 - putative S-adenosylmethionine-dependent methyltransferase, YraL family, at 250,006 to 250,848
_0251
Dsui_0252: Dsui_0252 - TIGR00252 family protein, at 250,869 to 251,273
_0252
Dsui_0253: Dsui_0253 - HIRAN domain-containing protein, at 251,254 to 251,631
_0253
Dsui_0254: Dsui_0254 - tyrosyl-tRNA synthetase, at 251,813 to 253,087
_0254
Position (kb)
250
251 Strain fitness (log2 ratio)
-2
-1
0
1 at 249.935 kb on - strand at 249.972 kb on - strand at 249.989 kb on - strand at 250.124 kb on - strand, within Dsui_0251 at 250.167 kb on - strand, within Dsui_0251 at 250.244 kb on + strand at 250.267 kb on - strand, within Dsui_0251 at 250.356 kb on - strand, within Dsui_0251 at 250.376 kb on + strand at 250.384 kb on - strand, within Dsui_0251 at 250.450 kb on - strand, within Dsui_0251 at 250.533 kb on - strand, within Dsui_0251 at 250.754 kb on - strand, within Dsui_0251 at 250.812 kb on - strand at 250.923 kb on - strand, within Dsui_0252 at 250.932 kb on + strand, within Dsui_0252 at 250.992 kb on - strand, within Dsui_0252 at 251.012 kb on + strand at 251.235 kb on + strand at 251.235 kb on + strand at 251.345 kb on + strand, within Dsui_0253 at 251.420 kb on + strand, within Dsui_0253 at 251.475 kb on + strand, within Dsui_0253 at 251.530 kb on + strand, within Dsui_0253 at 251.548 kb on - strand, within Dsui_0253 at 251.613 kb on + strand at 251.632 kb on - strand at 251.765 kb on - strand at 251.775 kb on + strand at 251.775 kb on + strand at 251.783 kb on - strand at 251.783 kb on - strand at 251.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 249,935 - +0.1 249,972 - -0.1 249,989 - -0.5 250,124 - Dsui_0251 0.14 -0.2 250,167 - Dsui_0251 0.19 -1.8 250,244 + +0.8 250,267 - Dsui_0251 0.31 -0.8 250,356 - Dsui_0251 0.42 +0.8 250,376 + +0.8 250,384 - Dsui_0251 0.45 -0.8 250,450 - Dsui_0251 0.53 -0.8 250,533 - Dsui_0251 0.63 -1.2 250,754 - Dsui_0251 0.89 -1.8 250,812 - +0.8 250,923 - Dsui_0252 0.13 +0.8 250,932 + Dsui_0252 0.16 -0.2 250,992 - Dsui_0252 0.30 -0.2 251,012 + +0.8 251,235 + -1.2 251,235 + -1.5 251,345 + Dsui_0253 0.24 -1.2 251,420 + Dsui_0253 0.44 -1.2 251,475 + Dsui_0253 0.58 +0.8 251,530 + Dsui_0253 0.73 -0.2 251,548 - Dsui_0253 0.78 -2.2 251,613 + -1.2 251,632 - -0.2 251,765 - +0.1 251,775 + +0.8 251,775 + -0.2 251,783 - +0.5 251,783 - -0.0 251,789 - +0.2
Or see this region's nucleotide sequence