Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0124 and Dsui_0125 are separated by 4 nucleotides Dsui_0125 and Dsui_0126 are separated by 108 nucleotides Dsui_0126 and Dsui_0127 are separated by 160 nucleotides
Dsui_0124: Dsui_0124 - lactate dehydrogenase-like oxidoreductase, at 121,634 to 122,602
_0124
Dsui_0125: Dsui_0125 - NAD-dependent aldehyde dehydrogenase, at 122,607 to 123,344
_0125
Dsui_0126: Dsui_0126 - (4Fe-4S) cluster-containing protein, at 123,453 to 124,892
_0126
Dsui_0127: Dsui_0127 - transcriptional regulator, at 125,053 to 125,886
_0127
Position (kb)
123
124
125 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 122.519 kb on - strand at 122.526 kb on + strand at 122.534 kb on - strand at 122.605 kb on + strand at 122.655 kb on - strand at 122.675 kb on - strand at 122.872 kb on - strand, within Dsui_0125 at 122.896 kb on + strand at 123.218 kb on + strand at 123.241 kb on - strand, within Dsui_0125 at 123.272 kb on - strand at 123.282 kb on - strand at 123.287 kb on - strand at 123.315 kb on + strand at 123.317 kb on - strand at 123.331 kb on + strand at 123.360 kb on - strand at 123.395 kb on - strand at 123.427 kb on + strand at 123.446 kb on - strand at 123.451 kb on + strand at 123.459 kb on - strand at 123.493 kb on + strand at 123.655 kb on - strand, within Dsui_0126 at 123.682 kb on - strand, within Dsui_0126 at 123.697 kb on - strand, within Dsui_0126 at 123.770 kb on - strand, within Dsui_0126 at 123.827 kb on - strand, within Dsui_0126 at 123.828 kb on - strand, within Dsui_0126 at 123.839 kb on - strand, within Dsui_0126 at 123.869 kb on - strand, within Dsui_0126 at 123.899 kb on - strand, within Dsui_0126 at 124.007 kb on + strand, within Dsui_0126 at 124.007 kb on + strand at 124.035 kb on - strand, within Dsui_0126 at 124.035 kb on - strand, within Dsui_0126 at 124.092 kb on + strand, within Dsui_0126 at 124.111 kb on + strand at 124.127 kb on + strand, within Dsui_0126 at 124.220 kb on - strand, within Dsui_0126 at 124.276 kb on + strand, within Dsui_0126 at 124.286 kb on - strand at 124.322 kb on - strand, within Dsui_0126 at 124.332 kb on + strand at 124.348 kb on - strand, within Dsui_0126 at 124.396 kb on - strand, within Dsui_0126 at 124.396 kb on - strand, within Dsui_0126 at 124.533 kb on - strand, within Dsui_0126 at 124.543 kb on - strand, within Dsui_0126 at 124.567 kb on - strand, within Dsui_0126 at 124.740 kb on - strand, within Dsui_0126 at 124.759 kb on - strand at 124.781 kb on + strand at 124.785 kb on - strand at 124.828 kb on - strand at 124.848 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.910 kb on + strand at 125.014 kb on - strand at 125.072 kb on + strand at 125.080 kb on - strand at 125.135 kb on + strand at 125.204 kb on + strand, within Dsui_0127 at 125.204 kb on + strand, within Dsui_0127 at 125.204 kb on + strand, within Dsui_0127 at 125.212 kb on - strand, within Dsui_0127 at 125.217 kb on + strand, within Dsui_0127 at 125.217 kb on + strand, within Dsui_0127 at 125.240 kb on - strand, within Dsui_0127 at 125.266 kb on + strand, within Dsui_0127 at 125.309 kb on + strand, within Dsui_0127 at 125.383 kb on - strand, within Dsui_0127 at 125.388 kb on - strand, within Dsui_0127 at 125.399 kb on - strand, within Dsui_0127 at 125.411 kb on + strand, within Dsui_0127 at 125.429 kb on + strand, within Dsui_0127 at 125.482 kb on - strand, within Dsui_0127 at 125.514 kb on + strand, within Dsui_0127 at 125.536 kb on - strand, within Dsui_0127 at 125.541 kb on - strand, within Dsui_0127 at 125.556 kb on + strand, within Dsui_0127 at 125.556 kb on + strand, within Dsui_0127 at 125.564 kb on - strand, within Dsui_0127 at 125.816 kb on + strand at 125.837 kb on + strand at 125.857 kb on + strand at 125.857 kb on + strand at 125.858 kb on - strand at 125.862 kb on + strand at 125.875 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 122,519 - +0.8 122,526 + +0.9 122,534 - -0.1 122,605 + -0.1 122,655 - -0.2 122,675 - -0.1 122,872 - Dsui_0125 0.36 +0.3 122,896 + +0.9 123,218 + +0.9 123,241 - Dsui_0125 0.86 +0.9 123,272 - -0.2 123,282 - +1.3 123,287 - +1.8 123,315 + +0.9 123,317 - -0.2 123,331 + -2.1 123,360 - +0.1 123,395 - -0.7 123,427 + +0.9 123,446 - +0.9 123,451 + -1.9 123,459 - +0.1 123,493 + -0.7 123,655 - Dsui_0126 0.14 +0.1 123,682 - Dsui_0126 0.16 +0.4 123,697 - Dsui_0126 0.17 +1.0 123,770 - Dsui_0126 0.22 -0.0 123,827 - Dsui_0126 0.26 +0.2 123,828 - Dsui_0126 0.26 -0.2 123,839 - Dsui_0126 0.27 +0.3 123,869 - Dsui_0126 0.29 -0.3 123,899 - Dsui_0126 0.31 +0.1 124,007 + Dsui_0126 0.38 -0.7 124,007 + +0.9 124,035 - Dsui_0126 0.40 +1.5 124,035 - Dsui_0126 0.40 +0.0 124,092 + Dsui_0126 0.44 -2.5 124,111 + +0.9 124,127 + Dsui_0126 0.47 -0.1 124,220 - Dsui_0126 0.53 +0.8 124,276 + Dsui_0126 0.57 -1.1 124,286 - +1.1 124,322 - Dsui_0126 0.60 -0.2 124,332 + -0.1 124,348 - Dsui_0126 0.62 +0.3 124,396 - Dsui_0126 0.65 -0.6 124,396 - Dsui_0126 0.65 -0.4 124,533 - Dsui_0126 0.75 +0.1 124,543 - Dsui_0126 0.76 +0.2 124,567 - Dsui_0126 0.77 -0.5 124,740 - Dsui_0126 0.89 +0.5 124,759 - +0.8 124,781 + -1.1 124,785 - +0.4 124,828 - +0.2 124,848 + +0.9 124,900 + +0.9 124,900 + +0.9 124,900 + +0.5 124,900 + +0.9 124,900 + +0.9 124,900 + +0.9 124,900 + +0.9 124,908 - +0.3 124,908 - -0.9 124,908 - -3.4 124,908 - +0.1 124,908 - -0.1 124,908 - +0.3 124,908 - +1.0 124,908 - -0.1 124,908 - -1.3 124,910 + -0.1 125,014 - +0.9 125,072 + -0.8 125,080 - +0.9 125,135 + -0.4 125,204 + Dsui_0127 0.18 -0.7 125,204 + Dsui_0127 0.18 -1.0 125,204 + Dsui_0127 0.18 +0.6 125,212 - Dsui_0127 0.19 -0.7 125,217 + Dsui_0127 0.20 -0.7 125,217 + Dsui_0127 0.20 -1.3 125,240 - Dsui_0127 0.22 -0.1 125,266 + Dsui_0127 0.26 -1.0 125,309 + Dsui_0127 0.31 -0.5 125,383 - Dsui_0127 0.40 -2.1 125,388 - Dsui_0127 0.40 -1.9 125,399 - Dsui_0127 0.41 +0.9 125,411 + Dsui_0127 0.43 -0.3 125,429 + Dsui_0127 0.45 -2.1 125,482 - Dsui_0127 0.51 +0.9 125,514 + Dsui_0127 0.55 -0.6 125,536 - Dsui_0127 0.58 +0.9 125,541 - Dsui_0127 0.59 +0.9 125,556 + Dsui_0127 0.60 -0.7 125,556 + Dsui_0127 0.60 -0.6 125,564 - Dsui_0127 0.61 -2.1 125,816 + -0.7 125,837 + -0.9 125,857 + -0.4 125,857 + -1.7 125,858 - -0.5 125,862 + +0.3 125,875 - -2.3
Or see this region's nucleotide sequence