Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0085 and Dsui_0086 are separated by 14 nucleotides Dsui_0086 and Dsui_0087 overlap by 4 nucleotides Dsui_0087 and Dsui_0088 are separated by 178 nucleotides Dsui_0088 and Dsui_0089 are separated by 55 nucleotides
Dsui_0085: Dsui_0085 - GDP-mannose 4,6-dehydratase, at 83,344 to 84,435
_0085
Dsui_0086: Dsui_0086 - nucleoside-diphosphate-sugar epimerase, at 84,450 to 85,421
_0086
Dsui_0087: Dsui_0087 - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 85,418 to 86,854
_0087
Dsui_0088: Dsui_0088 - tRNA-Ala, at 87,033 to 87,108
_0088
Dsui_0089: Dsui_0089 - methyl-accepting chemotaxis protein, at 87,164 to 88,798
_0089
Position (kb)
85
86
87 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 84.425 kb on + strand at 84.439 kb on + strand at 84.439 kb on + strand at 84.447 kb on - strand at 84.447 kb on - strand at 84.483 kb on + strand at 84.483 kb on + strand at 84.491 kb on - strand at 84.491 kb on - strand at 84.491 kb on - strand at 84.501 kb on + strand at 84.545 kb on + strand at 84.545 kb on + strand at 84.581 kb on - strand, within Dsui_0086 at 84.690 kb on + strand, within Dsui_0086 at 84.765 kb on - strand, within Dsui_0086 at 84.773 kb on + strand, within Dsui_0086 at 84.804 kb on + strand, within Dsui_0086 at 84.804 kb on + strand, within Dsui_0086 at 84.843 kb on - strand, within Dsui_0086 at 84.858 kb on + strand, within Dsui_0086 at 84.858 kb on + strand, within Dsui_0086 at 84.907 kb on + strand, within Dsui_0086 at 84.959 kb on - strand, within Dsui_0086 at 84.977 kb on + strand, within Dsui_0086 at 84.985 kb on - strand, within Dsui_0086 at 84.985 kb on - strand, within Dsui_0086 at 85.022 kb on + strand, within Dsui_0086 at 85.022 kb on + strand, within Dsui_0086 at 85.030 kb on - strand, within Dsui_0086 at 85.030 kb on - strand, within Dsui_0086 at 85.030 kb on - strand, within Dsui_0086 at 85.030 kb on - strand, within Dsui_0086 at 85.030 kb on - strand, within Dsui_0086 at 85.030 kb on - strand, within Dsui_0086 at 85.040 kb on + strand at 85.048 kb on - strand, within Dsui_0086 at 85.073 kb on + strand, within Dsui_0086 at 85.073 kb on + strand, within Dsui_0086 at 85.164 kb on - strand, within Dsui_0086 at 85.187 kb on + strand, within Dsui_0086 at 85.218 kb on - strand, within Dsui_0086 at 85.231 kb on + strand, within Dsui_0086 at 85.326 kb on - strand at 85.422 kb on - strand at 85.442 kb on - strand at 85.488 kb on - strand at 85.538 kb on + strand at 85.566 kb on - strand, within Dsui_0087 at 85.566 kb on - strand, within Dsui_0087 at 85.631 kb on - strand, within Dsui_0087 at 85.734 kb on - strand, within Dsui_0087 at 85.743 kb on - strand, within Dsui_0087 at 85.788 kb on + strand, within Dsui_0087 at 85.796 kb on - strand, within Dsui_0087 at 85.826 kb on - strand, within Dsui_0087 at 85.826 kb on - strand, within Dsui_0087 at 85.833 kb on + strand, within Dsui_0087 at 85.833 kb on + strand, within Dsui_0087 at 85.836 kb on - strand, within Dsui_0087 at 85.841 kb on - strand, within Dsui_0087 at 85.861 kb on + strand, within Dsui_0087 at 85.903 kb on - strand, within Dsui_0087 at 86.184 kb on + strand, within Dsui_0087 at 86.218 kb on + strand, within Dsui_0087 at 86.284 kb on + strand, within Dsui_0087 at 86.292 kb on - strand, within Dsui_0087 at 86.394 kb on - strand, within Dsui_0087 at 86.433 kb on - strand, within Dsui_0087 at 86.455 kb on - strand, within Dsui_0087 at 86.544 kb on - strand, within Dsui_0087 at 86.554 kb on - strand, within Dsui_0087 at 86.564 kb on - strand, within Dsui_0087 at 86.584 kb on + strand, within Dsui_0087 at 86.698 kb on + strand at 86.698 kb on + strand, within Dsui_0087 at 86.801 kb on - strand at 86.825 kb on - strand at 86.833 kb on + strand at 86.879 kb on + strand at 86.919 kb on + strand at 86.929 kb on + strand at 86.929 kb on + strand at 86.937 kb on - strand at 86.973 kb on + strand at 87.016 kb on + strand at 87.017 kb on - strand at 87.021 kb on + strand at 87.040 kb on - strand at 87.040 kb on - strand at 87.056 kb on + strand, within Dsui_0088 at 87.064 kb on - strand, within Dsui_0088 at 87.087 kb on + strand, within Dsui_0088 at 87.094 kb on - strand, within Dsui_0088 at 87.136 kb on + strand at 87.136 kb on + strand at 87.136 kb on + strand at 87.136 kb on + strand at 87.136 kb on + strand at 87.136 kb on + strand at 87.144 kb on - strand at 87.144 kb on - strand at 87.180 kb on - strand at 87.237 kb on + strand at 87.250 kb on - strand at 87.278 kb on - strand at 87.284 kb on - strand at 87.323 kb on - strand at 87.330 kb on + strand, within Dsui_0089 at 87.340 kb on + strand, within Dsui_0089 at 87.343 kb on - strand, within Dsui_0089 at 87.402 kb on + strand, within Dsui_0089 at 87.490 kb on + strand, within Dsui_0089 at 87.549 kb on - strand, within Dsui_0089 at 87.564 kb on - strand, within Dsui_0089 at 87.587 kb on - strand, within Dsui_0089 at 87.768 kb on + strand, within Dsui_0089 at 87.783 kb on + strand, within Dsui_0089 at 87.818 kb on + strand, within Dsui_0089
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 84,425 + +0.9 84,439 + +0.9 84,439 + -0.7 84,447 - -2.9 84,447 - -3.3 84,483 + +0.9 84,483 + +0.9 84,491 - -2.3 84,491 - -3.2 84,491 - -3.7 84,501 + +0.9 84,545 + +0.9 84,545 + +0.9 84,581 - Dsui_0086 0.13 -4.1 84,690 + Dsui_0086 0.25 -0.7 84,765 - Dsui_0086 0.32 -3.1 84,773 + Dsui_0086 0.33 +0.9 84,804 + Dsui_0086 0.36 -0.1 84,804 + Dsui_0086 0.36 -0.1 84,843 - Dsui_0086 0.40 -3.7 84,858 + Dsui_0086 0.42 -0.1 84,858 + Dsui_0086 0.42 +0.9 84,907 + Dsui_0086 0.47 -0.1 84,959 - Dsui_0086 0.52 -1.7 84,977 + Dsui_0086 0.54 -0.1 84,985 - Dsui_0086 0.55 -3.3 84,985 - Dsui_0086 0.55 -3.4 85,022 + Dsui_0086 0.59 +0.9 85,022 + Dsui_0086 0.59 +0.9 85,030 - Dsui_0086 0.60 -3.4 85,030 - Dsui_0086 0.60 -3.9 85,030 - Dsui_0086 0.60 -3.7 85,030 - Dsui_0086 0.60 -1.9 85,030 - Dsui_0086 0.60 -3.7 85,030 - Dsui_0086 0.60 -2.8 85,040 + +0.9 85,048 - Dsui_0086 0.62 -2.3 85,073 + Dsui_0086 0.64 -0.1 85,073 + Dsui_0086 0.64 +0.9 85,164 - Dsui_0086 0.73 -3.0 85,187 + Dsui_0086 0.76 -1.1 85,218 - Dsui_0086 0.79 -2.7 85,231 + Dsui_0086 0.80 +0.9 85,326 - -3.3 85,422 - -0.2 85,442 - -0.7 85,488 - -3.5 85,538 + +0.9 85,566 - Dsui_0087 0.10 -2.4 85,566 - Dsui_0087 0.10 -2.9 85,631 - Dsui_0087 0.15 -3.4 85,734 - Dsui_0087 0.22 -3.3 85,743 - Dsui_0087 0.23 -2.6 85,788 + Dsui_0087 0.26 +0.9 85,796 - Dsui_0087 0.26 -3.9 85,826 - Dsui_0087 0.28 -3.4 85,826 - Dsui_0087 0.28 -2.3 85,833 + Dsui_0087 0.29 -1.7 85,833 + Dsui_0087 0.29 +0.9 85,836 - Dsui_0087 0.29 -2.5 85,841 - Dsui_0087 0.29 -3.2 85,861 + Dsui_0087 0.31 +0.9 85,903 - Dsui_0087 0.34 -2.8 86,184 + Dsui_0087 0.53 +0.9 86,218 + Dsui_0087 0.56 -0.1 86,284 + Dsui_0087 0.60 -0.1 86,292 - Dsui_0087 0.61 -3.3 86,394 - Dsui_0087 0.68 -2.1 86,433 - Dsui_0087 0.71 -3.4 86,455 - Dsui_0087 0.72 -3.0 86,544 - Dsui_0087 0.78 -3.0 86,554 - Dsui_0087 0.79 -2.0 86,564 - Dsui_0087 0.80 -2.4 86,584 + Dsui_0087 0.81 -0.1 86,698 + +0.9 86,698 + Dsui_0087 0.89 +0.9 86,801 - -3.1 86,825 - -2.5 86,833 + +0.9 86,879 + -1.7 86,919 + +0.9 86,929 + +0.9 86,929 + -0.7 86,937 - -0.2 86,973 + +0.9 87,016 + -1.1 87,017 - -1.0 87,021 + +0.9 87,040 - +0.0 87,040 - +0.0 87,056 + Dsui_0088 0.30 +0.9 87,064 - Dsui_0088 0.41 -0.2 87,087 + Dsui_0088 0.71 -0.1 87,094 - Dsui_0088 0.80 +0.1 87,136 + -0.1 87,136 + -1.1 87,136 + +0.9 87,136 + -0.1 87,136 + -1.1 87,136 + +0.9 87,144 - +0.9 87,144 - +0.1 87,180 - +0.2 87,237 + -1.7 87,250 - +0.7 87,278 - +1.1 87,284 - -0.1 87,323 - -0.0 87,330 + Dsui_0089 0.10 +0.9 87,340 + Dsui_0089 0.11 -0.7 87,343 - Dsui_0089 0.11 -0.8 87,402 + Dsui_0089 0.15 +0.9 87,490 + Dsui_0089 0.20 -1.5 87,549 - Dsui_0089 0.24 -0.0 87,564 - Dsui_0089 0.24 +1.0 87,587 - Dsui_0089 0.26 +0.9 87,768 + Dsui_0089 0.37 -0.1 87,783 + Dsui_0089 0.38 -0.1 87,818 + Dsui_0089 0.40 -0.1
Or see this region's nucleotide sequence