Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0078 and Dsui_0079 are separated by 50 nucleotides Dsui_0079 and Dsui_0080 are separated by 515 nucleotides
Dsui_0078: Dsui_0078 - putative dehydrogenase, at 74,640 to 75,620
_0078
Dsui_0079: Dsui_0079 - pseudaminic acid synthase, at 75,671 to 76,726
_0079
Dsui_0080: Dsui_0080 - pseudaminic acid biosynthesis-associated protein PseG, at 77,242 to 78,381
_0080
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 74.680 kb on + strand at 74.688 kb on - strand at 74.719 kb on - strand at 74.721 kb on + strand at 74.739 kb on - strand, within Dsui_0078 at 74.799 kb on + strand, within Dsui_0078 at 74.799 kb on + strand, within Dsui_0078 at 74.799 kb on + strand, within Dsui_0078 at 74.799 kb on + strand, within Dsui_0078 at 74.799 kb on + strand, within Dsui_0078 at 74.799 kb on + strand, within Dsui_0078 at 74.799 kb on + strand, within Dsui_0078 at 74.807 kb on + strand, within Dsui_0078 at 74.807 kb on - strand, within Dsui_0078 at 74.807 kb on - strand, within Dsui_0078 at 74.870 kb on - strand, within Dsui_0078 at 74.903 kb on + strand, within Dsui_0078 at 74.903 kb on + strand, within Dsui_0078 at 74.916 kb on - strand, within Dsui_0078 at 74.916 kb on - strand, within Dsui_0078 at 74.920 kb on + strand, within Dsui_0078 at 74.928 kb on - strand, within Dsui_0078 at 74.930 kb on + strand, within Dsui_0078 at 74.978 kb on - strand, within Dsui_0078 at 75.012 kb on + strand, within Dsui_0078 at 75.019 kb on + strand, within Dsui_0078 at 75.049 kb on + strand, within Dsui_0078 at 75.082 kb on + strand, within Dsui_0078 at 75.082 kb on + strand, within Dsui_0078 at 75.082 kb on + strand, within Dsui_0078 at 75.090 kb on - strand, within Dsui_0078 at 75.090 kb on - strand, within Dsui_0078 at 75.090 kb on - strand, within Dsui_0078 at 75.118 kb on + strand, within Dsui_0078 at 75.126 kb on - strand, within Dsui_0078 at 75.126 kb on - strand, within Dsui_0078 at 75.126 kb on - strand, within Dsui_0078 at 75.126 kb on - strand, within Dsui_0078 at 75.164 kb on + strand, within Dsui_0078 at 75.164 kb on + strand, within Dsui_0078 at 75.232 kb on - strand, within Dsui_0078 at 75.251 kb on - strand, within Dsui_0078 at 75.309 kb on + strand, within Dsui_0078 at 75.360 kb on + strand, within Dsui_0078 at 75.401 kb on + strand, within Dsui_0078 at 75.464 kb on - strand, within Dsui_0078 at 75.538 kb on - strand at 75.557 kb on - strand at 75.573 kb on - strand at 75.599 kb on - strand at 75.630 kb on + strand at 75.635 kb on + strand at 75.646 kb on + strand at 75.646 kb on + strand at 75.673 kb on + strand at 75.687 kb on + strand at 75.692 kb on + strand at 75.731 kb on + strand at 75.743 kb on + strand at 75.749 kb on - strand at 75.760 kb on - strand at 75.853 kb on + strand, within Dsui_0079 at 75.907 kb on - strand, within Dsui_0079 at 75.907 kb on - strand, within Dsui_0079 at 75.927 kb on + strand at 75.935 kb on - strand, within Dsui_0079 at 75.935 kb on - strand, within Dsui_0079 at 75.935 kb on - strand, within Dsui_0079 at 75.994 kb on + strand, within Dsui_0079 at 75.999 kb on + strand, within Dsui_0079 at 76.051 kb on - strand, within Dsui_0079 at 76.064 kb on - strand, within Dsui_0079 at 76.083 kb on + strand, within Dsui_0079 at 76.129 kb on + strand, within Dsui_0079 at 76.138 kb on - strand, within Dsui_0079 at 76.155 kb on - strand, within Dsui_0079 at 76.386 kb on + strand, within Dsui_0079 at 76.427 kb on - strand, within Dsui_0079 at 76.427 kb on - strand, within Dsui_0079 at 76.479 kb on - strand, within Dsui_0079 at 76.479 kb on - strand, within Dsui_0079 at 76.511 kb on + strand, within Dsui_0079 at 76.544 kb on + strand, within Dsui_0079 at 76.545 kb on - strand, within Dsui_0079 at 76.545 kb on - strand, within Dsui_0079 at 76.554 kb on - strand, within Dsui_0079 at 76.581 kb on + strand, within Dsui_0079 at 76.589 kb on - strand, within Dsui_0079 at 76.597 kb on - strand, within Dsui_0079 at 76.608 kb on - strand, within Dsui_0079 at 76.644 kb on + strand at 76.670 kb on - strand at 76.684 kb on - strand at 76.727 kb on + strand at 76.735 kb on - strand at 76.735 kb on - strand at 76.735 kb on - strand at 76.739 kb on + strand at 76.744 kb on + strand at 76.750 kb on + strand at 76.755 kb on + strand at 76.758 kb on - strand at 76.763 kb on - strand at 76.763 kb on - strand at 76.763 kb on - strand at 76.777 kb on - strand at 76.902 kb on - strand at 77.267 kb on - strand at 77.324 kb on + strand at 77.324 kb on + strand at 77.324 kb on + strand at 77.324 kb on + strand at 77.332 kb on - strand at 77.334 kb on + strand at 77.417 kb on - strand, within Dsui_0080 at 77.506 kb on + strand, within Dsui_0080 at 77.523 kb on + strand, within Dsui_0080 at 77.549 kb on - strand, within Dsui_0080 at 77.555 kb on + strand, within Dsui_0080 at 77.563 kb on - strand, within Dsui_0080 at 77.563 kb on - strand, within Dsui_0080 at 77.572 kb on - strand, within Dsui_0080 at 77.580 kb on + strand, within Dsui_0080 at 77.580 kb on + strand, within Dsui_0080 at 77.580 kb on + strand, within Dsui_0080 at 77.580 kb on + strand, within Dsui_0080 at 77.588 kb on - strand, within Dsui_0080 at 77.611 kb on - strand, within Dsui_0080 at 77.693 kb on + strand, within Dsui_0080 at 77.701 kb on - strand, within Dsui_0080 at 77.708 kb on + strand, within Dsui_0080 at 77.716 kb on - strand, within Dsui_0080 at 77.719 kb on + strand, within Dsui_0080
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 74,680 + -0.7 74,688 - -1.7 74,719 - -4.0 74,721 + -0.7 74,739 - Dsui_0078 0.10 -3.5 74,799 + Dsui_0078 0.16 -0.7 74,799 + Dsui_0078 0.16 +0.9 74,799 + Dsui_0078 0.16 -1.5 74,799 + Dsui_0078 0.16 +0.9 74,799 + Dsui_0078 0.16 -1.5 74,799 + Dsui_0078 0.16 +0.9 74,799 + Dsui_0078 0.16 -0.7 74,807 + Dsui_0078 0.17 +0.9 74,807 - Dsui_0078 0.17 -4.2 74,807 - Dsui_0078 0.17 -3.4 74,870 - Dsui_0078 0.23 -3.5 74,903 + Dsui_0078 0.27 -0.1 74,903 + Dsui_0078 0.27 -1.1 74,916 - Dsui_0078 0.28 -3.3 74,916 - Dsui_0078 0.28 -2.7 74,920 + Dsui_0078 0.29 -1.1 74,928 - Dsui_0078 0.29 -3.1 74,930 + Dsui_0078 0.30 +0.9 74,978 - Dsui_0078 0.34 -3.3 75,012 + Dsui_0078 0.38 -2.3 75,019 + Dsui_0078 0.39 -3.0 75,049 + Dsui_0078 0.42 +0.9 75,082 + Dsui_0078 0.45 +0.9 75,082 + Dsui_0078 0.45 -1.7 75,082 + Dsui_0078 0.45 -1.1 75,090 - Dsui_0078 0.46 -3.5 75,090 - Dsui_0078 0.46 -3.7 75,090 - Dsui_0078 0.46 -3.3 75,118 + Dsui_0078 0.49 +0.9 75,126 - Dsui_0078 0.50 -3.5 75,126 - Dsui_0078 0.50 -3.3 75,126 - Dsui_0078 0.50 -3.4 75,126 - Dsui_0078 0.50 -3.8 75,164 + Dsui_0078 0.53 +0.9 75,164 + Dsui_0078 0.53 -0.7 75,232 - Dsui_0078 0.60 -4.0 75,251 - Dsui_0078 0.62 -3.4 75,309 + Dsui_0078 0.68 -1.1 75,360 + Dsui_0078 0.73 +0.9 75,401 + Dsui_0078 0.78 +0.9 75,464 - Dsui_0078 0.84 -3.6 75,538 - -3.2 75,557 - -3.2 75,573 - -2.9 75,599 - -0.6 75,630 + +0.9 75,635 + +0.9 75,646 + +0.9 75,646 + -2.1 75,673 + -0.1 75,687 + -0.1 75,692 + -1.7 75,731 + -0.1 75,743 + +0.9 75,749 - -3.4 75,760 - -2.6 75,853 + Dsui_0079 0.17 -0.1 75,907 - Dsui_0079 0.22 -3.3 75,907 - Dsui_0079 0.22 -5.9 75,927 + +0.9 75,935 - Dsui_0079 0.25 -3.6 75,935 - Dsui_0079 0.25 -3.2 75,935 - Dsui_0079 0.25 -3.0 75,994 + Dsui_0079 0.31 +0.9 75,999 + Dsui_0079 0.31 -0.1 76,051 - Dsui_0079 0.36 -3.2 76,064 - Dsui_0079 0.37 -2.6 76,083 + Dsui_0079 0.39 +0.9 76,129 + Dsui_0079 0.43 +0.9 76,138 - Dsui_0079 0.44 -3.6 76,155 - Dsui_0079 0.46 -3.3 76,386 + Dsui_0079 0.68 +0.9 76,427 - Dsui_0079 0.72 -3.7 76,427 - Dsui_0079 0.72 -2.8 76,479 - Dsui_0079 0.77 -3.2 76,479 - Dsui_0079 0.77 -3.3 76,511 + Dsui_0079 0.80 -0.1 76,544 + Dsui_0079 0.83 +0.9 76,545 - Dsui_0079 0.83 -2.3 76,545 - Dsui_0079 0.83 -3.5 76,554 - Dsui_0079 0.84 -2.8 76,581 + Dsui_0079 0.86 +0.9 76,589 - Dsui_0079 0.87 -2.6 76,597 - Dsui_0079 0.88 -2.7 76,608 - Dsui_0079 0.89 -3.8 76,644 + -1.1 76,670 - -3.3 76,684 - -3.6 76,727 + -0.7 76,735 - -0.2 76,735 - +0.1 76,735 - -0.1 76,739 + +0.9 76,744 + -0.1 76,750 + -1.5 76,755 + +0.9 76,758 - +0.1 76,763 - -2.1 76,763 - -2.4 76,763 - -2.5 76,777 - -0.1 76,902 - -0.7 77,267 - -0.1 77,324 + -0.7 77,324 + +0.9 77,324 + +0.9 77,324 + +0.9 77,332 - -2.3 77,334 + +0.9 77,417 - Dsui_0080 0.15 -2.5 77,506 + Dsui_0080 0.23 +0.9 77,523 + Dsui_0080 0.25 +0.9 77,549 - Dsui_0080 0.27 -2.7 77,555 + Dsui_0080 0.27 +0.9 77,563 - Dsui_0080 0.28 -2.9 77,563 - Dsui_0080 0.28 -2.0 77,572 - Dsui_0080 0.29 -2.7 77,580 + Dsui_0080 0.30 +0.9 77,580 + Dsui_0080 0.30 +0.9 77,580 + Dsui_0080 0.30 -0.1 77,580 + Dsui_0080 0.30 +0.9 77,588 - Dsui_0080 0.30 -2.9 77,611 - Dsui_0080 0.32 -2.6 77,693 + Dsui_0080 0.40 +0.9 77,701 - Dsui_0080 0.40 -3.0 77,708 + Dsui_0080 0.41 -1.7 77,716 - Dsui_0080 0.42 -2.1 77,719 + Dsui_0080 0.42 +0.9
Or see this region's nucleotide sequence