Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0053 and Dsui_0054 are separated by 11 nucleotides Dsui_0054 and Dsui_0055 overlap by 4 nucleotides Dsui_0055 and Dsui_0056 overlap by 14 nucleotides Dsui_0056 and Dsui_0057 overlap by 4 nucleotides
Dsui_0053: Dsui_0053 - hypothetical protein, at 50,925 to 51,629
_0053
Dsui_0054: Dsui_0054 - glycosyl transferase possibly involved in lipopolysaccharide synthesis, at 51,641 to 52,201
_0054
Dsui_0055: Dsui_0055 - glycosyltransferase, at 52,198 to 53,385
_0055
Dsui_0056: Dsui_0056 - glycosyl transferase, at 53,372 to 54,301
_0056
Dsui_0057: Dsui_0057 - glycosyltransferase, at 54,298 to 55,488
_0057
Position (kb) 52
53
54 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 51.495 kb on + strand, within Dsui_0053 at 51.541 kb on - strand, within Dsui_0053 at 51.580 kb on - strand at 51.580 kb on - strand at 51.583 kb on + strand at 51.591 kb on - strand at 52.211 kb on + strand at 52.399 kb on - strand, within Dsui_0055 at 52.578 kb on - strand, within Dsui_0055 at 52.583 kb on - strand, within Dsui_0055 at 52.607 kb on + strand, within Dsui_0055 at 52.685 kb on + strand, within Dsui_0055 at 52.826 kb on - strand, within Dsui_0055 at 52.865 kb on + strand, within Dsui_0055 at 52.870 kb on + strand at 52.958 kb on + strand at 52.966 kb on - strand, within Dsui_0055 at 52.994 kb on + strand, within Dsui_0055 at 52.994 kb on + strand at 52.994 kb on + strand, within Dsui_0055 at 53.002 kb on - strand, within Dsui_0055 at 53.160 kb on + strand at 53.173 kb on - strand, within Dsui_0055 at 53.214 kb on - strand, within Dsui_0055 at 53.223 kb on + strand, within Dsui_0055 at 53.250 kb on + strand, within Dsui_0055 at 53.296 kb on - strand at 53.499 kb on - strand, within Dsui_0056 at 53.557 kb on - strand, within Dsui_0056 at 53.593 kb on - strand, within Dsui_0056 at 53.637 kb on - strand, within Dsui_0056 at 53.650 kb on + strand at 53.712 kb on - strand, within Dsui_0056 at 53.770 kb on - strand, within Dsui_0056 at 53.790 kb on + strand, within Dsui_0056 at 53.829 kb on + strand, within Dsui_0056 at 53.952 kb on - strand, within Dsui_0056 at 54.093 kb on - strand, within Dsui_0056 at 54.093 kb on - strand, within Dsui_0056 at 54.279 kb on + strand at 54.340 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 51,495 + Dsui_0053 0.81 +0.9 51,541 - Dsui_0053 0.87 +2.0 51,580 - -1.0 51,580 - +0.1 51,583 + +0.9 51,591 - +1.2 52,211 + -0.1 52,399 - Dsui_0055 0.17 -1.7 52,578 - Dsui_0055 0.32 -3.5 52,583 - Dsui_0055 0.32 -0.8 52,607 + Dsui_0055 0.34 -1.5 52,685 + Dsui_0055 0.41 -0.1 52,826 - Dsui_0055 0.53 -2.7 52,865 + Dsui_0055 0.56 +0.9 52,870 + +0.9 52,958 + +0.9 52,966 - Dsui_0055 0.65 -3.3 52,994 + Dsui_0055 0.67 +0.9 52,994 + +0.9 52,994 + Dsui_0055 0.67 -0.7 53,002 - Dsui_0055 0.68 -0.4 53,160 + +0.9 53,173 - Dsui_0055 0.82 -0.7 53,214 - Dsui_0055 0.86 -3.3 53,223 + Dsui_0055 0.86 +0.9 53,250 + Dsui_0055 0.89 -0.1 53,296 - -1.9 53,499 - Dsui_0056 0.14 -2.3 53,557 - Dsui_0056 0.20 -1.6 53,593 - Dsui_0056 0.24 -1.6 53,637 - Dsui_0056 0.28 -4.8 53,650 + -0.1 53,712 - Dsui_0056 0.37 -2.6 53,770 - Dsui_0056 0.43 -2.5 53,790 + Dsui_0056 0.45 +0.9 53,829 + Dsui_0056 0.49 -0.1 53,952 - Dsui_0056 0.62 -3.6 54,093 - Dsui_0056 0.78 -1.4 54,093 - Dsui_0056 0.78 -4.4 54,279 + -0.1 54,340 - +0.2
Or see this region's nucleotide sequence