Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0022 and Dsui_0023 are separated by 245 nucleotides Dsui_0023 and Dsui_0024 are separated by 153 nucleotides Dsui_0024 and Dsui_0025 are separated by 250 nucleotides
Dsui_0022: Dsui_0022 - hypothetical protein, at 19,403 to 20,341
_0022
Dsui_0023: Dsui_0023 - acetylornithine/succinylornithine aminotransferase, at 20,587 to 21,777
_0023
Dsui_0024: Dsui_0024 - cytochrome c556, at 21,931 to 22,377
_0024
Dsui_0025: Dsui_0025 - hypothetical protein, at 22,628 to 23,710
_0025
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 19.592 kb on - strand at 19.700 kb on + strand, within Dsui_0022 at 19.702 kb on - strand, within Dsui_0022 at 19.723 kb on + strand, within Dsui_0022 at 19.775 kb on - strand at 19.818 kb on + strand, within Dsui_0022 at 19.897 kb on + strand, within Dsui_0022 at 19.905 kb on - strand, within Dsui_0022 at 19.981 kb on + strand, within Dsui_0022 at 20.059 kb on - strand at 20.086 kb on - strand, within Dsui_0022 at 20.105 kb on - strand, within Dsui_0022 at 20.145 kb on - strand, within Dsui_0022 at 20.177 kb on - strand at 20.293 kb on - strand at 20.316 kb on + strand at 20.324 kb on - strand at 20.383 kb on + strand at 20.528 kb on + strand at 20.529 kb on - strand at 20.540 kb on + strand at 20.603 kb on + strand at 20.628 kb on + strand at 20.705 kb on - strand at 20.719 kb on + strand, within Dsui_0023 at 20.736 kb on + strand, within Dsui_0023 at 20.799 kb on + strand, within Dsui_0023 at 20.921 kb on + strand, within Dsui_0023 at 20.974 kb on + strand, within Dsui_0023 at 21.010 kb on - strand, within Dsui_0023 at 21.079 kb on + strand at 21.181 kb on - strand at 21.186 kb on - strand at 21.221 kb on + strand at 21.227 kb on + strand, within Dsui_0023 at 21.286 kb on - strand, within Dsui_0023 at 21.360 kb on + strand at 21.584 kb on - strand at 21.779 kb on - strand at 21.779 kb on - strand at 21.783 kb on + strand at 21.795 kb on - strand at 21.859 kb on - strand at 21.900 kb on + strand at 22.049 kb on - strand at 22.062 kb on + strand, within Dsui_0024 at 22.212 kb on + strand, within Dsui_0024 at 22.212 kb on + strand, within Dsui_0024 at 22.212 kb on + strand, within Dsui_0024 at 22.220 kb on - strand, within Dsui_0024 at 22.220 kb on - strand at 22.221 kb on - strand at 22.223 kb on + strand, within Dsui_0024 at 22.364 kb on - strand at 22.390 kb on + strand at 22.410 kb on + strand at 22.468 kb on - strand at 22.655 kb on + strand at 22.751 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 19,592 - +0.9 19,700 + Dsui_0022 0.32 +0.9 19,702 - Dsui_0022 0.32 -2.1 19,723 + Dsui_0022 0.34 +0.9 19,775 - +0.9 19,818 + Dsui_0022 0.44 +0.1 19,897 + Dsui_0022 0.53 +1.3 19,905 - Dsui_0022 0.53 +0.9 19,981 + Dsui_0022 0.62 -2.3 20,059 - +0.9 20,086 - Dsui_0022 0.73 -1.1 20,105 - Dsui_0022 0.75 -0.7 20,145 - Dsui_0022 0.79 -0.1 20,177 - +0.9 20,293 - +0.9 20,316 + -0.7 20,324 - +0.9 20,383 + -1.5 20,528 + -0.1 20,529 - -0.7 20,540 + +0.5 20,603 + +0.9 20,628 + +0.9 20,705 - +0.9 20,719 + Dsui_0023 0.11 -2.5 20,736 + Dsui_0023 0.13 -1.7 20,799 + Dsui_0023 0.18 -0.1 20,921 + Dsui_0023 0.28 -0.7 20,974 + Dsui_0023 0.32 -2.5 21,010 - Dsui_0023 0.36 -1.8 21,079 + +0.9 21,181 - +0.9 21,186 - +0.9 21,221 + +0.9 21,227 + Dsui_0023 0.54 -0.7 21,286 - Dsui_0023 0.59 -1.5 21,360 + +0.9 21,584 - +0.9 21,779 - -0.7 21,779 - +0.9 21,783 + -0.1 21,795 - -1.1 21,859 - +0.9 21,900 + -0.9 22,049 - -0.1 22,062 + Dsui_0024 0.29 +3.6 22,212 + Dsui_0024 0.63 +3.4 22,212 + Dsui_0024 0.63 +2.8 22,212 + Dsui_0024 0.63 +3.6 22,220 - Dsui_0024 0.65 -0.7 22,220 - -0.1 22,221 - +0.9 22,223 + Dsui_0024 0.65 +3.5 22,364 - -0.7 22,390 + -0.9 22,410 + +0.1 22,468 - -1.1 22,655 + -0.1 22,751 + +0.9
Or see this region's nucleotide sequence