Experiment: ALP with Fusidic 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0003 and Dsui_0004 are separated by 42 nucleotides Dsui_0004 and Dsui_0005 are separated by 32 nucleotides Dsui_0005 and Dsui_0006 are separated by 9 nucleotides Dsui_0006 and Dsui_0007 are separated by 85 nucleotides
Dsui_0003: Dsui_0003 - putative Zn-dependent protease, at 2,299 to 3,786
_0003
Dsui_0004: Dsui_0004 - molybdenum cofactor biosynthesis protein MoaC, at 3,829 to 4,323
_0004
Dsui_0005: Dsui_0005 - putative secreted protein, at 4,356 to 4,826
_0005
Dsui_0006: Dsui_0006 - sulfur oxidation protein SoxZ, at 4,836 to 5,153
_0006
Dsui_0007: Dsui_0007 - cold shock protein, at 5,239 to 5,442
_0007
Position (kb)
3
4
5 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2.977 kb on - strand, within Dsui_0003 at 3.026 kb on - strand, within Dsui_0003 at 3.131 kb on - strand at 3.222 kb on - strand, within Dsui_0003 at 3.413 kb on - strand, within Dsui_0003 at 3.503 kb on - strand, within Dsui_0003 at 3.503 kb on - strand at 3.503 kb on - strand, within Dsui_0003 at 3.567 kb on - strand, within Dsui_0003 at 3.791 kb on + strand at 3.833 kb on - strand at 3.833 kb on - strand at 3.861 kb on - strand at 4.008 kb on + strand, within Dsui_0004 at 4.035 kb on + strand, within Dsui_0004 at 4.079 kb on + strand, within Dsui_0004 at 4.093 kb on - strand at 4.099 kb on + strand, within Dsui_0004 at 4.205 kb on + strand, within Dsui_0004 at 4.230 kb on - strand, within Dsui_0004 at 4.241 kb on - strand at 4.260 kb on + strand, within Dsui_0004 at 4.426 kb on - strand, within Dsui_0005 at 4.426 kb on - strand at 4.504 kb on - strand, within Dsui_0005 at 4.522 kb on - strand, within Dsui_0005 at 4.630 kb on + strand, within Dsui_0005 at 4.631 kb on - strand, within Dsui_0005 at 4.695 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.713 kb on - strand, within Dsui_0005 at 4.725 kb on - strand, within Dsui_0005 at 4.742 kb on + strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.762 kb on - strand at 4.861 kb on + strand at 4.887 kb on + strand, within Dsui_0006 at 4.893 kb on - strand, within Dsui_0006 at 4.910 kb on + strand, within Dsui_0006 at 5.002 kb on + strand, within Dsui_0006 at 5.002 kb on - strand, within Dsui_0006 at 5.007 kb on + strand, within Dsui_0006 at 5.007 kb on + strand, within Dsui_0006 at 5.026 kb on - strand, within Dsui_0006 at 5.077 kb on - strand at 5.123 kb on - strand at 5.123 kb on - strand at 5.164 kb on + strand at 5.172 kb on - strand at 5.172 kb on - strand at 5.172 kb on - strand at 5.173 kb on - strand at 5.237 kb on + strand at 5.237 kb on + strand at 5.237 kb on + strand at 5.244 kb on - strand at 5.244 kb on - strand at 5.244 kb on - strand at 5.297 kb on - strand, within Dsui_0007
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.007 mg/ml remove 2,977 - Dsui_0003 0.46 -2.5 3,026 - Dsui_0003 0.49 -2.1 3,131 - -0.1 3,222 - Dsui_0003 0.62 -3.7 3,413 - Dsui_0003 0.75 -0.7 3,503 - Dsui_0003 0.81 -2.6 3,503 - -0.1 3,503 - Dsui_0003 0.81 -2.3 3,567 - Dsui_0003 0.85 -3.0 3,791 + -0.4 3,833 - -0.1 3,833 - -0.7 3,861 - -1.1 4,008 + Dsui_0004 0.36 +0.2 4,035 + Dsui_0004 0.42 +0.8 4,079 + Dsui_0004 0.51 +0.3 4,093 - -0.1 4,099 + Dsui_0004 0.55 +0.7 4,205 + Dsui_0004 0.76 +0.3 4,230 - Dsui_0004 0.81 -1.7 4,241 - +0.9 4,260 + Dsui_0004 0.87 +0.2 4,426 - Dsui_0005 0.15 -0.1 4,426 - +0.9 4,504 - Dsui_0005 0.31 +0.9 4,522 - Dsui_0005 0.35 -0.1 4,630 + Dsui_0005 0.58 -0.3 4,631 - Dsui_0005 0.58 +0.9 4,695 + Dsui_0005 0.72 +0.2 4,705 + Dsui_0005 0.74 -0.0 4,705 + Dsui_0005 0.74 +0.1 4,705 + Dsui_0005 0.74 +0.2 4,705 + Dsui_0005 0.74 +0.0 4,713 - Dsui_0005 0.76 -0.1 4,725 - Dsui_0005 0.78 +0.9 4,742 + Dsui_0005 0.82 -0.3 4,750 - Dsui_0005 0.84 +0.9 4,750 - Dsui_0005 0.84 +0.9 4,750 - Dsui_0005 0.84 -0.7 4,750 - Dsui_0005 0.84 -1.5 4,762 - +0.9 4,861 + +0.5 4,887 + Dsui_0006 0.16 -0.3 4,893 - Dsui_0006 0.18 -0.1 4,910 + Dsui_0006 0.23 +0.9 5,002 + Dsui_0006 0.52 -0.0 5,002 - Dsui_0006 0.52 -0.7 5,007 + Dsui_0006 0.54 -0.2 5,007 + Dsui_0006 0.54 -0.3 5,026 - Dsui_0006 0.60 -0.1 5,077 - +0.9 5,123 - +0.9 5,123 - +0.9 5,164 + -0.2 5,172 - +0.9 5,172 - -2.3 5,172 - +1.9 5,173 - -1.1 5,237 + +0.9 5,237 + +0.9 5,237 + +0.9 5,244 - +0.5 5,244 - -0.5 5,244 - -0.2 5,297 - Dsui_0007 0.28 +0.5
Or see this region's nucleotide sequence