Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3316 and Dsui_3317 are separated by 105 nucleotides Dsui_3317 and Dsui_3318 are separated by 88 nucleotides Dsui_3318 and Dsui_3319 are separated by 226 nucleotides
Dsui_3316: Dsui_3316 - Gram-negative bacterial tonB protein, at 3,556,516 to 3,557,289
_3316
Dsui_3317: Dsui_3317 - cytochrome c551/c552, at 3,557,395 to 3,557,733
_3317
Dsui_3318: Dsui_3318 - cytochrome c, mono- and diheme variants family, at 3,557,822 to 3,559,462
_3318
Dsui_3319: Dsui_3319 - uroporphyrin-III C-methyltransferase, at 3,559,689 to 3,560,486
_3319
Position (kb)
3557
3558
3559
3560 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3556.845 kb on - strand, within Dsui_3316 at 3556.964 kb on + strand, within Dsui_3316 at 3556.972 kb on - strand, within Dsui_3316 at 3557.093 kb on + strand at 3557.116 kb on - strand, within Dsui_3316 at 3557.177 kb on - strand, within Dsui_3316 at 3557.187 kb on - strand, within Dsui_3316 at 3557.239 kb on + strand at 3557.294 kb on + strand at 3557.506 kb on - strand at 3557.506 kb on - strand, within Dsui_3317 at 3557.705 kb on - strand at 3557.705 kb on - strand at 3557.707 kb on + strand at 3557.715 kb on - strand at 3557.833 kb on - strand at 3557.837 kb on - strand at 3558.028 kb on - strand, within Dsui_3318 at 3558.028 kb on - strand, within Dsui_3318 at 3558.035 kb on - strand, within Dsui_3318 at 3558.070 kb on + strand, within Dsui_3318 at 3558.070 kb on + strand at 3558.075 kb on + strand, within Dsui_3318 at 3558.078 kb on - strand, within Dsui_3318 at 3558.082 kb on - strand, within Dsui_3318 at 3558.095 kb on - strand, within Dsui_3318 at 3558.113 kb on - strand, within Dsui_3318 at 3558.189 kb on + strand at 3558.304 kb on + strand, within Dsui_3318 at 3558.312 kb on - strand, within Dsui_3318 at 3558.371 kb on - strand, within Dsui_3318 at 3558.448 kb on - strand, within Dsui_3318 at 3558.466 kb on + strand at 3558.563 kb on - strand, within Dsui_3318 at 3558.573 kb on - strand, within Dsui_3318 at 3558.605 kb on - strand, within Dsui_3318 at 3558.683 kb on - strand at 3558.689 kb on - strand, within Dsui_3318 at 3558.707 kb on + strand, within Dsui_3318 at 3558.727 kb on - strand, within Dsui_3318 at 3558.768 kb on + strand at 3558.859 kb on + strand, within Dsui_3318 at 3558.959 kb on - strand, within Dsui_3318 at 3558.959 kb on - strand, within Dsui_3318 at 3559.006 kb on + strand at 3559.014 kb on - strand, within Dsui_3318 at 3559.073 kb on + strand at 3559.146 kb on - strand, within Dsui_3318 at 3559.160 kb on - strand, within Dsui_3318 at 3559.170 kb on + strand, within Dsui_3318 at 3559.249 kb on - strand, within Dsui_3318 at 3559.260 kb on - strand, within Dsui_3318 at 3559.294 kb on - strand, within Dsui_3318 at 3559.301 kb on - strand at 3559.301 kb on - strand at 3559.327 kb on + strand at 3559.340 kb on - strand at 3559.340 kb on - strand at 3559.393 kb on - strand at 3559.417 kb on - strand at 3559.463 kb on + strand at 3559.476 kb on - strand at 3559.504 kb on - strand at 3559.504 kb on - strand at 3559.511 kb on - strand at 3559.533 kb on - strand at 3559.558 kb on - strand at 3559.607 kb on - strand at 3559.646 kb on + strand at 3559.651 kb on + strand at 3559.717 kb on + strand at 3559.719 kb on - strand at 3559.725 kb on - strand at 3559.888 kb on - strand at 3559.899 kb on - strand, within Dsui_3319 at 3559.906 kb on + strand, within Dsui_3319 at 3560.122 kb on + strand, within Dsui_3319 at 3560.196 kb on + strand, within Dsui_3319 at 3560.196 kb on + strand, within Dsui_3319 at 3560.204 kb on - strand at 3560.318 kb on - strand at 3560.323 kb on + strand, within Dsui_3319 at 3560.338 kb on + strand, within Dsui_3319 at 3560.342 kb on + strand, within Dsui_3319
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 3,556,845 - Dsui_3316 0.43 -0.8 3,556,964 + Dsui_3316 0.58 -0.7 3,556,972 - Dsui_3316 0.59 -0.6 3,557,093 + +0.3 3,557,116 - Dsui_3316 0.78 -0.3 3,557,177 - Dsui_3316 0.85 -0.4 3,557,187 - Dsui_3316 0.87 +0.0 3,557,239 + -2.5 3,557,294 + +0.3 3,557,506 - +0.3 3,557,506 - Dsui_3317 0.33 -0.7 3,557,705 - -1.3 3,557,705 - +0.3 3,557,707 + +0.3 3,557,715 - -2.0 3,557,833 - +0.3 3,557,837 - -2.7 3,558,028 - Dsui_3318 0.13 -1.7 3,558,028 - Dsui_3318 0.13 -2.7 3,558,035 - Dsui_3318 0.13 -1.3 3,558,070 + Dsui_3318 0.15 +0.3 3,558,070 + +0.3 3,558,075 + Dsui_3318 0.15 -0.7 3,558,078 - Dsui_3318 0.16 -1.3 3,558,082 - Dsui_3318 0.16 -1.3 3,558,095 - Dsui_3318 0.17 +0.3 3,558,113 - Dsui_3318 0.18 -1.5 3,558,189 + -2.0 3,558,304 + Dsui_3318 0.29 -1.7 3,558,312 - Dsui_3318 0.30 -0.7 3,558,371 - Dsui_3318 0.33 +0.3 3,558,448 - Dsui_3318 0.38 -1.3 3,558,466 + +0.3 3,558,563 - Dsui_3318 0.45 -0.7 3,558,573 - Dsui_3318 0.46 -1.7 3,558,605 - Dsui_3318 0.48 -1.7 3,558,683 - +0.3 3,558,689 - Dsui_3318 0.53 +0.3 3,558,707 + Dsui_3318 0.54 -1.7 3,558,727 - Dsui_3318 0.55 +0.3 3,558,768 + -0.7 3,558,859 + Dsui_3318 0.63 -0.7 3,558,959 - Dsui_3318 0.69 +0.3 3,558,959 - Dsui_3318 0.69 +0.3 3,559,006 + -1.3 3,559,014 - Dsui_3318 0.73 +0.3 3,559,073 + +0.3 3,559,146 - Dsui_3318 0.81 -1.3 3,559,160 - Dsui_3318 0.82 +0.3 3,559,170 + Dsui_3318 0.82 +0.3 3,559,249 - Dsui_3318 0.87 -1.5 3,559,260 - Dsui_3318 0.88 -3.0 3,559,294 - Dsui_3318 0.90 -3.8 3,559,301 - +0.3 3,559,301 - -2.3 3,559,327 + -1.3 3,559,340 - +0.3 3,559,340 - -1.3 3,559,393 - -0.7 3,559,417 - +0.3 3,559,463 + +0.3 3,559,476 - -3.0 3,559,504 - +0.3 3,559,504 - +0.3 3,559,511 - +0.3 3,559,533 - +0.3 3,559,558 - -1.3 3,559,607 - +0.3 3,559,646 + +0.3 3,559,651 + -0.7 3,559,717 + -0.7 3,559,719 - -0.7 3,559,725 - -2.3 3,559,888 - +0.3 3,559,899 - Dsui_3319 0.26 +0.3 3,559,906 + Dsui_3319 0.27 -0.9 3,560,122 + Dsui_3319 0.54 -2.0 3,560,196 + Dsui_3319 0.64 -3.4 3,560,196 + Dsui_3319 0.64 +0.3 3,560,204 - +0.3 3,560,318 - +0.3 3,560,323 + Dsui_3319 0.79 +1.9 3,560,338 + Dsui_3319 0.81 -2.0 3,560,342 + Dsui_3319 0.82 -1.4
Or see this region's nucleotide sequence