Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3101 and Dsui_3102 are separated by 62 nucleotides Dsui_3102 and Dsui_3103 are separated by 165 nucleotides Dsui_3103 and Dsui_3104 are separated by 16 nucleotides
Dsui_3101: Dsui_3101 - transcriptional regulator, at 3,319,234 to 3,320,133
_3101
Dsui_3102: Dsui_3102 - molybdopterin-guanine dinucleotide biosynthesis protein A, proteobacterial, at 3,320,196 to 3,320,792
_3102
Dsui_3103: Dsui_3103 - putative signal transduction protein containing EAL and modified HD-GYP domains, at 3,320,958 to 3,322,157
_3103
Dsui_3104: Dsui_3104 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 3,322,174 to 3,324,675
_3104
Position (kb)
3320
3321
3322
3323 Strain fitness (log2 ratio)
-2
-1
0
1 at 3320.041 kb on + strand, within Dsui_3101 at 3320.182 kb on - strand at 3320.205 kb on + strand at 3320.250 kb on + strand at 3320.357 kb on + strand, within Dsui_3102 at 3320.392 kb on - strand at 3320.404 kb on - strand at 3320.404 kb on - strand at 3320.570 kb on + strand, within Dsui_3102 at 3320.573 kb on - strand at 3320.683 kb on + strand, within Dsui_3102 at 3320.686 kb on + strand, within Dsui_3102 at 3320.840 kb on - strand at 3320.973 kb on - strand at 3320.973 kb on - strand at 3320.982 kb on - strand at 3321.027 kb on - strand at 3321.079 kb on + strand, within Dsui_3103 at 3321.089 kb on + strand, within Dsui_3103 at 3321.102 kb on + strand, within Dsui_3103 at 3321.110 kb on - strand at 3321.110 kb on - strand, within Dsui_3103 at 3321.112 kb on + strand, within Dsui_3103 at 3321.115 kb on - strand at 3321.136 kb on + strand, within Dsui_3103 at 3321.203 kb on + strand, within Dsui_3103 at 3321.319 kb on - strand, within Dsui_3103 at 3321.337 kb on - strand, within Dsui_3103 at 3321.359 kb on + strand, within Dsui_3103 at 3321.359 kb on + strand, within Dsui_3103 at 3321.372 kb on - strand, within Dsui_3103 at 3321.465 kb on + strand, within Dsui_3103 at 3321.519 kb on - strand, within Dsui_3103 at 3321.643 kb on - strand, within Dsui_3103 at 3321.682 kb on + strand, within Dsui_3103 at 3321.748 kb on + strand, within Dsui_3103 at 3321.796 kb on + strand, within Dsui_3103 at 3321.801 kb on + strand, within Dsui_3103 at 3321.929 kb on + strand, within Dsui_3103 at 3321.956 kb on + strand, within Dsui_3103 at 3322.043 kb on + strand at 3322.127 kb on + strand at 3322.140 kb on - strand at 3322.172 kb on - strand at 3322.209 kb on + strand at 3322.243 kb on + strand at 3322.606 kb on - strand, within Dsui_3104 at 3322.691 kb on + strand, within Dsui_3104 at 3322.699 kb on - strand, within Dsui_3104 at 3322.836 kb on + strand, within Dsui_3104 at 3322.981 kb on - strand, within Dsui_3104 at 3322.984 kb on - strand, within Dsui_3104 at 3323.011 kb on + strand, within Dsui_3104 at 3323.051 kb on - strand, within Dsui_3104 at 3323.123 kb on + strand, within Dsui_3104
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 3,320,041 + Dsui_3101 0.90 -0.2 3,320,182 - +0.3 3,320,205 + -0.8 3,320,250 + -0.5 3,320,357 + Dsui_3102 0.27 -0.3 3,320,392 - -0.7 3,320,404 - -0.7 3,320,404 - -0.7 3,320,570 + Dsui_3102 0.63 +0.1 3,320,573 - -1.3 3,320,683 + Dsui_3102 0.82 -1.0 3,320,686 + Dsui_3102 0.82 -0.9 3,320,840 - -0.7 3,320,973 - +0.3 3,320,973 - +0.3 3,320,982 - +0.3 3,321,027 - -2.5 3,321,079 + Dsui_3103 0.10 -0.0 3,321,089 + Dsui_3103 0.11 +0.4 3,321,102 + Dsui_3103 0.12 -0.2 3,321,110 - +0.3 3,321,110 - Dsui_3103 0.13 -1.3 3,321,112 + Dsui_3103 0.13 -0.0 3,321,115 - +0.3 3,321,136 + Dsui_3103 0.15 +0.1 3,321,203 + Dsui_3103 0.20 -0.1 3,321,319 - Dsui_3103 0.30 -1.3 3,321,337 - Dsui_3103 0.32 -1.7 3,321,359 + Dsui_3103 0.33 -0.1 3,321,359 + Dsui_3103 0.33 +0.0 3,321,372 - Dsui_3103 0.34 -0.7 3,321,465 + Dsui_3103 0.42 +0.1 3,321,519 - Dsui_3103 0.47 +0.3 3,321,643 - Dsui_3103 0.57 -1.3 3,321,682 + Dsui_3103 0.60 +0.0 3,321,748 + Dsui_3103 0.66 -0.0 3,321,796 + Dsui_3103 0.70 +0.2 3,321,801 + Dsui_3103 0.70 -0.0 3,321,929 + Dsui_3103 0.81 -0.0 3,321,956 + Dsui_3103 0.83 +0.1 3,322,043 + -0.3 3,322,127 + -0.0 3,322,140 - -2.0 3,322,172 - -0.7 3,322,209 + -0.0 3,322,243 + -0.3 3,322,606 - Dsui_3104 0.17 -1.7 3,322,691 + Dsui_3104 0.21 -0.6 3,322,699 - Dsui_3104 0.21 +0.3 3,322,836 + Dsui_3104 0.26 -0.1 3,322,981 - Dsui_3104 0.32 -1.3 3,322,984 - Dsui_3104 0.32 -2.3 3,323,011 + Dsui_3104 0.33 -0.1 3,323,051 - Dsui_3104 0.35 -1.7 3,323,123 + Dsui_3104 0.38 -0.4
Or see this region's nucleotide sequence