Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2497 and Dsui_2498 are separated by 16 nucleotides Dsui_2498 and Dsui_2499 overlap by 4 nucleotides Dsui_2499 and Dsui_2500 are separated by 52 nucleotides Dsui_2500 and Dsui_2501 are separated by 112 nucleotides
Dsui_2497: Dsui_2497 - mercuric reductase, at 2,645,125 to 2,646,771
_2497
Dsui_2498: Dsui_2498 - mercuric resistance transcriptional repressor protein MerD, at 2,646,788 to 2,647,153
_2498
Dsui_2499: Dsui_2499 - MerE protein, at 2,647,150 to 2,647,386
_2499
Dsui_2500: Dsui_2500 - site-specific recombinase, DNA invertase Pin, at 2,647,439 to 2,648,053
_2500
Dsui_2501: Dsui_2501 - TniQ protein, at 2,648,166 to 2,649,332
_2501
Position (kb)
2647
2648
2649 Strain fitness (log2 ratio)
-2
-1
0
1 at 2646.490 kb on - strand, within Dsui_2497 at 2646.803 kb on - strand at 2646.852 kb on + strand, within Dsui_2498 at 2646.876 kb on + strand, within Dsui_2498 at 2646.908 kb on - strand at 2646.908 kb on - strand, within Dsui_2498 at 2646.942 kb on - strand, within Dsui_2498 at 2646.979 kb on - strand, within Dsui_2498 at 2647.155 kb on - strand at 2647.222 kb on + strand, within Dsui_2499 at 2647.227 kb on + strand, within Dsui_2499 at 2647.235 kb on - strand, within Dsui_2499 at 2647.299 kb on + strand, within Dsui_2499 at 2647.307 kb on - strand at 2647.324 kb on - strand at 2647.474 kb on - strand at 2647.692 kb on + strand, within Dsui_2500 at 2647.748 kb on + strand, within Dsui_2500 at 2647.769 kb on + strand, within Dsui_2500 at 2647.777 kb on - strand, within Dsui_2500 at 2647.798 kb on + strand at 2647.800 kb on + strand at 2647.823 kb on - strand, within Dsui_2500 at 2648.008 kb on + strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.016 kb on - strand at 2648.231 kb on + strand at 2648.239 kb on - strand at 2648.352 kb on + strand, within Dsui_2501 at 2648.419 kb on + strand, within Dsui_2501 at 2648.495 kb on + strand, within Dsui_2501 at 2648.643 kb on - strand at 2648.643 kb on - strand, within Dsui_2501 at 2648.646 kb on + strand, within Dsui_2501
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 2,646,490 - Dsui_2497 0.83 -2.3 2,646,803 - -0.7 2,646,852 + Dsui_2498 0.17 +0.3 2,646,876 + Dsui_2498 0.24 -2.3 2,646,908 - +0.3 2,646,908 - Dsui_2498 0.33 +0.3 2,646,942 - Dsui_2498 0.42 -1.3 2,646,979 - Dsui_2498 0.52 -0.7 2,647,155 - -2.1 2,647,222 + Dsui_2499 0.30 +0.3 2,647,227 + Dsui_2499 0.32 -1.3 2,647,235 - Dsui_2499 0.36 +0.3 2,647,299 + Dsui_2499 0.63 +0.3 2,647,307 - +0.3 2,647,324 - +0.3 2,647,474 - -0.1 2,647,692 + Dsui_2500 0.41 -1.3 2,647,748 + Dsui_2500 0.50 +0.3 2,647,769 + Dsui_2500 0.54 +0.3 2,647,777 - Dsui_2500 0.55 +0.1 2,647,798 + +0.3 2,647,800 + +0.3 2,647,823 - Dsui_2500 0.62 +0.1 2,648,008 + -1.3 2,648,016 - +0.4 2,648,016 - +0.1 2,648,016 - -0.0 2,648,016 - +0.8 2,648,016 - +0.5 2,648,231 + -1.7 2,648,239 - +0.3 2,648,352 + Dsui_2501 0.16 +0.3 2,648,419 + Dsui_2501 0.22 -1.3 2,648,495 + Dsui_2501 0.28 +0.3 2,648,643 - +0.3 2,648,643 - Dsui_2501 0.41 +0.3 2,648,646 + Dsui_2501 0.41 -0.7
Or see this region's nucleotide sequence