Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2312 and Dsui_2313 are separated by 144 nucleotides Dsui_2313 and Dsui_2314 overlap by 4 nucleotides Dsui_2314 and Dsui_2315 overlap by 1 nucleotides
Dsui_2312: Dsui_2312 - 3-hydroxybutyrate dehydrogenase, at 2,467,804 to 2,468,586
_2312
Dsui_2313: Dsui_2313 - CTP synthase, at 2,468,731 to 2,470,371
_2313
Dsui_2314: Dsui_2314 - 3-deoxy-8-phosphooctulonate synthase, at 2,470,368 to 2,471,207
_2314
Dsui_2315: Dsui_2315 - Stress responsive A/B Barrel Domain-containing protein, at 2,471,207 to 2,471,500
_2315
Position (kb)
2468
2469
2470
2471 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2467.787 kb on + strand at 2468.130 kb on + strand, within Dsui_2312 at 2468.138 kb on + strand, within Dsui_2312 at 2468.287 kb on + strand, within Dsui_2312 at 2468.319 kb on - strand at 2468.336 kb on - strand at 2468.410 kb on + strand, within Dsui_2312 at 2468.410 kb on + strand at 2468.410 kb on + strand, within Dsui_2312 at 2468.410 kb on - strand at 2468.410 kb on - strand at 2468.425 kb on + strand, within Dsui_2312 at 2468.591 kb on + strand at 2468.596 kb on + strand at 2468.609 kb on - strand at 2468.720 kb on + strand at 2470.340 kb on + strand at 2471.171 kb on + strand at 2471.171 kb on + strand at 2471.176 kb on + strand at 2471.235 kb on - strand at 2471.238 kb on + strand, within Dsui_2315 at 2471.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 2,467,787 + -0.6 2,468,130 + Dsui_2312 0.42 -1.2 2,468,138 + Dsui_2312 0.43 -2.2 2,468,287 + Dsui_2312 0.62 -1.2 2,468,319 - +0.4 2,468,336 - -0.6 2,468,410 + Dsui_2312 0.77 -2.2 2,468,410 + +0.4 2,468,410 + Dsui_2312 0.77 -1.2 2,468,410 - +0.4 2,468,410 - -1.2 2,468,425 + Dsui_2312 0.79 -1.2 2,468,591 + +1.4 2,468,596 + -1.2 2,468,609 - -2.2 2,468,720 + -3.8 2,470,340 + -2.4 2,471,171 + -0.6 2,471,171 + +0.0 2,471,176 + +0.2 2,471,235 - -2.2 2,471,238 + Dsui_2315 0.11 +0.3 2,471,345 - +0.4
Or see this region's nucleotide sequence