Experiment: ALP with Bacitracin 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2039 and Dsui_2040 are separated by 26 nucleotides Dsui_2040 and Dsui_2041 are separated by 60 nucleotides Dsui_2041 and Dsui_2042 are separated by 20 nucleotides
Dsui_2039: Dsui_2039 - OAH/OAS sulfhydrylase, at 2,158,129 to 2,159,415
_2039
Dsui_2040: Dsui_2040 - putative CoA-binding protein, at 2,159,442 to 2,159,900
_2040
Dsui_2041: Dsui_2041 - enoyl-CoA hydratase/carnithine racemase, at 2,159,961 to 2,160,746
_2041
Dsui_2042: Dsui_2042 - cystathionine beta-lyase/cystathionine gamma-synthase, at 2,160,767 to 2,161,945
_2042
Position (kb)
2159
2160 Strain fitness (log2 ratio)
-2
-1
0
1 at 2158.447 kb on + strand, within Dsui_2039 at 2158.458 kb on + strand, within Dsui_2039 at 2158.490 kb on + strand, within Dsui_2039 at 2158.542 kb on + strand, within Dsui_2039 at 2158.558 kb on - strand, within Dsui_2039 at 2158.559 kb on - strand, within Dsui_2039 at 2158.617 kb on - strand, within Dsui_2039 at 2158.678 kb on + strand, within Dsui_2039 at 2158.691 kb on - strand, within Dsui_2039 at 2158.758 kb on + strand at 2158.781 kb on + strand at 2158.801 kb on + strand, within Dsui_2039 at 2158.814 kb on - strand, within Dsui_2039 at 2158.832 kb on + strand, within Dsui_2039 at 2158.842 kb on + strand, within Dsui_2039 at 2158.884 kb on - strand, within Dsui_2039 at 2158.932 kb on + strand, within Dsui_2039 at 2158.965 kb on + strand at 2158.973 kb on - strand, within Dsui_2039 at 2158.978 kb on - strand, within Dsui_2039 at 2159.225 kb on - strand, within Dsui_2039 at 2159.250 kb on - strand, within Dsui_2039 at 2159.260 kb on + strand, within Dsui_2039 at 2159.268 kb on - strand, within Dsui_2039 at 2159.419 kb on - strand at 2159.429 kb on - strand at 2159.450 kb on + strand at 2159.450 kb on + strand at 2159.458 kb on - strand at 2159.608 kb on + strand, within Dsui_2040 at 2159.615 kb on + strand, within Dsui_2040 at 2159.719 kb on + strand, within Dsui_2040 at 2159.854 kb on - strand, within Dsui_2040 at 2159.872 kb on + strand at 2159.876 kb on + strand at 2159.880 kb on - strand at 2159.945 kb on + strand at 2159.951 kb on - strand at 2159.978 kb on - strand at 2160.064 kb on + strand, within Dsui_2041 at 2160.110 kb on - strand, within Dsui_2041 at 2160.117 kb on - strand, within Dsui_2041 at 2160.119 kb on + strand at 2160.220 kb on - strand, within Dsui_2041 at 2160.246 kb on - strand, within Dsui_2041 at 2160.261 kb on + strand at 2160.269 kb on - strand, within Dsui_2041 at 2160.277 kb on - strand at 2160.324 kb on - strand, within Dsui_2041 at 2160.324 kb on - strand at 2160.460 kb on + strand at 2160.470 kb on + strand at 2160.506 kb on - strand, within Dsui_2041 at 2160.592 kb on - strand at 2160.625 kb on + strand, within Dsui_2041 at 2160.696 kb on - strand at 2160.765 kb on - strand at 2160.789 kb on - strand at 2160.789 kb on - strand at 2160.792 kb on + strand at 2160.792 kb on + strand at 2160.868 kb on + strand at 2160.876 kb on - strand at 2160.876 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Bacitracin 0.2 mg/ml remove 2,158,447 + Dsui_2039 0.25 +0.7 2,158,458 + Dsui_2039 0.26 -0.9 2,158,490 + Dsui_2039 0.28 -0.3 2,158,542 + Dsui_2039 0.32 +0.7 2,158,558 - Dsui_2039 0.33 +0.3 2,158,559 - Dsui_2039 0.33 +0.2 2,158,617 - Dsui_2039 0.38 +0.5 2,158,678 + Dsui_2039 0.43 -0.9 2,158,691 - Dsui_2039 0.44 +0.2 2,158,758 + +0.7 2,158,781 + +0.7 2,158,801 + Dsui_2039 0.52 -0.3 2,158,814 - Dsui_2039 0.53 +0.8 2,158,832 + Dsui_2039 0.55 +0.7 2,158,842 + Dsui_2039 0.55 -1.9 2,158,884 - Dsui_2039 0.59 -0.2 2,158,932 + Dsui_2039 0.62 +0.7 2,158,965 + -0.3 2,158,973 - Dsui_2039 0.66 +0.3 2,158,978 - Dsui_2039 0.66 +0.0 2,159,225 - Dsui_2039 0.85 -0.0 2,159,250 - Dsui_2039 0.87 +0.4 2,159,260 + Dsui_2039 0.88 -2.5 2,159,268 - Dsui_2039 0.89 +0.2 2,159,419 - +0.1 2,159,429 - +0.3 2,159,450 + +0.7 2,159,450 + -0.3 2,159,458 - +0.3 2,159,608 + Dsui_2040 0.36 -0.9 2,159,615 + Dsui_2040 0.38 -1.3 2,159,719 + Dsui_2040 0.60 +0.7 2,159,854 - Dsui_2040 0.90 +0.1 2,159,872 + +0.7 2,159,876 + +0.7 2,159,880 - -0.0 2,159,945 + -0.3 2,159,951 - +0.4 2,159,978 - +0.5 2,160,064 + Dsui_2041 0.13 -0.9 2,160,110 - Dsui_2041 0.19 +0.7 2,160,117 - Dsui_2041 0.20 -0.1 2,160,119 + -0.3 2,160,220 - Dsui_2041 0.33 +0.7 2,160,246 - Dsui_2041 0.36 -1.3 2,160,261 + +0.7 2,160,269 - Dsui_2041 0.39 +0.7 2,160,277 - +0.7 2,160,324 - Dsui_2041 0.46 -0.9 2,160,324 - -0.3 2,160,460 + -0.3 2,160,470 + +0.7 2,160,506 - Dsui_2041 0.69 +0.7 2,160,592 - -0.3 2,160,625 + Dsui_2041 0.84 -0.3 2,160,696 - -0.3 2,160,765 - -1.3 2,160,789 - -0.3 2,160,789 - -0.3 2,160,792 + +0.7 2,160,792 + -1.3 2,160,868 + -0.3 2,160,876 - +0.7 2,160,876 - +0.7
Or see this region's nucleotide sequence